FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s26.351000000d7e78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s26.351000000d7e78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA181.5734265734265735Illumina PCR Primer Index 5 (96% over 28bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA161.3986013986013985RNA PCR Primer, Index 40 (96% over 29bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA121.048951048951049Illumina PCR Primer Index 11 (96% over 29bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA110.9615384615384616Illumina PCR Primer Index 11 (100% over 29bp)
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA90.7867132867132868Illumina PCR Primer Index 9 (100% over 27bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC80.6993006993006993RNA PCR Primer, Index 12 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.6993006993006993No Hit
TCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAA70.6118881118881119TruSeq Adapter, Index 9 (96% over 28bp)
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT60.5244755244755245No Hit
TCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG60.5244755244755245TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG50.4370629370629371Illumina PCR Primer Index 12 (96% over 29bp)
TCTCCGAGCCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT50.4370629370629371RNA PCR Primer, Index 47 (96% over 28bp)
AGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA50.4370629370629371TruSeq Adapter, Index 12 (96% over 29bp)
CCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAA50.4370629370629371RNA PCR Primer, Index 12 (96% over 29bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG50.4370629370629371RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG50.4370629370629371Illumina PCR Primer Index 9 (96% over 28bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4370629370629371No Hit
TCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG50.4370629370629371TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCACACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG40.34965034965034963Illumina PCR Primer Index 12 (96% over 29bp)
TCTCCGAGCACACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG40.34965034965034963Illumina PCR Primer Index 9 (96% over 28bp)
TCTCCGAGCCCACTAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG40.34965034965034963TruSeq Adapter, Index 12 (96% over 29bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC40.34965034965034963RNA PCR Primer, Index 47 (96% over 26bp)
TCTCAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG40.34965034965034963RNA PCR Primer, Index 40 (96% over 29bp)
CATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT40.34965034965034963TruSeq Adapter, Index 9 (96% over 26bp)
TCTCCTAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG40.34965034965034963RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG40.34965034965034963RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG40.34965034965034963TruSeq Adapter, Index 12 (96% over 29bp)
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA40.34965034965034963TruSeq Adapter, Index 12 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTACGCTG30.26223776223776224No Hit
TCTCCGAGCCCACTAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG30.26223776223776224Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT30.26223776223776224Illumina PCR Primer Index 12 (96% over 27bp)
CATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT30.26223776223776224TruSeq Adapter, Index 12 (96% over 27bp)
AGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.26223776223776224TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGT30.26223776223776224No Hit
TCTCCGAGCACACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG30.26223776223776224TruSeq Adapter, Index 11 (96% over 29bp)
TCTCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA30.26223776223776224TruSeq Adapter, Index 6 (96% over 28bp)
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG30.26223776223776224TruSeq Adapter, Index 11 (96% over 29bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.26223776223776224TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGATACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG30.26223776223776224Illumina PCR Primer Index 12 (96% over 29bp)
CATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT30.26223776223776224Illumina PCR Primer Index 9 (96% over 26bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGCGTAGT30.26223776223776224No Hit
TCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAA30.26223776223776224RNA PCR Primer, Index 40 (96% over 29bp)
TCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG30.26223776223776224RNA PCR Primer, Index 47 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.26223776223776224No Hit
TCTCCAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA30.26223776223776224RNA PCR Primer, Index 47 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC30.26223776223776224RNA PCR Primer, Index 6 (96% over 25bp)
TATCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG30.26223776223776224Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGATACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG30.26223776223776224RNA PCR Primer, Index 40 (96% over 29bp)
CGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAA30.26223776223776224TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCCACCGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTT20.17482517482517482RNA PCR Primer, Index 40 (96% over 28bp)
TCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG20.17482517482517482Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT20.17482517482517482Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTTTGTGCTTCAAAAAATCGTGGCTGTTCAGCATCAGGGCGATTCGGAT20.17482517482517482No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT20.17482517482517482No Hit
GGGCATGCATTTATGACATGCCGGTGGGTTCCCAAGGAAAAATAGGCCAT20.17482517482517482No Hit
CTTATACACATCTCCGAGCCCACGAGCGCGTAGTAATCTCGTATGCCGTC20.17482517482517482No Hit
TCTCCGAGCCAACGAGACTACGCTGCACCTCGTATGCCGTCTTCTGCTTG20.17482517482517482Illumina PCR Primer Index 3 (96% over 26bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT20.17482517482517482No Hit
CTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA20.17482517482517482Illumina Paired End PCR Primer 2 (96% over 27bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA20.17482517482517482Illumina PCR Primer Index 11 (96% over 29bp)
CCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20.17482517482517482RNA PCR Primer, Index 40 (96% over 29bp)
ATACACATCTCCGAGCCCACAGACGCGTAGTAATCTCGTATGCCGTCTTC20.17482517482517482Illumina PCR Primer Index 12 (95% over 23bp)
TCTACGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG20.17482517482517482RNA PCR Primer, Index 40 (96% over 29bp)
CTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA20.17482517482517482Illumina PCR Primer Index 11 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17482517482517482No Hit
TCTCCGAGCCCACAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA20.17482517482517482Illumina PCR Primer Index 6 (96% over 28bp)
ATCCTGAAGTGCTTCGCCTTCACGCCGAGCCAGATGCTGCCGCACTATCA20.17482517482517482No Hit
ACACATCTCCGAGCACACGAGACATGCGCAGATCTCGTATGCCGTCTTCT20.17482517482517482Illumina PCR Primer Index 9 (95% over 23bp)
TCTCCGAGCCCACGAGACCGGAGCCCACGAGACCGATCAGTATCTCGTAT20.17482517482517482No Hit
CTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA20.17482517482517482Illumina PCR Primer Index 11 (96% over 29bp)
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.17482517482517482TruSeq Adapter, Index 11 (96% over 30bp)
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCATGAA20.17482517482517482Illumina PCR Primer Index 12 (96% over 27bp)
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA20.17482517482517482TruSeq Adapter, Index 12 (96% over 29bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT20.17482517482517482No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA20.17482517482517482TruSeq Adapter, Index 11 (96% over 30bp)
TCTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA20.17482517482517482RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTT20.17482517482517482TruSeq Adapter, Index 18 (96% over 27bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.17482517482517482TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT20.17482517482517482Illumina PCR Primer Index 11 (95% over 22bp)
TCTCCGAGCCCACTAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG20.17482517482517482TruSeq Adapter, Index 6 (96% over 28bp)
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA20.17482517482517482RNA PCR Primer, Index 47 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT20.17482517482517482Illumina PCR Primer Index 12 (95% over 24bp)
AGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.17482517482517482RNA PCR Primer, Index 47 (96% over 29bp)
CATCTCCGAGCCCACGATACATGCGCAGATCTCGTATGCCGTCTTCTGCT20.17482517482517482Illumina PCR Primer Index 9 (96% over 26bp)
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA20.17482517482517482Illumina PCR Primer Index 11 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCT200.001564290371.257
ACATCTC200.001564290371.258
TACACAT200.001564290371.255
ACACATC200.001564290371.256
TATACAC250.00379283257.0000043
CATCTCC250.00379283257.0000049
TTATACA250.00379283257.0000042
CTTATAC250.00379283257.0000041
ATACACA300.00781078447.54
Produced by FastQC (version 0.11.2)