FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s19.351000000d7d79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s19.351000000d7d79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA1133.4779932286857496Illumina PCR Primer Index 11 (100% over 29bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA521.600492459218221TruSeq Adapter, Index 11 (96% over 30bp)
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA451.3850415512465373Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG320.9849184364419821TruSeq Adapter, Index 11 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.9233610341643583No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA250.7694675284702985RNA PCR Primer, Index 11 (96% over 30bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA230.7079101261926747RNA PCR Primer, Index 11 (96% over 30bp)
TCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG210.6463527239150507TruSeq Adapter, Index 11 (96% over 30bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC190.5847953216374269RNA PCR Primer, Index 29 (96% over 27bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC180.554016620498615RNA PCR Primer, Index 11 (96% over 27bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA180.554016620498615TruSeq Adapter, Index 11 (96% over 30bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA180.554016620498615RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT170.523237919359803No Hit
TCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG160.49245921822099104Illumina PCR Primer Index 11 (96% over 30bp)
TCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG150.46168051708217916TruSeq Adapter, Index 11 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA150.46168051708217916No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC150.46168051708217916RNA PCR Primer, Index 41 (96% over 26bp)
GAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAA140.43090181594336713RNA PCR Primer, Index 40 (96% over 29bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA140.43090181594336713Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG120.3693444136657433Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG120.3693444136657433RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTT110.3385657125269314Illumina PCR Primer Index 11 (95% over 23bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG110.3385657125269314TruSeq Adapter, Index 11 (96% over 30bp)
TATCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG110.3385657125269314RNA PCR Primer, Index 11 (96% over 30bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.3385657125269314No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.3077870113881194No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA100.3077870113881194RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT100.3077870113881194No Hit
ACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT90.2770083102493075Illumina PCR Primer Index 11 (100% over 24bp)
ATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT90.2770083102493075TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT90.2770083102493075No Hit
CGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA90.2770083102493075TruSeq Adapter, Index 11 (96% over 30bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG80.24622960911049552TruSeq Adapter, Index 10 (96% over 29bp)
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT80.24622960911049552TruSeq Adapter, Index 11 (96% over 29bp)
CTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA80.24622960911049552Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG80.24622960911049552TruSeq Adapter, Index 10 (96% over 29bp)
ACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGTCTTCT80.24622960911049552Illumina PCR Primer Index 11 (96% over 25bp)
TCCGAGCCCACGAGACGGAGCTACACCTCGTATGCCGTCTTCTGCTTGAA70.21545090797168356Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA70.21545090797168356RNA PCR Primer, Index 11 (96% over 30bp)
ATACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT70.21545090797168356TruSeq Adapter, Index 11 (95% over 23bp)
TCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.21545090797168356TruSeq Adapter, Index 10 (96% over 29bp)
TCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.18467220683287164Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT60.18467220683287164Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGAGAGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA60.18467220683287164Illumina PCR Primer Index 11 (100% over 29bp)
TCTCCGAACCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG60.18467220683287164Illumina PCR Primer Index 11 (96% over 30bp)
TCTCCTAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG60.18467220683287164RNA PCR Primer, Index 29 (96% over 30bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA60.18467220683287164RNA PCR Primer, Index 29 (96% over 30bp)
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG60.18467220683287164TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT60.18467220683287164No Hit
CATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT60.18467220683287164TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT50.1538935056940597Illumina PCR Primer Index 10 (95% over 24bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG50.1538935056940597Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCACACGAGACGGAGCTACATCTCGTATGCCGTCTT50.1538935056940597Illumina PCR Primer Index 11 (95% over 23bp)
GATCGGAAGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGC50.1538935056940597RNA PCR Primer, Index 6 (96% over 25bp)
CTTATACACATCTCCGAGCCCACAGACGGAGCTACATCTCGTATGCCGTC50.1538935056940597Illumina PCR Primer Index 11 (95% over 21bp)
CATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT50.1538935056940597TruSeq Adapter, Index 10 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGCGGAGCTACATCTCGTATGCCGTCTTC50.1538935056940597Illumina PCR Primer Index 11 (95% over 24bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG50.1538935056940597Illumina PCR Primer Index 10 (96% over 29bp)
TATCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.12311480455524776RNA PCR Primer, Index 29 (96% over 30bp)
CTCCTAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776RNA PCR Primer, Index 11 (96% over 30bp)
ACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT40.12311480455524776Illumina PCR Primer Index 11 (100% over 24bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG40.12311480455524776RNA PCR Primer, Index 29 (96% over 30bp)
TCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776TruSeq Adapter, Index 11 (96% over 30bp)
CTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCAACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.12311480455524776Illumina PCR Primer Index 3 (96% over 28bp)
CTCCGAGCCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.12311480455524776Illumina PCR Primer Index 11 (96% over 30bp)
CTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAACGGAGCTACATCTCGTATGCCGTC40.12311480455524776Illumina PCR Primer Index 11 (95% over 21bp)
TCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGATACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG40.12311480455524776RNA PCR Primer, Index 40 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTT40.12311480455524776TruSeq Adapter, Index 10 (95% over 22bp)
ATACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT40.12311480455524776Illumina PCR Primer Index 10 (95% over 22bp)
CATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.12311480455524776TruSeq Adapter, Index 11 (96% over 28bp)
AGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA40.12311480455524776TruSeq Adapter, Index 6 (96% over 28bp)
CACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG40.12311480455524776TruSeq Adapter, Index 11 (96% over 26bp)
GCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA40.12311480455524776Illumina PCR Primer Index 11 (100% over 29bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCT40.12311480455524776No Hit
TCCGAGCCCACGAGACGGAGCTACAACTCGTATGCCGTCTTCTGCTTGAA40.12311480455524776Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.12311480455524776TruSeq Adapter, Index 11 (96% over 28bp)
CCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA40.12311480455524776Illumina PCR Primer Index 11 (100% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT40.12311480455524776TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGACCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT40.12311480455524776Illumina PCR Primer Index 11 (96% over 25bp)
TCTCCGAGCCCAAGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.12311480455524776Illumina PCR Primer Index 11 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA155.655425E-496.492061
ATCTTTT156.030074E-494.984377
TTCCGAT156.030074E-494.984372
GATCTTT156.030074E-494.984376
TCCGATC156.030074E-494.984373
CCGATCT156.030074E-494.984374
CGATCTT156.030074E-494.984375
TCTTTTT250.004579989256.9906238
TAGATCG250.004579989256.9906239
CTTTTTT300.00942245347.4921849
AAGAGCC901.4015495E-723.74609416-17
GATCGGA952.3645771E-722.496310-11
AGATCGG752.4745485E-522.16302110-11
TCGGAAG1003.8801954E-721.37148512-13
GGAAGAG1003.8801954E-721.37148514-15
GAAGAGC1002.2644136E-418.9968766
CGGAAGA1002.2644136E-418.9968764
ATCGGAA1002.2644136E-418.9968762
AGAGCCC1002.2644136E-416.62226518-19
GAGCCCA1952.2059826E-412.3707771
Produced by FastQC (version 0.11.2)