FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s22.351000000d7df0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s22.351000000d7df0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4849694
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT742821.5316842670898412No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT512391.0565408869095658TruSeq Adapter, Index 12 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482710.9953411493591142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA327620.6755477768288061No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327510.6753209583944885No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309190.6375453791517568No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT264950.5463231288407063RNA PCR Primer, Index 12 (95% over 24bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG213830.44091441645596613TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC165610.34148546279414743No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT90340.18627979414783694No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT86230.17780503264742065TruSeq Adapter, Index 12 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT84020.17324804410340117RNA PCR Primer, Index 12 (95% over 21bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT66060.13621477973661844No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA64900.13382287624744985No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG60640.12503881688205484No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT53070.10942958462946321No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA52760.10879036904184057No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT52560.10837797188853564No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51670.10654280455632871No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG50330.10377974362918568TruSeq Adapter, Index 12 (96% over 25bp)
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG48650.10031560754142427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT252000.030.5916926
ATCTTTT261800.029.5179067
CGATCTT310900.027.072515
TCTTTTT283900.026.7014688
CTTTTTT299050.025.3011119
TTCCGAT636750.022.8861082
TATATGC35750.022.8527223
CCGATCT633700.022.8388544
TCCGATC646850.022.4847033
CTTCCGA659400.022.4019361
TATGCTT35550.022.3132295
ATGCTTG34050.022.1802046
CTTGTCT37050.021.4089769
CTAGGAC51050.020.8419253
ATCTAGG51750.020.5793131
ATATGCT37900.020.5537094
TCTAGGA54800.020.4558262
TAGGACT52300.020.434614
ATAATAC6950.019.8197773
GTCCTAG37000.019.788471
Produced by FastQC (version 0.11.2)