FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s42.351000000d8066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s42.351000000d8066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883377
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT1876043.841685784243158No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT1397732.862220139874517RNA PCR Primer, Index 47 (95% over 22bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG1033982.117346254446462RNA PCR Primer, Index 47 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT827531.6945855296447518RNA PCR Primer, Index 47 (95% over 24bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT380480.7791329647495985RNA PCR Primer, Index 47 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC308180.6310796811304964No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA264670.541981501735377RNA PCR Primer, Index 47 (96% over 29bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG202910.415511642865173TruSeq Adapter, Index 9 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT180190.3689864616227664RNA PCR Primer, Index 47 (95% over 21bp)
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC89310.1828857366531398Illumina Paired End PCR Primer 2 (95% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87250.17866734433978781No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT84380.17279026378671972No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT59740.1223333770872083No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.11852453742563804No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG54840.11229933711855546No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA53700.10996488700339949No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT52670.10785569084672349No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.10435401567398953No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA50150.10269532743427344No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC49430.10122093788785914No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT49030.10040183258429565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT263550.030.7294334
CTTCCGA266700.030.694721
TTCCGAT269650.030.2985062
TCCGATC272200.029.822713
CGATCTT129050.025.9124135
TTTAGGG15000.024.6999743
TTAGGGA14750.024.4745524
GATCTTT109650.023.5658686
GTATAAG12100.023.1599488
GATCTAG13850.022.9783176
ATCTTTT115000.022.8401157
ACTTTAG19000.021.2676181
GTGTATA13050.020.3831216
AGGACTA45350.020.0054935
TCTTTTT126450.019.6826428
GTTAATA15450.019.384991
GTCCTAG39050.019.3566251
TCTTCCG186600.019.269815
CTTTAGG27950.019.033972
CTAGGAC47000.018.999983
Produced by FastQC (version 0.11.2)