FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s33.351000000d7f6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s33.351000000d7f6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3949667
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT808532.0470839693574168No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT483821.2249640286130452Illumina PCR Primer Index 11 (95% over 22bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG443761.1235377564741533Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT298520.7558105531428345Illumina PCR Primer Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT161580.4090977796355997Illumina PCR Primer Index 11 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC145680.36884122129789676No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA120950.30622834785818653No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT107440.2720229325662138No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA107050.27103550755038336Illumina PCR Primer Index 11 (96% over 29bp)
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT95530.24186849169816085No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG92500.2341969588828628No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA86730.21958813236660205No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA82560.20903028027426113No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT82010.20763775781603866No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCT78500.19875093267356464Illumina PCR Primer Index 11 (95% over 21bp)
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC76480.1936365774633659No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTG76090.19264915244753544Illumina PCR Primer Index 11 (96% over 25bp)
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC74870.18956028444929662No Hit
GTCTATTGTCTATTGTCTATTGTCTATTGTCTATTGTCTATTGTCTATTG58730.14869607994800577No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA53450.13532786434907043No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATA46840.1185922762602518No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT43430.10995863701927276No Hit
ACAATAGACAATAGACAATAGACAATAGACAATAGACAATAGACAATAGA42250.10697104338163192No Hit
GAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATA41400.10481896321892453No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT40320.10208455548277869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT56200.041.160344
CTTCCGA59500.040.6744461
TTCCGAT61550.038.6630332
TCCGATC61250.038.3095553
CGATCTG33100.027.4089035
TCTTCCG35450.026.9318455
CGATCTT28800.025.893665
GTGCTCT41300.023.3704341
GCTCTTC41200.023.0578613
CGATCTA14300.022.9191915
GATCTTT30200.020.4466746
TGCTCTT47900.020.0309772
ATAGGAC3707.2759576E-1219.256433
GTCCTAT4550.018.8097021
TAGGACT7450.018.489624
CGATCTC21650.017.9904585
GATCTGG20700.017.439326
GATCTAG9050.017.3201486
TAGGACA6400.016.3278484
TAATACT22700.015.9028144
Produced by FastQC (version 0.11.2)