FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s35.351000000d7fae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s35.351000000d7fae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA993.3188065705665437Illumina PCR Primer Index 11 (100% over 29bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA581.9443513241702983TruSeq Adapter, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA391.3074086490110626RNA PCR Primer, Index 11 (96% over 30bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA381.2738853503184715Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG381.2738853503184715TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG321.0727455581629233RNA PCR Primer, Index 29 (96% over 30bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA280.9386523633925578Illumina PCR Primer Index 10 (96% over 29bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA240.8045591686221925RNA PCR Primer, Index 40 (96% over 29bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA230.771035869929601TruSeq Adapter, Index 11 (96% over 30bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA190.6369426751592357RNA PCR Primer, Index 11 (96% over 30bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA190.6369426751592357RNA PCR Primer, Index 29 (96% over 30bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC180.6034193764666443RNA PCR Primer, Index 29 (96% over 27bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC170.5698960777740529RNA PCR Primer, Index 11 (96% over 27bp)
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT160.5363727790814616No Hit
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG160.5363727790814616TruSeq Adapter, Index 11 (96% over 30bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA160.5363727790814616RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT150.5028494803888702TruSeq Adapter, Index 11 (95% over 23bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA150.5028494803888702No Hit
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG150.5028494803888702Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG120.4022795843110962TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT110.3687562856185049Illumina PCR Primer Index 11 (96% over 29bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG110.3687562856185049Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG110.3687562856185049TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG90.30170968823332217TruSeq Adapter, Index 10 (96% over 29bp)
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA90.30170968823332217TruSeq Adapter, Index 11 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.30170968823332217No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC90.30170968823332217RNA PCR Primer, Index 41 (96% over 26bp)
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA90.30170968823332217RNA PCR Primer, Index 29 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT90.30170968823332217No Hit
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG90.30170968823332217TruSeq Adapter, Index 11 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT90.30170968823332217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.2681863895407308No Hit
ACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCT80.2681863895407308Illumina PCR Primer Index 11 (96% over 25bp)
ACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT70.23466309084813944Illumina PCR Primer Index 10 (95% over 24bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT70.23466309084813944TruSeq Adapter, Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT70.23466309084813944No Hit
GGCTCGGAGATGTGTATAAGAGCTCCGAGCCCACGAGACGGAGCTACATC70.23466309084813944No Hit
CTTATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTC60.2011397921555481Illumina PCR Primer Index 11 (95% over 21bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA60.2011397921555481RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT60.2011397921555481TruSeq Adapter, Index 11 (96% over 29bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.2011397921555481Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT50.16761649346295676No Hit
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.16761649346295676Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGATGGAGCTACATCTCGTATGCCGTCTTCTGCTTG50.16761649346295676Illumina PCR Primer Index 11 (96% over 30bp)
ACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT50.16761649346295676Illumina PCR Primer Index 11 (100% over 24bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC50.16761649346295676RNA PCR Primer, Index 6 (96% over 25bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA50.16761649346295676Illumina PCR Primer Index 10 (96% over 29bp)
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA50.16761649346295676RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT50.16761649346295676Illumina PCR Primer Index 10 (95% over 22bp)
CTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA50.16761649346295676Illumina PCR Primer Index 11 (100% over 29bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT50.16761649346295676Illumina PCR Primer Index 11 (95% over 22bp)
TCTCCTAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG50.16761649346295676TruSeq Adapter, Index 6 (96% over 28bp)
CCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA50.16761649346295676Illumina PCR Primer Index 11 (100% over 29bp)
ACACATCTCCGAGCCCACAGACGGAGCTACATCTCGTATGCCGTCTTCTG40.1340931947703654Illumina PCR Primer Index 11 (96% over 26bp)
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT40.1340931947703654RNA PCR Primer, Index 29 (96% over 29bp)
CTTATACACATCTCCGAGCCCAGAGACGGAGCTACATCTCGTATGCCGTC40.1340931947703654RNA PCR Primer, Index 11 (95% over 21bp)
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.1340931947703654Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.1340931947703654Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGAGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.1340931947703654Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAACCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.1340931947703654Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.1340931947703654RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.1340931947703654Illumina PCR Primer Index 3 (96% over 28bp)
TCTCCGAGCCCACAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.1340931947703654Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.1340931947703654RNA PCR Primer, Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTC40.1340931947703654Illumina PCR Primer Index 11 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTGA40.1340931947703654Illumina PCR Primer Index 12 (96% over 26bp)
TCTCCGAGCCCACGATACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG40.1340931947703654RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.1340931947703654TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCGAGCCCACCGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT40.1340931947703654Illumina PCR Primer Index 11 (100% over 28bp)
GCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA40.1340931947703654Illumina PCR Primer Index 11 (100% over 29bp)
CTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.1340931947703654Illumina PCR Primer Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACAGACGGAGCTACATCTCGTATGCCGTCTTC40.1340931947703654Illumina PCR Primer Index 11 (95% over 24bp)
CTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.1340931947703654RNA PCR Primer, Index 11 (96% over 30bp)
CATCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.1340931947703654TruSeq Adapter, Index 11 (96% over 28bp)
CGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA40.1340931947703654TruSeq Adapter, Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGT30.10056989607777406No Hit
CTTATACACATCTCCGAGACCACGAGACGGAGCTACATCTCGTATGCCGT30.10056989607777406No Hit
ATACACATCTCCAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC30.10056989607777406TruSeq Adapter, Index 11 (95% over 24bp)
TCTCCGAGCCCACGAGACGGAGCTACAACTCGTATGCCGTCTTCTGCTTG30.10056989607777406Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT30.10056989607777406Illumina PCR Primer Index 11 (100% over 24bp)
CATCTCCGAGCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT30.10056989607777406Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGACCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.10056989607777406Illumina PCR Primer Index 11 (96% over 30bp)
CTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA30.10056989607777406Illumina PCR Primer Index 11 (96% over 30bp)
CATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT30.10056989607777406Illumina PCR Primer Index 11 (96% over 28bp)
GAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA30.10056989607777406TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT30.10056989607777406TruSeq Adapter, Index 11 (95% over 23bp)
CTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.10056989607777406Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTT30.10056989607777406Illumina PCR Primer Index 11 (95% over 23bp)
TCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.10056989607777406RNA PCR Primer, Index 29 (96% over 30bp)
CACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG30.10056989607777406Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT30.10056989607777406No Hit
CTTATACACATCTCCGAGCCCAAGAGACGGAGCTACATCTCGTATGCCGT30.10056989607777406No Hit
CTTATACACATCTCCGAGCCCACAGACGGAGCTACATCTCGTATGCCGTC30.10056989607777406Illumina PCR Primer Index 11 (95% over 21bp)
CATCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCT30.10056989607777406Illumina PCR Primer Index 11 (96% over 28bp)
TATCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG30.10056989607777406RNA PCR Primer, Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG30.10056989607777406TruSeq Adapter, Index 3 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGTCTTCTGC30.10056989607777406Illumina PCR Primer Index 12 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTC30.10056989607777406Illumina PCR Primer Index 11 (95% over 24bp)
TCTCCTAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG30.10056989607777406RNA PCR Primer, Index 41 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10056989607777406No Hit
TCTCCGAGCCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT30.10056989607777406RNA PCR Primer, Index 29 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT30.10056989607777406No Hit
TCTCCGAGCACACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG30.10056989607777406RNA PCR Primer, Index 40 (96% over 29bp)
CTTATACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGT30.10056989607777406No Hit
ATACACATCTCCGAGCCCACGAGAGGAGCTACATCTCGTATGCCGTCTTC30.10056989607777406TruSeq Adapter, Index 11 (95% over 24bp)
TCTCCGAGCCCACCGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT30.10056989607777406Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA458.1453356E-442.222222
CTTATAC458.1453356E-442.222221
TCTCCGA906.998699E-426.3888871
TCCGAGC1050.001722429822.6190473
CTCCGAG1050.001722429822.6190472
CGAGCCC1100.002258619521.590915
CCGAGCC1150.002925198320.6521744
AGCTACA900.002228341515.83333320-21
GGAGCTA900.002228341515.83333318-19
CTACATC900.002228341515.83333322-23
ATCTCGT2002.7145562E-410.68750126-27
TGCCGTC2002.7145562E-410.68750134-35
TATGCCG2053.4145045E-410.42682932-33
CCGTCTT2053.4145045E-410.42682936-37
TCTGCTT2053.4145045E-410.42682942-43
TGCTTGA2053.4145045E-410.42682944-45
GTCTTCT2053.4145045E-410.42682938-39
CGTATGC2104.2694504E-410.17857230-31
CTTGAAA2104.2694504E-410.17857246-47
CTCGTAT2104.2694504E-410.17857228-29
Produced by FastQC (version 0.11.2)