FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s37.351000000d7fd4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s37.351000000d7fd4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG712.888527257933279TruSeq Adapter, Index 7 (96% over 28bp)
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA582.3596419853539463RNA PCR Primer, Index 7 (96% over 28bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA562.2782750203417415Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAA431.7493897477624083TruSeq Adapter, Index 7 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC411.6680227827502034RNA PCR Primer, Index 29 (96% over 27bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA281.1391375101708707Illumina PCR Primer Index 10 (96% over 29bp)
GAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAA271.098454027664768RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA240.9764035801464606RNA PCR Primer, Index 42 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG220.8950366151342555RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACTAGCGCTCACCTCGTATGCCGTCTTCTGCTTG130.5288852725793327Illumina PCR Primer Index 7 (96% over 26bp)
TCTCCGAGCCCACGAGACTAGCGCTCAACTCGTATGCCGTCTTCTGCTTG100.40683482506102525Illumina PCR Primer Index 7 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTAGCGCT100.40683482506102525No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA100.40683482506102525RNA PCR Primer, Index 29 (96% over 30bp)
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA90.3661513425549227RNA PCR Primer, Index 29 (96% over 30bp)
TCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG80.32546786004882017TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT80.32546786004882017No Hit
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG80.32546786004882017TruSeq Adapter, Index 10 (96% over 29bp)
ACACATCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT70.2847843775427177RNA PCR Primer, Index 29 (95% over 22bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA70.2847843775427177Illumina PCR Primer Index 10 (96% over 28bp)
CCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAA70.2847843775427177RNA PCR Primer, Index 7 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2847843775427177No Hit
ACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT70.2847843775427177RNA PCR Primer, Index 29 (95% over 22bp)
TCTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTG70.2847843775427177Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGT70.2847843775427177No Hit
ATACACATCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT60.24410089503661514TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT60.24410089503661514No Hit
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.24410089503661514TruSeq Adapter, Index 10 (96% over 29bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA60.24410089503661514RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT60.24410089503661514Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT50.20341741253051263Illumina PCR Primer Index 7 (95% over 23bp)
ACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT50.20341741253051263Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACTAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG50.20341741253051263TruSeq Adapter, Index 7 (96% over 28bp)
CACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTG50.20341741253051263RNA PCR Primer, Index 29 (96% over 26bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT50.20341741253051263TruSeq Adapter, Index 10 (95% over 22bp)
AGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA50.20341741253051263Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT50.20341741253051263RNA PCR Primer, Index 29 (96% over 29bp)
TCTCCGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG50.20341741253051263Illumina Paired End PCR Primer 2 (96% over 29bp)
TCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA50.20341741253051263Illumina Paired End PCR Primer 2 (96% over 29bp)
AGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.16273393002441008TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT40.16273393002441008No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.16273393002441008No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTG40.16273393002441008Illumina PCR Primer Index 12 (96% over 26bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA40.16273393002441008Illumina PCR Primer Index 10 (96% over 29bp)
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.16273393002441008RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT40.16273393002441008TruSeq Adapter, Index 7 (96% over 27bp)
TCTCCGAGCCCACTAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG40.16273393002441008TruSeq Adapter, Index 5 (96% over 28bp)
TCTCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA40.16273393002441008TruSeq Adapter, Index 7 (96% over 28bp)
ACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT40.16273393002441008RNA PCR Primer, Index 10 (95% over 24bp)
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC40.16273393002441008Illumina Paired End PCR Primer 2 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT40.16273393002441008No Hit
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.16273393002441008Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGT30.12205044751830757No Hit
CTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTGA30.12205044751830757Illumina PCR Primer Index 7 (96% over 26bp)
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT30.12205044751830757RNA PCR Primer, Index 29 (96% over 29bp)
TCCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA30.12205044751830757TruSeq Adapter, Index 7 (96% over 28bp)
CGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAA30.12205044751830757Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG30.12205044751830757TruSeq Adapter, Index 5 (96% over 28bp)
CCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30.12205044751830757Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG30.12205044751830757Illumina PCR Primer Index 7 (96% over 28bp)
TCTCCAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.12205044751830757RNA PCR Primer, Index 29 (96% over 30bp)
CATTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT30.12205044751830757RNA PCR Primer, Index 7 (96% over 27bp)
CTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.12205044751830757Illumina PCR Primer Index 10 (96% over 29bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.12205044751830757Illumina PCR Primer Index 10 (96% over 29bp)
CCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAA30.12205044751830757RNA PCR Primer, Index 5 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG30.12205044751830757No Hit
TTATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC30.12205044751830757No Hit
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT30.12205044751830757No Hit
ATACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTT30.12205044751830757TruSeq Adapter, Index 10 (95% over 22bp)
ACACATCTCCGAGCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG30.12205044751830757Illumina PCR Primer Index 7 (95% over 24bp)
CACATCTCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGC30.12205044751830757TruSeq Adapter, Index 7 (96% over 25bp)
TCTCCGAGCCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT30.12205044751830757Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGATACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG30.12205044751830757Illumina PCR Primer Index 7 (96% over 28bp)
ATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT30.12205044751830757TruSeq Adapter, Index 7 (95% over 21bp)
TCTCCGAGCCAACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG30.12205044751830757Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTT30.12205044751830757Illumina PCR Primer Index 7 (95% over 21bp)
TCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.12205044751830757TruSeq Adapter, Index 10 (96% over 29bp)
CATCTCAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT30.12205044751830757Illumina Paired End PCR Primer 2 (96% over 27bp)
GCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA30.12205044751830757Illumina PCR Primer Index 10 (96% over 29bp)
CATCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCT30.12205044751830757TruSeq Adapter, Index 7 (96% over 26bp)
CTTATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT30.12205044751830757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT201.4182844E-595.000014
ATCTTTT155.851054E-495.07
TTCCGAT155.851054E-495.02
GATCTTT155.851054E-495.06
TCCGATC155.851054E-495.03
TCTTTTT155.851054E-495.08
CTTCCGA155.851054E-495.01
CGATCTT155.851054E-495.05
TCCGAGC502.080355E-547.51
CTTTTTT300.00914712647.59
CGAGCCC553.6564044E-543.1818163
CCGAGCC553.6564044E-543.1818162
GAGCCCA1250.00451003619.04
AGCCCAC1300.005661607318.2692325
GCCCACG1300.005661607318.2692326
CCACGAG1350.007044364717.5925948
CCCACGA1400.0086929516.9642877
Produced by FastQC (version 0.11.2)