FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s3.351000000d7b7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s3.351000000d7b7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7652
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA2763.6069001568217463Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG1311.711970726607423RNA PCR Primer, Index 29 (96% over 30bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA1111.4506011500261369TruSeq Adapter, Index 11 (96% over 30bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA1081.411395713538944Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG781.0193413486670153TruSeq Adapter, Index 11 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.967067433350758No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA700.9147935180345007RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA650.8494511238891793RNA PCR Primer, Index 11 (96% over 30bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA580.7579717720857292RNA PCR Primer, Index 40 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC550.7187663355985363RNA PCR Primer, Index 11 (96% over 27bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA520.6795608991113434TruSeq Adapter, Index 11 (96% over 30bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA480.6272869837950863RNA PCR Primer, Index 17 (96% over 28bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA430.5619445896497648Illumina PCR Primer Index 10 (96% over 29bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA360.4704652378463147RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG350.45739675901725035Illumina PCR Primer Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG340.4443282801881861TruSeq Adapter, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC330.43125980135912184RNA PCR Primer, Index 29 (96% over 27bp)
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG290.3789858860428646TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT270.352848928384736TruSeq Adapter, Index 11 (95% over 23bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC250.3267119707266074RNA PCR Primer, Index 6 (96% over 25bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC240.31364349189754315RNA PCR Primer, Index 41 (96% over 26bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG230.30057501306847884TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT230.30057501306847884TruSeq Adapter, Index 11 (95% over 23bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA230.30057501306847884RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT230.30057501306847884No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA220.2875065342394145No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.27443805541035027No Hit
TCTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG210.27443805541035027TruSeq Adapter, Index 11 (96% over 30bp)
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA200.26136957658128596TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG200.26136957658128596TruSeq Adapter, Index 11 (96% over 30bp)
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT190.24830109775222162No Hit
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT190.24830109775222162No Hit
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG180.23523261892315736Illumina PCR Primer Index 3 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTT180.23523261892315736Illumina PCR Primer Index 11 (95% over 23bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG180.23523261892315736TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT180.23523261892315736Illumina PCR Primer Index 10 (95% over 21bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG180.23523261892315736Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT170.22216414009409305No Hit
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT170.22216414009409305TruSeq Adapter, Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT160.20909566126502874No Hit
CTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA160.20909566126502874Illumina PCR Primer Index 11 (96% over 30bp)
ACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT160.20909566126502874Illumina PCR Primer Index 11 (100% over 24bp)
ACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT150.19602718243596443Illumina PCR Primer Index 11 (100% over 24bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT150.19602718243596443No Hit
CGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA150.19602718243596443TruSeq Adapter, Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTC140.18295870360690017Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT140.18295870360690017No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.18295870360690017No Hit
ACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT130.16989022477783586RNA PCR Primer, Index 10 (95% over 24bp)
TCTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT130.16989022477783586Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT130.16989022477783586TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA130.16989022477783586Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTG130.16989022477783586Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGT130.16989022477783586No Hit
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG120.15682174594877157Illumina PCR Primer Index 11 (96% over 30bp)
CATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT110.14375326711970726Illumina PCR Primer Index 11 (96% over 28bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA110.14375326711970726Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTC110.14375326711970726Illumina PCR Primer Index 11 (95% over 21bp)
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT110.14375326711970726TruSeq Adapter, Index 11 (96% over 29bp)
AGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAA100.13068478829064298RNA PCR Primer, Index 41 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT100.13068478829064298No Hit
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG100.13068478829064298Illumina PCR Primer Index 11 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT100.13068478829064298Illumina PCR Primer Index 10 (95% over 22bp)
TCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG90.11761630946157868RNA PCR Primer, Index 29 (96% over 30bp)
GAGCCCACGAGACGGAGCTACACCTCGTATGCCGTCTTCTGCTTGAAAAA90.11761630946157868Illumina PCR Primer Index 11 (96% over 29bp)
TATCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG90.11761630946157868RNA PCR Primer, Index 11 (96% over 30bp)
GCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA90.11761630946157868Illumina PCR Primer Index 11 (100% over 29bp)
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA90.11761630946157868RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA90.11761630946157868Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG90.11761630946157868RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT90.11761630946157868No Hit
AGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA90.11761630946157868TruSeq Adapter, Index 6 (96% over 28bp)
CTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA90.11761630946157868Illumina PCR Primer Index 11 (100% over 29bp)
CATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT90.11761630946157868TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCT90.11761630946157868Illumina PCR Primer Index 11 (96% over 25bp)
ATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTC80.10454783063251437Illumina PCR Primer Index 11 (95% over 24bp)
ATACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTT80.10454783063251437TruSeq Adapter, Index 10 (95% over 22bp)
ACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCT80.10454783063251437Illumina PCR Primer Index 11 (96% over 25bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG80.10454783063251437Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA80.10454783063251437Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGT80.10454783063251437No Hit
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA80.10454783063251437RNA PCR Primer, Index 29 (96% over 30bp)
CATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT80.10454783063251437TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA80.10454783063251437Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGATCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG80.10454783063251437Illumina PCR Primer Index 11 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1009.094947E-1247.5000041
TCTCAGA502.5705343E-547.5000048
TTATACA1051.4551915E-1145.2380942
CTCAGAG554.514604E-543.181829
ATCTCAG607.543825E-539.5833367
TATACAC1206.184564E-1139.5833363
CATCTCA651.2089411E-436.5384646
TCTCCGA1701.8189894E-1233.5294151
TACACAT1559.622454E-1030.645165
CTCCGAG1951.0913936E-1129.230772
ATACACA1651.879016E-928.7878784
TCCGAGC2151.8189894E-1228.720933
CGAGCCC2251.8189894E-1227.4444455
CATCTCC1056.0659197E-527.1428579
ACACATC1656.3671905E-825.9090926
CCGAGCC2204.7293724E-1125.9090924
CACATCT1751.12646376E-724.428574
CAGAGCC550.002751023721.5909110-11
CGAGACG1804.1533876E-621.111118
GAGACGG1804.1533876E-621.111119
Produced by FastQC (version 0.11.2)