FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s34.351000000d7f84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s34.351000000d7f84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4157450
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT1085902.6119376059844375No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT836472.011978496434112Illumina PCR Primer Index 11 (95% over 22bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG658441.5837592755174446Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT483941.1640308362096958Illumina PCR Primer Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT223750.538190477335867Illumina PCR Primer Index 11 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC183630.4416890161036212No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA162010.3896859853997041Illumina PCR Primer Index 11 (96% over 29bp)
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTG125890.3028058064438538Illumina PCR Primer Index 11 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCT109240.262757218968358Illumina PCR Primer Index 11 (95% over 21bp)
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG82420.19824652130512693No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA72450.17426547523121144No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGC56190.1351549627776642Illumina PCR Primer Index 11 (96% over 26bp)
ATTCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGGCC49010.11788476109153448No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.1161288770760923No Hit
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT47100.11329059880455569No Hit
TCGTGAGACAGTTCGGTCCCTATCCGTCGCGGGCGTTGGAGATTTGAAGA46870.11273737507366294No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGC45680.10987504359643531No Hit
GTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACA45650.10980288397936236No Hit
CTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA44900.10799889355253822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT155350.058.584212
CCGATCT160200.056.2768824
CGATCTA27450.056.2389685
TCCGATC162650.055.60443
CTTCCGA185850.049.1223531
CGATCTT49950.047.9282235
CGATCTC43750.045.1659935
CGATCTG75350.042.1104135
GATCTAG11400.040.0002486
GATCTTT37000.039.1556476
TCTTCCG73700.037.832665
GATCTGC27250.035.036916
CTCTTCC82400.034.184074
GATCTCT16050.033.146626
GCTCTTC85850.032.589023
TGCTCTT89000.031.915932
GTGCTCT91100.031.8384531
GATCTGA22100.031.8101526
ATCTGTG7300.031.2311947
GATCTTC14650.031.1264746
Produced by FastQC (version 0.11.2)