HG5M3BCX2_l01n01_r3zt2s36.351000000d7fc7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s36.351000000d7fc7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1065
Sequences flagged as poor quality	0
Sequence length	101
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA	52	4.882629107981221	RNA PCR Primer, Index 41 (96% over 31bp)
TCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	52	4.882629107981221	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	29	2.7230046948356805	Illumina PCR Primer Index 7 (96% over 27bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	15	1.4084507042253522	Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGT	9	0.8450704225352111	RNA PCR Primer, Index 41 (95% over 21bp)
TCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	9	0.8450704225352111	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	8	0.7511737089201878	RNA PCR Primer, Index 41 (96% over 31bp)
TCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.6572769953051643	RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	7	0.6572769953051643	RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.6572769953051643	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	7	0.6572769953051643	RNA PCR Primer, Index 41 (95% over 21bp)
ATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	6	0.5633802816901409	RNA PCR Primer, Index 41 (95% over 24bp)
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTT	6	0.5633802816901409	RNA PCR Primer, Index 41 (95% over 24bp)
CATCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	6	0.5633802816901409	Illumina PCR Primer Index 7 (96% over 26bp)
ACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	5	0.4694835680751174	RNA PCR Primer, Index 41 (96% over 26bp)
CTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	5	0.4694835680751174	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTTCTGCTTG	5	0.4694835680751174	TruSeq Adapter, Index 7 (96% over 26bp)
AGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA	5	0.4694835680751174	Illumina PCR Primer Index 7 (96% over 27bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTCGACGT	4	0.3755868544600939	No Hit
CTTATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTC	4	0.3755868544600939	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	4	0.3755868544600939	RNA PCR Primer, Index 41 (96% over 26bp)
TCCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.3755868544600939	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.3755868544600939	RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.3755868544600939	Illumina PCR Primer Index 7 (96% over 25bp)
CCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA	4	0.3755868544600939	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	4	0.3755868544600939	RNA PCR Primer, Index 41 (96% over 31bp)
TATCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	4	0.3755868544600939	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.3755868544600939	Illumina PCR Primer Index 7 (96% over 27bp)
ACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	3	0.28169014084507044	RNA PCR Primer, Index 41 (96% over 26bp)
ACTTTATGGGATTTGCTTGACCTCGCGGTTTCGCTGCCCTTTGTATTGTC	3	0.28169014084507044	No Hit
TCTCCGAGCCCACGGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.28169014084507044	RNA PCR Primer, Index 41 (96% over 31bp)
CGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAA	3	0.28169014084507044	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	3	0.28169014084507044	RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	3	0.28169014084507044	Illumina PCR Primer Index 7 (96% over 25bp)
CTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.28169014084507044	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	3	0.28169014084507044	Illumina PCR Primer Index 3 (96% over 26bp)
TTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.18779342723004694	Illumina PCR Primer Index 7 (96% over 27bp)
GTCAAGCAAATCCCATAAAGTTGTTCTCAGTTCGGATTGTAGTCTGCAAC	2	0.18779342723004694	No Hit
GCCGTGGGCTTTCACATCAGACTTAAAAAACCGCCTACGCGCGCTTTACG	2	0.18779342723004694	No Hit
ATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACATATCTCGTAT	2	0.18779342723004694	No Hit
CTTATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTC	2	0.18779342723004694	RNA PCR Primer, Index 41 (95% over 22bp)
CTCCGATCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATATCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.18779342723004694	No Hit
GTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGC	2	0.18779342723004694	No Hit
CATTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.18779342723004694	Illumina PCR Primer Index 7 (96% over 26bp)
CTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGA	2	0.18779342723004694	RNA PCR Primer, Index 7 (96% over 26bp)
CACATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 27bp)
CTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTGA	2	0.18779342723004694	RNA PCR Primer, Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTC	2	0.18779342723004694	Illumina PCR Primer Index 3 (95% over 21bp)
GGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACATATCTCGTATGCCGT	2	0.18779342723004694	No Hit
ACACATCTCCGAGCCGACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 26bp)
CCCTTAAGCTTAGTTGCCATCATTCAGTTGGGCACTCTAAGTTGACTGCC	2	0.18779342723004694	No Hit
TTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTC	2	0.18779342723004694	No Hit
ATACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.18779342723004694	RNA PCR Primer, Index 41 (95% over 24bp)
AATCCGGACAACGCTTGTCACCTACGTATTACCGCGGCTGCTGGCACGTA	2	0.18779342723004694	No Hit
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT	2	0.18779342723004694	No Hit
CATCTGCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 29bp)
GTAAAACTCTGTTATTAGGGAAGAACAAATGCGTAAGTAACTGTGCGCGT	2	0.18779342723004694	No Hit
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT	2	0.18779342723004694	No Hit
TCTCCGGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 30bp)
TCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 29bp)
CACATTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC	2	0.18779342723004694	Illumina PCR Primer Index 7 (95% over 24bp)
CATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACAAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.18779342723004694	Illumina PCR Primer Index 7 (96% over 27bp)
CCCTGGGAAGGGTGGCCAGAGCAGGTGATAGCCCTGTAACCGACCGAGCG	2	0.18779342723004694	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	2	0.18779342723004694	No Hit
TCTCCAAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.18779342723004694	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.18779342723004694	Illumina PCR Primer Index 3 (96% over 27bp)
GTGTGTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGAT	2	0.18779342723004694	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAAA	370	3.8175458E-5	7.702703	52-53

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content