FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s18.351000000d7d50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s18.351000000d7d50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6735010
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT1869242.7754079058531467No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1457342.1638275221566117TruSeq Adapter, Index 12 (95% over 22bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG1142361.696151898809356TruSeq Adapter, Index 12 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT908321.3486542707434732RNA PCR Primer, Index 12 (95% over 24bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT415470.6168810439776631TruSeq Adapter, Index 12 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC322260.4784848129401441No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA297140.4411871697295179RNA PCR Primer, Index 12 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG233790.34712643336832466TruSeq Adapter, Index 12 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT192430.2857159826043317RNA PCR Primer, Index 12 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117380.1742833343974248No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC105150.1561244897928882Illumina PCR Primer Index 5 (96% over 25bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85590.1270822166559515No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80120.11896047667338283No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA79620.11821808727826685No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA76550.1136598163922548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT430650.036.178182
CCGATCT434750.035.520144
CTTCCGA442750.035.425651
TCCGATC444500.034.933373
CGATCTT180500.031.5528985
GATCTTT145050.031.0119846
GATCTAG28900.027.9414146
ATCTTTT157250.027.8795537
CGATCTA89100.027.3487195
CGATCTG184150.026.336095
TCTTTTT188650.022.6852058
CTTTTTT193800.022.1313929
TCTTCCG289550.021.4904255
CGATCTC123900.021.315765
CTAGGAC42650.020.9380443
TAGGACT42700.020.8022844
AGGACTA41000.020.737985
GATCTGG79950.020.3785346
CTCTTCC311300.020.0804794
ATCTAGG45400.019.4783061
Produced by FastQC (version 0.11.2)