FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s29.351000000d7eff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s29.351000000d7eff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA231.9118869492934332Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC201.6625103906899419RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAA161.3300083125519535RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA141.1637572734829593RNA PCR Primer, Index 42 (96% over 28bp)
TCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAA110.914380714879468TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG100.8312551953449709TruSeq Adapter, Index 7 (96% over 28bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA90.7481296758104738Illumina PCR Primer Index 10 (96% over 29bp)
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA90.7481296758104738RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.5818786367414797RNA PCR Primer, Index 29 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.5818786367414797No Hit
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT50.41562759767248547Illumina PCR Primer Index 10 (95% over 22bp)
CCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAA50.41562759767248547RNA PCR Primer, Index 29 (96% over 30bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA50.41562759767248547Illumina PCR Primer Index 10 (96% over 29bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA50.41562759767248547RNA PCR Primer, Index 29 (96% over 30bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA40.3325020781379884Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTAGCGCT40.3325020781379884No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTGAGC40.3325020781379884No Hit
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC40.3325020781379884Illumina Paired End PCR Primer 2 (96% over 26bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3325020781379884No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT40.3325020781379884No Hit
CTTATACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGT40.3325020781379884No Hit
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT30.24937655860349126TruSeq Adapter, Index 10 (95% over 22bp)
CATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT30.24937655860349126TruSeq Adapter, Index 10 (96% over 27bp)
ATACACATCTCAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT30.24937655860349126Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.24937655860349126TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTT30.24937655860349126Illumina PCR Primer Index 7 (95% over 21bp)
AGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.24937655860349126Illumina Paired End PCR Primer 2 (96% over 29bp)
CCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAA30.24937655860349126RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.24937655860349126TruSeq Adapter, Index 10 (96% over 29bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.24937655860349126No Hit
TCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG30.24937655860349126TruSeq Adapter, Index 7 (96% over 28bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.24937655860349126RNA PCR Primer, Index 29 (96% over 30bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.24937655860349126Illumina PCR Primer Index 10 (96% over 29bp)
TCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG30.24937655860349126TruSeq Adapter, Index 7 (96% over 28bp)
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.24937655860349126RNA PCR Primer, Index 29 (96% over 30bp)
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA30.24937655860349126TruSeq Adapter, Index 5 (96% over 28bp)
CATCTCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT30.24937655860349126TruSeq Adapter, Index 7 (96% over 27bp)
TCTACGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG20.1662510390689942TruSeq Adapter, Index 5 (96% over 28bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTGAG20.1662510390689942No Hit
GATCGGAAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGC20.1662510390689942RNA PCR Primer, Index 7 (96% over 25bp)
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT20.1662510390689942RNA PCR Primer, Index 29 (96% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGTCTTCTGCTTG20.1662510390689942Illumina PCR Primer Index 12 (96% over 26bp)
CCCCTGGTGAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGC20.1662510390689942No Hit
CACATCTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG20.1662510390689942TruSeq Adapter, Index 7 (95% over 24bp)
TCTCCGAGTCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG20.1662510390689942TruSeq Adapter, Index 7 (96% over 28bp)
CCCCCTGCTAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGC20.1662510390689942No Hit
TCTCCGAGCACACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG20.1662510390689942Illumina PCR Primer Index 7 (96% over 28bp)
CATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT20.1662510390689942Illumina PCR Primer Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT20.1662510390689942No Hit
CTTATACACATCTCCGAGCCCACGAGCACTGAGCGATCTCGTATGCCGTC20.1662510390689942No Hit
GATCGGAAGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGTCTTCTGC20.1662510390689942Illumina PCR Primer Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGGACTAGCGCTCATCTCGTATGCCGTC20.1662510390689942No Hit
ATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT20.1662510390689942TruSeq Adapter, Index 7 (95% over 21bp)
CCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20.1662510390689942Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCAACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG20.1662510390689942Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT20.1662510390689942No Hit
CATCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCT20.1662510390689942TruSeq Adapter, Index 10 (96% over 27bp)
CGTCTCCCAGGTAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGT20.1662510390689942No Hit
CATCTCCGAGCACACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCT20.1662510390689942Illumina PCR Primer Index 7 (96% over 26bp)
AAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT20.1662510390689942Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT20.1662510390689942RNA PCR Primer, Index 29 (96% over 25bp)
CTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942RNA PCR Primer, Index 10 (96% over 29bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG20.1662510390689942RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT20.1662510390689942Illumina PCR Primer Index 10 (95% over 24bp)
CTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACTAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG20.1662510390689942TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGCTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942Illumina PCR Primer Index 7 (96% over 28bp)
AATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC20.1662510390689942Illumina PCR Primer Index 10 (96% over 26bp)
CGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG20.1662510390689942RNA PCR Primer, Index 5 (96% over 28bp)
CTTATACACATCTCCGAGCCCACAGACTAGCGCTCATCTCGTATGCCGTC20.1662510390689942No Hit
CTCCGAGCCCACGGGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942Illumina PCR Primer Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCAGAGACTGCAGCTAATCTCGTATGCCGTC20.1662510390689942RNA PCR Primer, Index 29 (95% over 21bp)
CGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA20.1662510390689942RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACTGCAGCTAAGCTCGTATGCCGTCTTCTGCTTG20.1662510390689942Illumina PCR Primer Index 12 (96% over 26bp)
GAATAGGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTGCTAAACCG20.1662510390689942No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCG20.1662510390689942No Hit
CGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAA20.1662510390689942TruSeq Adapter, Index 7 (96% over 28bp)
CTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942Illumina PCR Primer Index 12 (96% over 26bp)
CTCAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942Illumina Paired End PCR Primer 2 (96% over 29bp)
CTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942RNA PCR Primer, Index 10 (96% over 29bp)
CTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942RNA PCR Primer, Index 29 (96% over 27bp)
CTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA20.1662510390689942Illumina PCR Primer Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAACTGCAGCTAATCTCGTATGCCGTC20.1662510390689942No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA300.00800507547.4999961
CTCCGAG300.00800507547.4999962
Produced by FastQC (version 0.11.2)