FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s40.351000000d8023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s40.351000000d8023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT32.7777777777777777Illumina PCR Primer Index 11 (95% over 22bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG21.8518518518518516Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT21.8518518518518516No Hit
TAGCTACCCAGCTCTGCCATGGGCATGACAACTGGTGCACCAGCGGTGCG10.9259259259259258No Hit
CCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGA10.9259259259259258No Hit
ACGTAGAACCTATTCTGACCAACAAGTGTCACAGGAATAGCCTTCGCCCA10.9259259259259258No Hit
CCCCGGAAGGGGAGTGAAATAGAACTTGAAACCGTGTGCTTACAAGTAGT10.9259259259259258No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.9259259259259258No Hit
GTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTATTGTTAGTTGCCAGCA10.9259259259259258No Hit
GTGCTGGAAGGTTAAGAGGAGTGGTTAGCTTCTGCGAAGCCACGAATCGG10.9259259259259258No Hit
CTCTATCTCTAGAGCGGTCAAAGGATGTCAAGATTTGGTAAGGTTCTTCG10.9259259259259258No Hit
TATCACATGTTCTCACTTATAAGTGGGAGCTAAATGATGAGAACACATGG10.9259259259259258No Hit
GTCCAGCAGCACGCGTCGCGGCCCGGGCCTCATGGTTCGAGATGCGGCCT10.9259259259259258No Hit
ACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACC10.9259259259259258No Hit
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT10.9259259259259258No Hit
GTACAAGACTCGGGAACATTTTCACCGTAGCATGCTGATCTACGATTACT10.9259259259259258No Hit
CTCCTATCCGTGTTCTTCCCCAACAACAGAGCTTTACGAACCGAAGTCCT10.9259259259259258No Hit
ATTCACTGCGGCTGACTTGCGTCAACGCCCCTTCTCCCGAAGTTACGGGG10.9259259259259258No Hit
ACGCATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT10.9259259259259258Illumina PCR Primer Index 11 (95% over 24bp)
CTCTTAGGAAATCAGTTTAAATTTGTTGGAGGATTTAAATTCTCATGATT10.9259259259259258No Hit
GTACCATACTCAGGCACACAAAAATACTGATAGCAGTCGGCGTGTGAATC10.9259259259259258No Hit
AAATGAACCGGGGATATACCCTCACGGAGGTCTTGATTGCGGTGGCCATC10.9259259259259258No Hit
GTGTAACGGAGGCGCCCAAAGGTTCCCTCAGAATGGTTGGAAATCATTCG10.9259259259259258No Hit
CCGTATATCCGCGCAAAATAGCAATCGAAAAACGTTTTTCACAATTGCCG10.9259259259259258No Hit
ATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGT10.9259259259259258No Hit
TCACTCGCCGGTCCATTCTACAAAAGGTACGCCATCACCCATTAACGGGC10.9259259259259258No Hit
GTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGT10.9259259259259258No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCTGTCTCTT10.9259259259259258No Hit
ACAAGTCGAGCAGGGACGAAAGTCGGGCTTAGTGATCCGGTGGTTCCGCA10.9259259259259258No Hit
GTATTCACTCGAGGCGCTACCTAAATAGCTTTTGCGGAGAACCAGCTATT10.9259259259259258No Hit
GTAACCGACCGAGCGGTCGGCCCATGAGGATCCTGAGTACGACGGCACAC10.9259259259259258No Hit
GAGATAGAGTCTTCCCCTTCGGGGGACAAAGTGACAGGTGGTGCATGGTT10.9259259259259258No Hit
GGACGTTACTAACGACGATATGCTTTGGGGAGCTGTAAGTAAGCTGTGAT10.9259259259259258No Hit
GCAGTTTGACTGGGGCGGTCGCCTCCCAAAGTGTAACGGAGGCGCCCAAA10.9259259259259258No Hit
CTTCCGATCTGGACGGGGTGCTCTTCCGAAACTGTCTCTTATACACATCT10.9259259259259258No Hit
ATATTGAGAACTTTGCTGCTAATACTGGAAACAAATAAATAAGAATGTCT10.9259259259259258No Hit
GTAGGACTCAATGTCGGGACTGACCGTTTAAGGTGAACACCCTGGGAAGG10.9259259259259258No Hit
GATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAGTTGCAGACTA10.9259259259259258No Hit
TAATCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTA10.9259259259259258No Hit
CCCCAAGAGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTC10.9259259259259258No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG10.9259259259259258No Hit
GTGCTATAATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAGATCGTTGC10.9259259259259258No Hit
GCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGGGA10.9259259259259258No Hit
GGCCCTTCCATGCGGAACCACCGGATCACTAAGTCCGTCTTTCGACCCTG10.9259259259259258No Hit
GGCAAAGGGCACAGACCCCACACTCCTCAGCAGCCCATGCTTCTCCAAGG10.9259259259259258No Hit
CTACAATGGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCC10.9259259259259258No Hit
AATTTGAACGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCG10.9259259259259258No Hit
GGGACTACAGGCGCCTGCCACCACGCCCATCTAATTTTTGTATTTTTAGT10.9259259259259258No Hit
GTTTGTGTCCTCTCTTATTTCCTTGAGCAGTGGTTTGTAGTTCTCCTTGA10.9259259259259258No Hit
GGATTACACGTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCAT10.9259259259259258No Hit
GCACACGGTTTCAAGTTCTATTTCACTCCCCTTCCGGGGTGCTTTTCACC10.9259259259259258No Hit
CCCTAACCTTCGTTCTTGATTAATGAAAACATCGGGCAAATGCTTTCGCA10.9259259259259258No Hit
GGTTGACGCCGGAATTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGG10.9259259259259258No Hit
GATCTACACCGGGCAGGTGTCAGCCCCTATACGTCTTCTTTCGAATTAGC10.9259259259259258No Hit
GAGCTGGGTAGCTATGTGCGGGAAGGATAAGCGCTGAAAGCATCTAAGCG10.9259259259259258No Hit
CCACGGTTGAGCCGTGGGCTTTCACATCAGACTTAAAAAACCGCCTACGC10.9259259259259258No Hit
TACCTATCCTGTACAAGCTGCGCCGAATTTCAATATCAGGCTACAGTAAA10.9259259259259258No Hit
GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAGTGATGTA10.9259259259259258No Hit
GTAGTGGCTCACGCCTGTAATCCTAACACTTTGGGAGGCTGAGGCAGGCA10.9259259259259258No Hit
GAATAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTTTGGAATCGC10.9259259259259258No Hit
GCTGATGTGCGAGCTCAACCCGGAGACGGGCGTCAACAAGGTGGCCGAAA10.9259259259259258No Hit
ACGAGAGCGGTCAGTAGCAATCCAAACATTGTTACTCGTCAGAAAATCGA10.9259259259259258No Hit
CTATTTAGGTAGCGCCTCGAGTGAATACCTCACGGGGTAGAGCACTGCTG10.9259259259259258No Hit
GTGCAGCGACGATCAGCAAAAATTACACGTGATATCTTTTTCTTTTGCCT10.9259259259259258No Hit
ATCTACCATGGGTGTTACAAACTCTCGTGGTGTGACGGGCGGTGTGTACA10.9259259259259258No Hit
GAGGATAACTCCTATACTATAGGAAGTAAGGAGAATCTTGAGCTACTACT10.9259259259259258No Hit
CCGTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCG10.9259259259259258No Hit
TTACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGA10.9259259259259258No Hit
TGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTC10.9259259259259258No Hit
CACCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGCAAGTTG10.9259259259259258No Hit
CCCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCCCGTCGATAT10.9259259259259258No Hit
ATTTCCAACCATTCTGAGGGAACCTTTGAGCGCCTCCGTTACCTTTTAGG10.9259259259259258No Hit
AAATTATAGTACCTGTGAAGATGCAGGTTACCCGCGACAGGACGGAAAGA10.9259259259259258No Hit
CTTCCGATCTGTGCGGGGGGCTCAGGACGAACGCTGGCGGCGTGCTTAAC10.9259259259259258No Hit
ACAATTTAGACATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCA10.9259259259259258No Hit
CTTTGATTTCTCGTAAGGTGCCGATCGAGTCAAAAAGATAACATCGACCG10.9259259259259258No Hit
GAAAGCATCTAAGCGTGAAACTTGCCTTAAGATAAGATTTCCCTGAGGAC10.9259259259259258No Hit
TGTCGGGACCGGCCGTTTAAGGTGAACACCCTGGGAAGGGTGGCCAGAGC10.9259259259259258No Hit
CTGTTTGGACGTGCACTCCAACAGCACGCTTCGCCTATCCTACTGCGTCC10.9259259259259258No Hit
CCGAAAATGTACCGGGGCTAAACAAGGTACCGAAGCCACGGATAGAATTT10.9259259259259258No Hit
CCTCATTACCATACTAGGAAGTAAGCACTATTATTATCCCCATTTTGCAG10.9259259259259258No Hit
CTACAAAAGGTACGCCATCACCCATTAACGGGCTCTGACTACTTGTAAGC10.9259259259259258No Hit
GGTGGGATACTACCCTAACGGTATGACCCCTCTAACTCGCCACCCTAACC10.9259259259259258No Hit
GACAACTGGTGCACCAGCGGTGCGTCCATCCCGGTCCTCTCGTACTAAGG10.9259259259259258No Hit
GGGTTAGTCGGGTCCTAAGCTGAGGCCGACAGGCGTAGGCGATGGATAAC10.9259259259259258No Hit
CTGTGAAGATGCAGGTTACCCGCGACAGGACGGAAAGACCCCGTGGAGCT10.9259259259259258No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTG10.9259259259259258Illumina PCR Primer Index 11 (96% over 25bp)
GCCATGGGGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACTTACCTA10.9259259259259258No Hit
CTAGGGAGTATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGGCAT10.9259259259259258No Hit
ATGTGGAGCCGTAGCGAAAGCGAGTCTGAATAGGGCGCTGAGTATTTGCT10.9259259259259258No Hit
TACAACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT10.9259259259259258Illumina PCR Primer Index 11 (95% over 22bp)
TCGGGGAGCTGTAAGTAAGCTTTGATCCGGAGATGTCCGAATGGGGCAAC10.9259259259259258No Hit
GGCCCAGCTAGATGTGACAGTCAGTAGTACGCGAACTTCCGGCAGGCAAC10.9259259259259258No Hit
ATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAG10.9259259259259258No Hit
AGGCATGGTGACTCTCTTCTGTAATCCCAGCACTTTGGGAGGCTGAGGTG10.9259259259259258No Hit
GCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTAT10.9259259259259258No Hit
GGCTGGGCTACAGACGTGCTACAATGGTGGTGACAGTGGGCAGCGAGACA10.9259259259259258No Hit
AGTTTAAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATCTGTCTC10.9259259259259258No Hit
CATCAGATCAATCAGATTGTTCATGTTCTCTTTGCTCCCTCAGGTTGATT10.9259259259259258No Hit
ATTTCACCGAGTCTCTCGTTTAGACAGTGCCCAAATCGTTACGCCTTTCG10.9259259259259258No Hit
CTAAATCACTACATAAAAGATAACTTAAGCTGTTTTTTGGTTTTTTTTTT10.9259259259259258No Hit
GGACGGAAAGACCCCATGGAGCTTTACTGTAGCTTGATATTGAGTGTTTG10.9259259259259258No Hit
TATCAAGGCTGCCCAGGACATGAACTTGGAACCCAGCCAAGTAACAAGCC10.9259259259259258No Hit
GACCTCAGGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAG10.9259259259259258No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers