FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s2.351000000d7b52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s2.351000000d7b52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7136
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG1421.9899103139013452RNA PCR Primer, Index 47 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT1231.7236547085201794No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA751.0510089686098656RNA PCR Primer, Index 47 (96% over 29bp)
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA731.022982062780269Illumina PCR Primer Index 9 (100% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA721.0089686098654709No Hit
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA710.9949551569506726Illumina PCR Primer Index 5 (96% over 28bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA660.9248878923766817RNA PCR Primer, Index 40 (96% over 29bp)
TCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAA640.8968609865470852TruSeq Adapter, Index 9 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT610.8548206278026906Illumina PCR Primer Index 10 (95% over 21bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA390.54652466367713Illumina PCR Primer Index 11 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC360.5044843049327354RNA PCR Primer, Index 12 (96% over 26bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA360.5044843049327354Illumina PCR Primer Index 11 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT350.4904708520179373Illumina PCR Primer Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG340.476457399103139TruSeq Adapter, Index 12 (96% over 29bp)
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA320.4484304932735426TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG300.4204035874439462Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG250.3503363228699552Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT240.336322869955157No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG240.336322869955157TruSeq Adapter, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC220.30829596412556054RNA PCR Primer, Index 47 (96% over 26bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA190.2662556053811659TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCCACGATACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG190.2662556053811659Illumina PCR Primer Index 9 (96% over 28bp)
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA190.2662556053811659Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT180.2522421524663677RNA PCR Primer, Index 47 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT160.2242152466367713Illumina PCR Primer Index 10 (96% over 26bp)
TCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG160.2242152466367713TruSeq Adapter, Index 9 (96% over 28bp)
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA150.2102017937219731RNA PCR Primer, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT150.2102017937219731No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGA140.1961883408071749Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTT140.1961883408071749Illumina PCR Primer Index 12 (95% over 22bp)
AGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA140.1961883408071749RNA PCR Primer, Index 47 (96% over 29bp)
CCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAA140.1961883408071749RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACTAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG140.1961883408071749TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCAACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG140.1961883408071749RNA PCR Primer, Index 40 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT140.1961883408071749TruSeq Adapter, Index 12 (95% over 22bp)
TCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG130.18217488789237668TruSeq Adapter, Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTGATCTCGTATGCCGT130.18217488789237668No Hit
TCTCCGAGCCCACTAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG130.18217488789237668RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCTAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG130.18217488789237668RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACGATACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG130.18217488789237668TruSeq Adapter, Index 11 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACATGCGCA120.1681614349775785No Hit
TCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG120.1681614349775785TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC120.1681614349775785No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT120.1681614349775785RNA PCR Primer, Index 47 (95% over 24bp)
TCTCCTAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG120.1681614349775785Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTT120.1681614349775785Illumina PCR Primer Index 9 (95% over 21bp)
CCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAA110.15414798206278027RNA PCR Primer, Index 12 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.15414798206278027No Hit
TCTCCGAGCCCACGAGACATGCGCAGACCTCGTATGCCGTCTTCTGCTTG110.15414798206278027Illumina PCR Primer Index 9 (96% over 27bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA110.15414798206278027RNA PCR Primer, Index 11 (96% over 30bp)
ACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT110.15414798206278027Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTCCGAGCCCACGAGACATGCGCAGAACTCGTATGCCGTCTTCTGCTTG100.14013452914798205Illumina PCR Primer Index 9 (96% over 27bp)
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT100.14013452914798205No Hit
TCTCCGAGCCCACGATACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG100.14013452914798205Illumina PCR Primer Index 12 (96% over 29bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC100.14013452914798205RNA PCR Primer, Index 6 (96% over 25bp)
TCTCCTAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG100.14013452914798205TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT100.14013452914798205No Hit
TCTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA100.14013452914798205RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCCCACTAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG100.14013452914798205TruSeq Adapter, Index 6 (96% over 28bp)
TCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG100.14013452914798205TruSeq Adapter, Index 12 (96% over 29bp)
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG100.14013452914798205Illumina PCR Primer Index 10 (95% over 24bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA100.14013452914798205RNA PCR Primer, Index 11 (96% over 30bp)
ATACACATCTACGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT100.14013452914798205TruSeq Adapter, Index 9 (95% over 21bp)
TCTCCGAGCACACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG100.14013452914798205RNA PCR Primer, Index 40 (96% over 29bp)
TATCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG100.14013452914798205RNA PCR Primer, Index 47 (96% over 29bp)
TCTCCGAGCACACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG90.12612107623318386Illumina PCR Primer Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGT90.12612107623318386No Hit
ATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT90.12612107623318386TruSeq Adapter, Index 9 (95% over 21bp)
CATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT90.12612107623318386Illumina PCR Primer Index 9 (96% over 26bp)
CTTATACACATCTCAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT90.12612107623318386No Hit
CATCTCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT90.12612107623318386RNA PCR Primer, Index 47 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGT90.12612107623318386No Hit
TCTCCGAGCCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT90.12612107623318386RNA PCR Primer, Index 47 (96% over 28bp)
AGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA90.12612107623318386TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCAACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG90.12612107623318386TruSeq Adapter, Index 11 (96% over 29bp)
TCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG80.11210762331838565Illumina PCR Primer Index 12 (96% over 29bp)
TCTCCGAGCCCACTAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG80.11210762331838565Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT80.11210762331838565TruSeq Adapter, Index 12 (96% over 28bp)
TCTCCTAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG80.11210762331838565TruSeq Adapter, Index 6 (96% over 28bp)
TCCGAGCCCACGAGACATGCGCAGAACTCGTATGCCGTCTTCTGCTTGAA80.11210762331838565Illumina PCR Primer Index 9 (96% over 27bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG80.11210762331838565TruSeq Adapter, Index 11 (96% over 30bp)
ACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT80.11210762331838565Illumina PCR Primer Index 12 (95% over 24bp)
TCTCCGAGCCCACGATACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG80.11210762331838565RNA PCR Primer, Index 40 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCT80.11210762331838565Illumina PCR Primer Index 9 (95% over 23bp)
TCTCCGAGCCAACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG80.11210762331838565Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT80.11210762331838565No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT156.348017E-495.02
CTTCCGA156.348017E-495.01
CATCTCA200.001989950771.259
CCGATCT200.001989950771.254
CGATCTT200.001989950771.255
CTTATAC1400.050.8928531
TCCGATC300.00991080647.53
TTATACA1600.044.5312542
TATACAC1700.041.9117663
TACACAT2200.032.3863645
TCGCTAA300.003822093831.66666830-31
ATACACA2300.030.9782624
CACATCT2450.029.081637
TCCGAGC2650.028.6792473
TCTCCGA2550.027.9411751
CGCTAAT350.00812028827.14285532-33
CTCGCTA350.00812028827.14285530-31
CTCCGAG2650.026.8867932
ACTCGCT458.518177E-426.38889128-29
ACACATC2750.025.9090926
Produced by FastQC (version 0.11.2)