FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s41.351000000d804d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s41.351000000d804d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA1674.937906564163217Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA1103.252513305736251Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT722.1289178001182734No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG671.9810762862211708Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC591.7445298639858071Illumina PCR Primer Index 10 (96% over 25bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA351.034890597279716Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA341.0053222945002958Illumina PCR Primer Index 10 (96% over 28bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG320.9461856889414548Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.9166173861620343No Hit
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA290.8574807806031934Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT270.7983441750443524No Hit
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT260.768775872264932Illumina PCR Primer Index 10 (96% over 27bp)
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG260.768775872264932Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG260.768775872264932TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT250.7392075694855115TruSeq Adapter, Index 10 (95% over 21bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA230.6800709639266705Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG220.6505026611472502Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG210.6209343583678296Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG210.6209343583678296TruSeq Adapter, Index 10 (96% over 28bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA200.5913660555884093Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT180.5322294500295683No Hit
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT160.4730928444707274TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG160.4730928444707274TruSeq Adapter, Index 10 (96% over 28bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC150.4435245416913069No Hit
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT140.4139562389118865Illumina PCR Primer Index 10 (96% over 26bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT130.384387936132466Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT130.384387936132466No Hit
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT130.384387936132466RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT130.384387936132466Illumina PCR Primer Index 10 (95% over 23bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA110.3252513305736251Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT110.3252513305736251No Hit
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA110.3252513305736251Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.29568302779420463RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.29568302779420463Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT100.29568302779420463Illumina PCR Primer Index 10 (96% over 26bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT100.29568302779420463RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT100.29568302779420463TruSeq Adapter, Index 10 (96% over 26bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA100.29568302779420463Illumina PCR Primer Index 11 (100% over 29bp)
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT100.29568302779420463TruSeq Adapter, Index 10 (96% over 26bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA90.26611472501478417TruSeq Adapter, Index 11 (96% over 30bp)
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG90.26611472501478417Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT90.26611472501478417RNA PCR Primer, Index 47 (95% over 22bp)
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA90.26611472501478417RNA PCR Primer, Index 10 (96% over 28bp)
TCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG90.26611472501478417TruSeq Adapter, Index 10 (96% over 28bp)
TCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA90.26611472501478417Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT80.2365464222353637Illumina PCR Primer Index 10 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT80.2365464222353637No Hit
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT80.2365464222353637Illumina PCR Primer Index 10 (95% over 21bp)
CACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG70.20697811945594324Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.20697811945594324RNA PCR Primer, Index 29 (96% over 28bp)
ATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTT70.20697811945594324TruSeq Adapter, Index 10 (95% over 21bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.20697811945594324Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTG60.17740981667652278Illumina PCR Primer Index 10 (95% over 24bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.17740981667652278Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT60.17740981667652278Illumina PCR Primer Index 10 (95% over 23bp)
TTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC60.17740981667652278No Hit
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT60.17740981667652278Illumina PCR Primer Index 10 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA60.17740981667652278No Hit
CATCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT60.17740981667652278Illumina PCR Primer Index 10 (96% over 27bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.17740981667652278Illumina PCR Primer Index 10 (96% over 28bp)
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.17740981667652278RNA PCR Primer, Index 10 (96% over 28bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA60.17740981667652278Illumina PCR Primer Index 10 (96% over 28bp)
TACACATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.14784151389710232Illumina PCR Primer Index 10 (95% over 23bp)
TCTCTGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG50.14784151389710232Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.14784151389710232TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.14784151389710232RNA PCR Primer, Index 29 (96% over 28bp)
CATATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT50.14784151389710232Illumina PCR Primer Index 10 (96% over 26bp)
CTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.14784151389710232Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.14784151389710232Illumina PCR Primer Index 10 (96% over 27bp)
TCTCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTG50.14784151389710232TruSeq Adapter, Index 12 (96% over 26bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG50.14784151389710232Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA50.14784151389710232Illumina PCR Primer Index 11 (96% over 29bp)
TTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC50.14784151389710232No Hit
TCTCCGAGCCCCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.14784151389710232RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.11827321111768185Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.11827321111768185TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG40.11827321111768185No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11827321111768185No Hit
TCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAA40.11827321111768185RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACGAGAAACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.11827321111768185TruSeq Adapter, Index 10 (96% over 28bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG40.11827321111768185TruSeq Adapter, Index 9 (95% over 24bp)
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.11827321111768185RNA PCR Primer, Index 29 (96% over 28bp)
AGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG40.11827321111768185Illumina PCR Primer Index 10 (95% over 24bp)
CACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG40.11827321111768185Illumina PCR Primer Index 10 (95% over 24bp)
ATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTT40.11827321111768185TruSeq Adapter, Index 10 (96% over 27bp)
CATCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT40.11827321111768185Illumina PCR Primer Index 10 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT40.11827321111768185Illumina PCR Primer Index 10 (95% over 21bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.11827321111768185TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTC40.11827321111768185Illumina PCR Primer Index 10 (95% over 22bp)
TTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.11827321111768185Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCTACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.11827321111768185TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTC40.11827321111768185No Hit
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.11827321111768185TruSeq Adapter, Index 11 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGCATCTCCGAGCCCACGAGACACTC40.11827321111768185No Hit
ACACATCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT40.11827321111768185RNA PCR Primer, Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTG40.11827321111768185TruSeq Adapter, Index 12 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCCT156.0519954E-495.02
GCACCCC156.0519954E-495.01
TCTCGAG200.00189765471.251
TCGAGCC200.00189765471.253
CTCGAGC200.00189765471.252
AGAGACA205.011555E-447.50000418-19
CGCTGCA150.0098862447.520-21
AGCTCCG150.0098862447.524-25
TATAAGA150.0098862447.514-15
TAAGAGA150.0098862447.516-17
TACGCTG150.0098862447.518-19
AGACTAC150.0098862447.514-15
CTGCATC150.0098862447.522-23
GCATCTC150.0098862447.524-25
TGTATAA150.0098862447.512-13
TGTGTAT150.0098862447.510-11
ACTACGC150.0098862447.516-17
ACAGCTC150.0098862447.522-23
TCTCCGA1200.043.5416681
CTCCGAG1455.456968E-1236.034482
Produced by FastQC (version 0.11.2)