FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s21.351000000d7dc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s21.351000000d7dc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA462.017543859649123Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311.3596491228070176No Hit
GAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAA301.3157894736842104RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG281.2280701754385965TruSeq Adapter, Index 7 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC271.1842105263157896RNA PCR Primer, Index 29 (96% over 27bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA170.7456140350877193RNA PCR Primer, Index 42 (96% over 28bp)
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA160.7017543859649122RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG140.6140350877192983RNA PCR Primer, Index 29 (96% over 30bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA130.5701754385964912Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA130.5701754385964912Illumina Paired End PCR Primer 2 (96% over 29bp)
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA110.4824561403508772Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG100.43859649122807015TruSeq Adapter, Index 5 (96% over 28bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA100.43859649122807015RNA PCR Primer, Index 29 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.39473684210526316No Hit
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA90.39473684210526316Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAA90.39473684210526316TruSeq Adapter, Index 7 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC80.3508771929824561Illumina Paired End PCR Primer 2 (96% over 26bp)
AGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA70.30701754385964913TruSeq Adapter, Index 7 (96% over 28bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.30701754385964913Illumina PCR Primer Index 10 (96% over 29bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA70.30701754385964913RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT70.30701754385964913Illumina PCR Primer Index 10 (95% over 22bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.30701754385964913TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.2631578947368421TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.2631578947368421RNA PCR Primer, Index 29 (96% over 30bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG60.2631578947368421No Hit
TCTCCGAGCCAACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG60.2631578947368421Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG60.2631578947368421TruSeq Adapter, Index 7 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT50.21929824561403508RNA PCR Primer, Index 10 (95% over 24bp)
TCTCAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG50.21929824561403508TruSeq Adapter, Index 5 (96% over 28bp)
CATCTCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT50.21929824561403508TruSeq Adapter, Index 7 (96% over 27bp)
CATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT50.21929824561403508TruSeq Adapter, Index 10 (96% over 27bp)
CTTATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGT40.17543859649122806No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC40.17543859649122806Illumina PCR Primer Index 10 (96% over 25bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17543859649122806No Hit
GATCGGAAGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGC40.17543859649122806Illumina PCR Primer Index 12 (95% over 23bp)
AAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC40.17543859649122806No Hit
CTTATACACATCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT40.17543859649122806No Hit
TCTCCGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGTCTTCTGCTTG40.17543859649122806Illumina PCR Primer Index 12 (96% over 26bp)
TCTCCGAGCCCACGATACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG40.17543859649122806Illumina PCR Primer Index 7 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTGAGC40.17543859649122806No Hit
CCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAA40.17543859649122806RNA PCR Primer, Index 7 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17543859649122806No Hit
CTCCGAGCCAACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA30.13157894736842105Illumina PCR Primer Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGC30.13157894736842105RNA PCR Primer, Index 7 (96% over 25bp)
TCTCCTAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105Illumina Paired End PCR Primer 2 (96% over 29bp)
TCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13157894736842105No Hit
CATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT30.13157894736842105Illumina PCR Primer Index 10 (96% over 27bp)
TCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105Illumina PCR Primer Index 7 (96% over 28bp)
TCTCCAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.13157894736842105RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCTAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCCACTAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105RNA PCR Primer, Index 29 (96% over 30bp)
CCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAA30.13157894736842105RNA PCR Primer, Index 5 (96% over 28bp)
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.13157894736842105RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA30.13157894736842105Illumina PCR Primer Index 7 (96% over 28bp)
ATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT30.13157894736842105TruSeq Adapter, Index 7 (95% over 21bp)
TCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTGAA30.13157894736842105Illumina PCR Primer Index 7 (96% over 26bp)
TCTCCGAGCCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT30.13157894736842105RNA PCR Primer, Index 29 (96% over 29bp)
CATCTCAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT30.13157894736842105Illumina Paired End PCR Primer 2 (96% over 27bp)
CTCCGAGCCCACTAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA30.13157894736842105RNA PCR Primer, Index 7 (96% over 28bp)
TCTCCGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCCACGATACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG30.13157894736842105Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT30.13157894736842105No Hit
CTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA30.13157894736842105RNA PCR Primer, Index 10 (96% over 29bp)
CTACGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA30.13157894736842105RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGT30.13157894736842105No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT300.003550648731.66666814-15
ACTGAGC350.00754846727.14285918-19
ACACTGA350.00754846727.14285916-17
AGCGATC350.00754846727.14285922-23
TGAGCGA350.00754846727.14285920-21
GATCGGA601.3709965E-423.7510-11
AGATCGG600.004077715319.79166810-11
TCGGAAG756.212882E-418.9999983
ATCGGAA650.00648170318.26923212-13
AAGAGCC850.001442305616.76470616-17
GGAAGAG850.001442305616.7647065
TCTGCTT1501.6286938E-514.24999942-43
TGCTTGA1501.6286938E-514.24999944-45
CTTGAAA1501.6286938E-514.24999946-47
CTTCTGC1501.6286938E-514.24999940-41
CTCGTAT1552.2224936E-513.79032228-29
TGCCGTC1603.0013705E-513.35937634-35
TATGCCG1603.0013705E-513.35937632-33
GTCTTCT1603.0013705E-513.35937638-39
CGTATGC1603.0013705E-513.35937630-31
Produced by FastQC (version 0.11.2)