HG5M3BCX2_l01n01_r3zt2s4.351000000d7b88.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s4.351000000d7b88.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2022
Sequences flagged as poor quality	0
Sequence length	101
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA	105	5.192878338278932	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	56	2.7695351137487636	Illumina PCR Primer Index 7 (96% over 27bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	35	1.7309594460929771	Illumina PCR Primer Index 7 (96% over 27bp)
TCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	23	1.1374876360039565	RNA PCR Primer, Index 41 (96% over 31bp)
AGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA	19	0.9396636993076163	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	16	0.791295746785361	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	14	0.6923837784371909	RNA PCR Primer, Index 41 (95% over 21bp)
TCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	14	0.6923837784371909	Illumina PCR Primer Index 7 (96% over 27bp)
TCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	13	0.6429277942631058	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	12	0.5934718100890208	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	11	0.5440158259149357	Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGT	10	0.4945598417408506	RNA PCR Primer, Index 41 (95% over 21bp)
CATCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	10	0.4945598417408506	Illumina PCR Primer Index 7 (96% over 26bp)
TCTCCGAGCCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	10	0.4945598417408506	Illumina PCR Primer Index 3 (96% over 26bp)
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTT	10	0.4945598417408506	RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	10	0.4945598417408506	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	9	0.44510385756676557	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTC	8	0.3956478733926805	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	8	0.3956478733926805	RNA PCR Primer, Index 41 (96% over 26bp)
TCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	8	0.3956478733926805	RNA PCR Primer, Index 41 (96% over 31bp)
CGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAA	8	0.3956478733926805	Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	7	0.34619188921859545	No Hit
ATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	7	0.34619188921859545	RNA PCR Primer, Index 41 (95% over 24bp)
CTTATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	7	0.34619188921859545	RNA PCR Primer, Index 41 (95% over 21bp)
TCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	7	0.34619188921859545	Illumina PCR Primer Index 3 (96% over 27bp)
CCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7	0.34619188921859545	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	7	0.34619188921859545	Illumina PCR Primer Index 7 (96% over 27bp)
ACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	6	0.2967359050445104	RNA PCR Primer, Index 41 (96% over 26bp)
CTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	6	0.2967359050445104	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	6	0.2967359050445104	RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	6	0.2967359050445104	RNA PCR Primer, Index 41 (96% over 26bp)
ATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	6	0.2967359050445104	RNA PCR Primer, Index 41 (95% over 24bp)
CTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.2472799208704253	Illumina PCR Primer Index 7 (96% over 27bp)
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT	5	0.2472799208704253	No Hit
CTTATACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGT	5	0.2472799208704253	RNA PCR Primer, Index 41 (95% over 21bp)
CTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.2472799208704253	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTC	5	0.2472799208704253	RNA PCR Primer, Index 41 (95% over 22bp)
TCTCCGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.2472799208704253	Illumina PCR Primer Index 3 (96% over 27bp)
CATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	5	0.2472799208704253	RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.2472799208704253	Illumina PCR Primer Index 3 (96% over 27bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTCGACGT	4	0.19782393669634024	No Hit
CTCCGAGCCCACCGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	4	0.19782393669634024	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.19782393669634024	RNA PCR Primer, Index 41 (96% over 29bp)
CCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA	4	0.19782393669634024	Illumina PCR Primer Index 7 (96% over 27bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	4	0.19782393669634024	No Hit
ATACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTTC	4	0.19782393669634024	Illumina PCR Primer Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTC	4	0.19782393669634024	RNA PCR Primer, Index 41 (95% over 22bp)
CTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGA	4	0.19782393669634024	RNA PCR Primer, Index 7 (96% over 26bp)
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT	4	0.19782393669634024	No Hit
CATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.19782393669634024	Illumina PCR Primer Index 7 (96% over 25bp)
TCTCCGAGCCCCGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.19782393669634024	Illumina PCR Primer Index 3 (96% over 27bp)
CTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.19782393669634024	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTG	4	0.19782393669634024	TruSeq Adapter, Index 7 (96% over 26bp)
CTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.19782393669634024	RNA PCR Primer, Index 41 (96% over 31bp)
TGTGTAGCCCAAATCATAAGGGGCATGATGATTTGACGTCATCCCCACCT	3	0.1483679525222552	No Hit
CTCCGAGCCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.1483679525222552	RNA PCR Primer, Index 41 (96% over 31bp)
ACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTG	3	0.1483679525222552	RNA PCR Primer, Index 41 (96% over 27bp)
AAGTAACTGTGCGCGTCTTGACGGTACCTAATCAGAAAGCCACGGCTAAC	3	0.1483679525222552	No Hit
CTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.1483679525222552	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.1483679525222552	RNA PCR Primer, Index 41 (96% over 31bp)
TTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTC	3	0.1483679525222552	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	3	0.1483679525222552	Illumina PCR Primer Index 10 (95% over 21bp)
CTTGTAAGCATACGGTTTCAGGTGCTCTTTCACTCCCCTCCCGGGGTTCT	3	0.1483679525222552	No Hit
CATCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	3	0.1483679525222552	RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCTGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	3	0.1483679525222552	No Hit
TCTCCGAGCCCACGAAACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.1483679525222552	RNA PCR Primer, Index 41 (96% over 31bp)
GCCCAAATCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCC	3	0.1483679525222552	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.1483679525222552	No Hit
CATTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	3	0.1483679525222552	Illumina PCR Primer Index 7 (96% over 26bp)
TCTCCGAGCCCACGAGGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.1483679525222552	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCCCCGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	3	0.1483679525222552	Illumina PCR Primer Index 3 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGACTCGACGTCATCTCGTATGCCGTCT	3	0.1483679525222552	No Hit
CTTATACACATCTCCGAGCCCATGAGACTCGACGTCATCTCGTATGCCGT	3	0.1483679525222552	RNA PCR Primer, Index 41 (95% over 21bp)
GTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGTCCTCCC	3	0.1483679525222552	No Hit
TCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.1483679525222552	Illumina PCR Primer Index 3 (96% over 27bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	45	1.035943E-5	52.777775	3
TTATACA	45	1.035943E-5	52.777775	2
CACATCT	50	1.934408E-5	47.5	7
ACACATC	50	1.934408E-5	47.5	6
CTTATAC	50	1.934408E-5	47.5	1
CATCTCC	55	3.4007207E-5	43.18182	9
ACATCTC	55	3.4007207E-5	43.18182	8
TACACAT	60	5.6881247E-5	39.583336	5
ATACACA	60	5.6881247E-5	39.583336	4
CGAGACT	100	1.05592335E-8	23.75	12-13
CACGAGA	95	1.9158688E-7	22.500002	10-11
ATCTCCG	55	0.0024111967	21.59091	10-11
AGACTCG	110	2.9138391E-8	21.59091	14-15
TCATCTC	105	5.0402014E-7	20.357143	24-25
TCTCCGA	70	0.009748365	20.357141	1
TCGACGT	125	1.1308475E-7	19.0	18-19
TATGCCG	125	1.1308475E-7	19.0	32-33
CCGTCTT	125	1.1308475E-7	19.0	36-37
TCTGCTT	125	1.1308475E-7	19.0	42-43
GACGTCA	125	1.1308475E-7	19.0	20-21

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content