FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s24.351000000d7e35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s24.351000000d7e35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG21.342281879194631TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT21.342281879194631No Hit
ATCCTTTTCCACTTAACATATATTTTGGGACCTTAGCTGGTGGTCTGGGC10.6711409395973155No Hit
ATTGTATATTGTCTATTTTGTCTATTGTATATTTCTGTCTCTTATACACA10.6711409395973155No Hit
GATCACGGCTTCTGGGGCAGCACATCGTACTACAACTGGCGCACCGTGTA10.6711409395973155No Hit
GATTAGGTGCCCGTACCGCAAACCGACACAGGTAGTCAAGATTAGAATTC10.6711409395973155No Hit
GTCGAGAAAAGCCTCTAGATAGAGATTAGGTGCCCGTACCGCAAACCGAC10.6711409395973155No Hit
GGGTTGAGTATCCTCGACGACTCGCCTCCATGGATTGCTAACGGTTGCCG10.6711409395973155No Hit
GTCTTATGACAAGTGGGTTTCCCCATTCAGATATCGCCGGATCACAGGAT10.6711409395973155No Hit
ACAGAGGTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAA10.6711409395973155No Hit
CCTAAGCCGAAGGCAAAGGCTGCCCCAGCTCCCAGCAAGGTGGTCAAGAA10.6711409395973155No Hit
CAAATTTCCTACGCCCACGACGGATAGGGACCGAACTGTCTCACGACGTT10.6711409395973155No Hit
GCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTT10.6711409395973155No Hit
AGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTG10.6711409395973155No Hit
GCAGAGACCTGTGTTTTTGATAAACAGTCGCTTGGGCCTATTCACTGCGG10.6711409395973155No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA10.6711409395973155No Hit
TCCCTAATGAGAACGGAGAGCACACGTCTCCCTAATGAGAACGGAGAGCA10.6711409395973155No Hit
ACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAA10.6711409395973155No Hit
ATCTAACGCGGGGCAGACCCGGAGAACTGAAACATCTTAGTACCCGGAGG10.6711409395973155No Hit
GCTTAATAGAGGCCAAAGCGGTCTGGAAACGTACGGATTGTTCAGTAACT10.6711409395973155No Hit
CCAGGATGCGATGAGCCGACATCGAGGTGCCAAGCTTCCCCGTCGATGTG10.6711409395973155No Hit
CAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAA10.6711409395973155No Hit
TTATAATGCCATTCCCCGCGCCAGGACTCCCCCACATCTCCTTCGAATCT10.6711409395973155No Hit
ACCTTCCAGCACCGGGCAGGCATCACACCCTATACGCTGTCTCTTATACA10.6711409395973155No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10.6711409395973155No Hit
GCCAAGGACACCAAGAAGCCGGCCACCAACAGTTGCCAGCCTGTCTCTTA10.6711409395973155No Hit
CTCTTCCGATCTGCAGCGGGGGCACAGCTTGTACAGGATAGGTAGGAGCC10.6711409395973155No Hit
GGCTTGACATTTCCCTGAAATAGTCCACAGTAACGTTCGTATCTGCAAGA10.6711409395973155No Hit
GCAGTCCATTGCCGAATCGGAATACGCCAAGAACAAGGAGAACCGCAAAA10.6711409395973155No Hit
GCACAAGTTCAACTACGAACGTTTTAAACGCAACAACTTTAATATACGCT10.6711409395973155No Hit
CTTATAACCGAGTTGGGAAATCTCATCTTGAGGGGGGCTTCATGCTTAGA10.6711409395973155No Hit
CCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGGGCTAGAAGAGCATG10.6711409395973155No Hit
AAATTGATCATTGTCGAGGGTTGCCAGCGCATGGGAGCTATTGTGGCCGT10.6711409395973155No Hit
ACACCGAACTGCCCAGGAGTCCAGTTGGATACACGATCCACCTCACATAT10.6711409395973155No Hit
GGCCAAATCGATAACAGAAACGAGAACCACAAATTCTACGGCGGCAACCA10.6711409395973155No Hit
CTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGA10.6711409395973155No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT10.6711409395973155TruSeq Adapter, Index 12 (95% over 22bp)
CAATACCGCCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCT10.6711409395973155No Hit
AGTTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCA10.6711409395973155No Hit
GGGAAAGGGTCAGGCCGCGGCGTCCGCGGCCCGGTTGAGTTGTAGCCGCA10.6711409395973155No Hit
AACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAG10.6711409395973155No Hit
GAACTCTGACAGCGATAGAGACACCAAGCACAGGATGCGCAATCCTTTGG10.6711409395973155No Hit
CCCCGTGTTGAATCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCT10.6711409395973155No Hit
GGGTGCTTTTTAGGGTTCACGCCCAGAAGAGCCGCAGTGAATAGGCCCAA10.6711409395973155No Hit
GGATTTGCAGACCCATCAGGGCCACCACCAGGGCCACCAGCAAGGCCGCA10.6711409395973155No Hit
CTTTTGCTGGGCAACTCGCCGCTTTAATAAGCACTTGGGCAGCAGGAGGT10.6711409395973155No Hit
GTTATAACCTCACACTCAATCTTTTATCACGAAGTCATGATTGAATCGCG10.6711409395973155No Hit
GAACTCACTAGAGGCTTTTCTCGGCAGCATAGGATCACTAAAGCACAGCT10.6711409395973155No Hit
CGACAACGGCGACGATGCCATCCTGCTCGCGCGCGAGCACCGCCCGCAGC10.6711409395973155No Hit
GTTTATCACCGCCTTCGTCATCCCTTGCCTGGTTATCATTGCCTGCTATG10.6711409395973155No Hit
TCTCGATGTCGTTGCCCGACAGATTCTCGTATTCGAGGAAGATCTGGCGC10.6711409395973155No Hit
CAAGCAACTTATCTGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCA10.6711409395973155No Hit
GCTGCAAGACTTTGTGGTGGAGCAGATCCACATGCATTGGTGGTCGGAGC10.6711409395973155No Hit
ATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAGACTCATCA10.6711409395973155No Hit
CCCAAGGCCCGGTGGGGTCGCCGGCGGTTGTAGTGGTCAAGGTAGGCCTC10.6711409395973155No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC10.6711409395973155No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGT10.6711409395973155No Hit
GGAGGAACATATGAAAAGATACAAGTATTTAATCACATATAAGAACTCCA10.6711409395973155No Hit
CCTTTCGTCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGA10.6711409395973155No Hit
CTATGATTTCACCGAGTCTCTCGTTGAGACAGCTGTCTCTTATACACATC10.6711409395973155No Hit
CTTCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACTAGCGAT10.6711409395973155No Hit
CTGATACTTAGACTCGTAGTGTTTCATATTAGTTTTTCATAACTCAAGCC10.6711409395973155No Hit
CCCCATCAATTAACCTTCCAGCACCGGGCAGGCATCACACCCTATACGTC10.6711409395973155No Hit
CTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGA10.6711409395973155No Hit
CTCTTCGCCCTTTGCGGTAAACGAGCAAAGTTCGATGACCGGCGGGGAAC10.6711409395973155No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
CTTCTGTATTCTCTTCCTCAGTACTTCTGTTTAGTTATTTTCTGTGGATT10.6711409395973155No Hit
ATCCTGATAAACTCCGAATGCCATCCATGCCAGTCCGGGAGTCACACTGC10.6711409395973155No Hit
GTGCTGAGCTGCGAGACCTTTGGCATCCAGCCCGGTTCGGCCTTGACTGA10.6711409395973155No Hit
CCTCAGTGAGTTTGTCTACAACAACTACTAGGATGACAGGATGGTTTTGC10.6711409395973155No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA10.6711409395973155No Hit
CCTAAATACTACCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGG10.6711409395973155No Hit
TTCTTGACCCAGACTGTTTGTTTCGTTCTGCATTTTTCGGTCGTAGCCAA10.6711409395973155No Hit
GCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGACTGAAGTC10.6711409395973155No Hit
GTATTGTGCATATTTAGCAATTGTAGTATGAGATCTATTACTTTCACAAT10.6711409395973155No Hit
CTTTTCAGGCCTGATCACCCCTGTGGCCACTCCGATCACCCCTATGGCTG10.6711409395973155No Hit
GTGCTCTTCCGATCTGGCGTGGGCTTCCTGTCTCTTATACACATCTCCGA10.6711409395973155No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG10.6711409395973155No Hit
GTCTTCACAAAATCCAAGAATTTCACCTCTAGCATGTGAGTACTAATGCT10.6711409395973155No Hit
ATTTGTTGCTCTCCATGGTTTGTAGGTGTTGTTTGCGGGTGTTCCTGGTT10.6711409395973155No Hit
ATGTAATGGAGAGTAAGGGAGTTGAATAGAATCTATCCGAATGTAATGGA10.6711409395973155No Hit
TGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACT10.6711409395973155No Hit
CAACGTGAGAGTGTCAAAAACGATAAACCAACCATCAGCATGAGCCTGTC10.6711409395973155No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG10.6711409395973155No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT10.6711409395973155No Hit
ACATCGACCAGATGTCGTACATAAAGTAAGCAGTGCCGAACCAACCGTAC10.6711409395973155No Hit
TCTCCGAGCCCACGAGACGCGTAGTAACCTCGTATGCCGTCTTCTGCTTG10.6711409395973155Illumina PCR Primer Index 12 (96% over 27bp)
GCCAAAGCGGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGAT10.6711409395973155No Hit
ACACTAAAGAGCACAATCCCTGCGCCAAGGATGAAAGCCAACACCTTAGA10.6711409395973155No Hit
GACTACCTGTGTCGGTTTGCGGTACGGGCACCTAATCTCTATCTAGAGGC10.6711409395973155No Hit
CAGCAGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTG10.6711409395973155No Hit
GCACGCAGTTCCTCGATTTTCCGGTGGCGCTCCTCATTTTTGGCGATCGC10.6711409395973155No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAGAGGCCACCCCCTTTTTT10.6711409395973155No Hit
CTATAGCAAGACAAGGGACGAAACACTGGAATCTGCGCCTAGGAGGCCTG10.6711409395973155No Hit
GGTAGATGGCGTTCACCGGGCAAGCGGGCACGCAGGCCCCGCAGTCAATG10.6711409395973155No Hit
CACGAAGAAGGTGGCCAGCATCAGCAGACCGTTGGCGATGTACACACGTG10.6711409395973155No Hit
GTATACAATTTTCTGTAATGGTTGCCCCGCCCTAGATCGGAAGAGCACAC10.6711409395973155No Hit
GCATAGCCCGGAAAAAATAAACGTGGCAACGAGGGGATATCGAAGGGGAA10.6711409395973155No Hit
GCTTCATCCCGTCATAGTCGAATGTCGTCTCATCCAGACGCACGGAAACG10.6711409395973155No Hit
GACTTACGCATTGCAAATGAAGCAAATGGAAATTTACATATCAATCGCCG10.6711409395973155No Hit
CTTCCGATCTGCGTGGGGCTCGATCGGCACCTTACGAGAAATCAAAGTTT10.6711409395973155No Hit
GAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATCGTTACGG10.6711409395973155No Hit
TCCTTTAGGTGTATCATTAGGTTGCTTATTTAAAAATCTTTCTACTTTTT10.6711409395973155No Hit
ATTTCACACTGCCGAGAAAAGCCTCTAGATAGAAACAGGTGCCCGTACCG10.6711409395973155No Hit
CGCCGGGTCTCGAGTCGTTGATTTCACACTGCCGAGAAAAGCCTCTAGAT10.6711409395973155No Hit
CTCTTGGGAGACGTGTGCTCTTCCGATCTCTCTTGGGAGACGTGTGCTCT10.6711409395973155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGTGGACTGCGCCGGTTGGGCCATCCAAGTGCTGTGGAGACTGTAAAA10.6711409395973155No Hit
CTTCTAAGACAGCCGTGGGGGAGGGAGCTCGCTGCAAGCGACTACTCAGC10.6711409395973155No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAAACCC10.6711409395973155No Hit
TAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAG10.6711409395973155No Hit
CCACAAGTAGCGAATGGGCAGCTTAGAAACGCAGCTTCTGGAAGAACCAC10.6711409395973155No Hit
GTCTTGATCGGAGCCCTCTTCATTTAGGGAAGTATGCTCCGTAGATGGCT10.6711409395973155No Hit
CCGTTATAGTTACGGCCGCCGTTTACTGGGGCTTCGATTCGTAGCTTCGC10.6711409395973155No Hit
CAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTCTGTCT10.6711409395973155No Hit
GGTGGACACCTCCCGCATCCACATCTTCGACCCGAAGACCGGCGAGAACC10.6711409395973155No Hit
CTCCTTTGCTTTTGCTTTCCTTTTAAATTCGAAGATTTCCTTTTTCCTTT10.6711409395973155No Hit
GGGCGAAGGTTAGGGTATCGAAAACGATTAGATACCGTTGTAGTCTTAAC10.6711409395973155No Hit
CCTACGGACAGATCGGCATGATCCAGGCCGCCGCCGGCTTCTTTGTGTAC10.6711409395973155No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT10.6711409395973155TruSeq Adapter, Index 12 (96% over 28bp)
AAAACCAAGCGATAGCAAGATCGTTATATTGGTTGAACTCTAGATAACAT10.6711409395973155No Hit
GTCTGGGGGACCTCATCGCCTTCATGGCCAATAAGGACGGTGCCAACTAA10.6711409395973155No Hit
CCTATTAGTGGTTGAACAGCATCGGACTCAGATAGTAATCCACGCTCTTT10.6711409395973155No Hit
TGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACG10.6711409395973155No Hit
ACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACCAC10.6711409395973155No Hit
GAATTATCACCCATGTTCTGACTCCCAAGTTAAATTGATTGGCATTCGGA10.6711409395973155No Hit
CTTCCGATCTGGTTTGGGCTCTTCCCGTTTCGCTCGCCGCTACTCAGGGA10.6711409395973155No Hit
CTTTCGTCCCTGCTCGACTTGTCTGTCTCGCAGTCAAGCTCCCTTCTGCC10.6711409395973155No Hit
GATTATCCATGTAGAATAGCAATTTCTTCCACGACGCCGCACAGCAGAGC10.6711409395973155No Hit
AAATACTTGTATCTTTTCATATGTTCCACCTATTTAAAAACCTGCATTAG10.6711409395973155No Hit
GCAATATTAGCAGCATTTCCTCAGTAGTCCTCAGATCGCAGGCTCCTGCC10.6711409395973155No Hit
AGTAATATCTCTGAGCAGGAGGAGTCATGGCAAGGACAGACTTTAAATTG10.6711409395973155No Hit
GTACTGGGAGACGTGTGCTCTTCCGATCTGTACTGGGAGACGTGTGCTCT10.6711409395973155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCGAAGCTACGGGTATCACGTAAGTGATGCGGTAGAGGAGCGTTCTGT10.6711409395973155No Hit
TCAGCAATCTTAAACTTCTTAGACGAATCACCAGAACGGAAAACATCCTT10.6711409395973155No Hit
GCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAA10.6711409395973155No Hit
ATCTTTAAAAGGGCAACAATAGACAGACAGTTAACTGCTCAGCAGAAACA10.6711409395973155No Hit
TCGTGTATCCGTTGAAGCTGCAGAGTGATCTAGTGGTGGAGGGTATGCGA10.6711409395973155No Hit
TTTCTCGGCAGTGTGAAATCAACGACTCGAGGAAACAATTCCCTCTCCCC10.6711409395973155No Hit
AGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTT10.6711409395973155No Hit
AGAACGAGAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATACTGT10.6711409395973155No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGT10.6711409395973155No Hit
GCCCTAGCAGCTTCATCCCTCAATGCATTTGCAGCTACATTCACAGCATC10.6711409395973155No Hit
AATGAGTTCAATGCGTTATCGTCTGGATGGTTTCCTGAAGCTTATGCCCA10.6711409395973155No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT10.6711409395973155RNA PCR Primer, Index 12 (95% over 21bp)
GCTGGACTAGCGCGTCCAAGCTAGGAGCTAGGTCTGTATCTTATACACAT10.6711409395973155No Hit
CTTCCGATCTAGGCCGGGCTTCGGGTGTTTCAGACTCTCGTGGTGTGACG10.6711409395973155No Hit
CCCTATACATCACCTTACGGTTTAGCAGAGACCTGTGTTTTTGATAAACA10.6711409395973155No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers