FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s30.351000000d7f27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s30.351000000d7f27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6610541
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT1018781.5411446657694128No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT656930.9937613275524652Illumina PCR Primer Index 10 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640840.9694214134667647No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT506090.7655803057571233No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447540.6770096426298543No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT318060.48114065096941383Illumina PCR Primer Index 10 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281470.4257896592729703No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG270030.4084839652306823Illumina PCR Primer Index 10 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC205280.31053434204553No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT107720.16295186732825648Illumina PCR Primer Index 10 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT104970.15879184472193728Illumina PCR Primer Index 10 (96% over 27bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT95520.14449649431113126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT375250.036.404486
CGATCTT444700.035.354735
ATCTTTT391900.034.2871367
CCGATCT1075150.029.0257194
TCCGATC1099750.028.5492133
TTCCGAT1106650.028.5257262
CTTCCGA1125500.028.207181
TCTTTTT487450.027.5271918
GATCTGG98700.025.8911766
CTTTTTT520900.025.7321579
CGATCTG307100.025.597935
TGCTCTT779050.022.175052
GTGCTCT787900.022.0265031
GCTCTTC806450.021.3980713
TCTTCCG810850.021.328825
CGATCTA161250.021.2089635
CTCTTCC838750.020.6703114
ATCTGAC41250.020.265737
GATCTAG62100.020.0399366
GATCTGA95400.019.9656146
Produced by FastQC (version 0.11.2)