FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s25.351000000d7e5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s25.351000000d7e5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA915.413444378346222Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC442.6174895895300416Illumina PCR Primer Index 10 (96% over 25bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA301.784651992861392Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG191.1302795954788816TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG181.0707911957168352TruSeq Adapter, Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT160.9518143961927424No Hit
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG160.9518143961927424Illumina PCR Primer Index 10 (96% over 28bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA150.892325996430696Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT140.8328375966686495Illumina PCR Primer Index 10 (96% over 27bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG140.8328375966686495Illumina PCR Primer Index 10 (96% over 28bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA120.7138607971445567Illumina PCR Primer Index 10 (96% over 28bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG110.6543723973825104Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG110.6543723973825104TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT110.6543723973825104TruSeq Adapter, Index 10 (95% over 21bp)
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG100.594883997620464Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT100.594883997620464RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA100.594883997620464Illumina PCR Primer Index 11 (100% over 29bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT90.5353955978584176TruSeq Adapter, Index 10 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.5353955978584176No Hit
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT90.5353955978584176Illumina PCR Primer Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG80.4759071980963712Illumina PCR Primer Index 10 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.4759071980963712No Hit
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.4759071980963712Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT80.4759071980963712Illumina PCR Primer Index 10 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.41641879833432477No Hit
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.41641879833432477Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA70.41641879833432477TruSeq Adapter, Index 11 (96% over 30bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA70.41641879833432477Illumina PCR Primer Index 11 (96% over 29bp)
GAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAAAAA60.3569303985722784RNA PCR Primer, Index 29 (96% over 27bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.3569303985722784Illumina PCR Primer Index 10 (96% over 28bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.3569303985722784Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT60.3569303985722784No Hit
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.3569303985722784RNA PCR Primer, Index 10 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.297441998810232RNA PCR Primer, Index 10 (95% over 23bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT50.297441998810232Illumina PCR Primer Index 10 (96% over 26bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA50.297441998810232Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.297441998810232Illumina PCR Primer Index 10 (95% over 23bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC50.297441998810232RNA PCR Primer, Index 11 (96% over 27bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT50.297441998810232Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT50.297441998810232No Hit
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT50.297441998810232TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGATACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.2379535990481856TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT40.2379535990481856No Hit
CTTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGT40.2379535990481856No Hit
TCTCCGAGCCCACGAGAACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.2379535990481856Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.2379535990481856RNA PCR Primer, Index 29 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT40.2379535990481856Illumina PCR Primer Index 10 (95% over 22bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA40.2379535990481856Illumina PCR Primer Index 10 (96% over 28bp)
CTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.2379535990481856Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT40.2379535990481856Illumina PCR Primer Index 10 (96% over 26bp)
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.2379535990481856RNA PCR Primer, Index 10 (96% over 28bp)
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.2379535990481856Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACAAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG30.1784651992861392Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA30.1784651992861392No Hit
TCTCCGAGCCCACTAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG30.1784651992861392Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACACACTCGCTAATCTCGTATGCCGTCTT30.1784651992861392TruSeq Adapter, Index 10 (95% over 21bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA30.1784651992861392Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGC30.1784651992861392Illumina PCR Primer Index 11 (96% over 26bp)
AGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG30.1784651992861392Illumina PCR Primer Index 10 (95% over 24bp)
CACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG30.1784651992861392Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACGAACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.1784651992861392RNA PCR Primer, Index 29 (96% over 28bp)
TCTCCGAGCCCACGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA30.1784651992861392Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGGGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG30.1784651992861392Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG30.1784651992861392TruSeq Adapter, Index 11 (96% over 29bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCT30.1784651992861392No Hit
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT30.1784651992861392Illumina PCR Primer Index 10 (96% over 26bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.1784651992861392Illumina PCR Primer Index 10 (96% over 28bp)
TACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA30.1784651992861392Illumina PCR Primer Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC30.1784651992861392No Hit
TCTCCGAGCCCCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.1784651992861392RNA PCR Primer, Index 29 (96% over 28bp)
TTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTC30.1784651992861392No Hit
CATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT30.1784651992861392RNA PCR Primer, Index 29 (96% over 27bp)
CATCTCCGACCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT20.1189767995240928Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928TruSeq Adapter, Index 10 (96% over 28bp)
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG20.1189767995240928No Hit
TCTCCGAGCCCACGAGGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCAACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCT20.1189767995240928RNA PCR Primer, Index 29 (96% over 25bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928RNA PCR Primer, Index 11 (96% over 30bp)
CTTATACACATCTACGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT20.1189767995240928No Hit
GGCTCGGAGATGTGTATAAGAGACACATCTCCGAGCCCACGAGACACTCG20.1189767995240928No Hit
TCTCCGAGGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928TruSeq Adapter, Index 11 (96% over 29bp)
TCTCCGGGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTG20.1189767995240928RNA PCR Primer, Index 29 (96% over 27bp)
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCCATGAGACACTCGCTAATCTCGTATGCCGTCTT20.1189767995240928TruSeq Adapter, Index 10 (95% over 21bp)
CATCTCCGAGCCCACCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC20.1189767995240928Illumina PCR Primer Index 10 (96% over 25bp)
ATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT20.1189767995240928Illumina PCR Primer Index 10 (95% over 21bp)
ACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC20.1189767995240928RNA PCR Primer, Index 29 (96% over 25bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA20.1189767995240928No Hit
CCCGAATACTGTGCTTTTCCAACGGGCTTAAAACACAGCGCACCTGGAGA20.1189767995240928No Hit
CATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928Illumina PCR Primer Index 11 (96% over 30bp)
CTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928RNA PCR Primer, Index 10 (96% over 28bp)
ATACACATCTCCGACCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC20.1189767995240928TruSeq Adapter, Index 10 (95% over 22bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT20.1189767995240928TruSeq Adapter, Index 11 (95% over 23bp)
TCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.1189767995240928Illumina PCR Primer Index 10 (96% over 27bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1189767995240928No Hit
ATACACATCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTT20.1189767995240928TruSeq Adapter, Index 11 (95% over 22bp)
ATACACATCTCCGAGCCAACGAGACACTCGCTAACCTCGTATGCCGTCTT20.1189767995240928No Hit
TCTCCGAGCCTACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928TruSeq Adapter, Index 11 (96% over 30bp)
AAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT20.1189767995240928Illumina PCR Primer Index 10 (95% over 23bp)
GGCTCGGAGATGTGTATAAGAGACAGCATCTCCGAGCCCACGAGACGGAG20.1189767995240928No Hit
CATCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT20.1189767995240928TruSeq Adapter, Index 11 (96% over 28bp)
GCACCCCAGCACAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTA20.1189767995240928No Hit
TCTCCGAAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.1189767995240928Illumina PCR Primer Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT20.1189767995240928No Hit
TCTCCGACCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928RNA PCR Primer, Index 29 (96% over 28bp)
CTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928TruSeq Adapter, Index 10 (96% over 28bp)
ACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC20.1189767995240928RNA PCR Primer, Index 29 (96% over 25bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC20.1189767995240928No Hit
CATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT20.1189767995240928TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928Illumina PCR Primer Index 10 (96% over 28bp)
CCCCTGGGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC20.1189767995240928No Hit
CATCTCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTT20.1189767995240928TruSeq Adapter, Index 12 (96% over 25bp)
TCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGT20.1189767995240928No Hit
CTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.1189767995240928TruSeq Adapter, Index 10 (96% over 28bp)
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1189767995240928No Hit
CTTATACACATCTCCGAGCCCACTATACACTCGCTAATCTCGTATGCCGT20.1189767995240928No Hit
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20.1189767995240928Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCT20.1189767995240928Illumina PCR Primer Index 11 (96% over 25bp)
TCTCCAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA20.1189767995240928Illumina PCR Primer Index 11 (96% over 29bp)
GATCGGAAGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGC20.1189767995240928RNA PCR Primer, Index 12 (95% over 23bp)
TCTCCGAGCCCTCGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.1189767995240928TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCATGAGACACTCGCTAATCTCGTATGCCGT20.1189767995240928No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAACC150.00944322247.556-57
TCTGCTT1100.00744344312.95454642-43
GTCTTCT1100.00744344312.95454638-39
CTTCTGC1100.00744344312.95454640-41
AAAAAAA8502.8297527E-65.30882352-53
Produced by FastQC (version 0.11.2)