FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s17.351000000d7d36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s17.351000000d7d36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA1745.140324963072378Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC661.949778434268833Illumina PCR Primer Index 10 (96% over 25bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA661.949778434268833Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGCT531.565731166912851No Hit
TCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG371.0930576070901035TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG371.0930576070901035Illumina PCR Primer Index 10 (96% over 28bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA320.9453471196454948Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG300.8862629246676514TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG260.7680945347119645Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.7385524372230428No Hit
TCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG240.7090103397341212Illumina PCR Primer Index 10 (96% over 28bp)
TCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG240.7090103397341212Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG220.6499261447562776Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT200.5908419497784343Illumina PCR Primer Index 10 (95% over 21bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA190.5612998522895126Illumina PCR Primer Index 11 (100% over 29bp)
CTTATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGT180.5317577548005908No Hit
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT170.5022156573116691RNA PCR Primer, Index 29 (96% over 27bp)
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA160.4726735598227474Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG160.4726735598227474TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA150.4431314623338257Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAA150.4431314623338257Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT140.413589364844904TruSeq Adapter, Index 10 (96% over 26bp)
CTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA140.413589364844904RNA PCR Primer, Index 10 (96% over 28bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA140.413589364844904Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT140.413589364844904Illumina PCR Primer Index 10 (95% over 23bp)
CTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA130.38404726735598227Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCT130.38404726735598227RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT120.3545051698670606Illumina PCR Primer Index 10 (96% over 27bp)
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA120.3545051698670606Illumina PCR Primer Index 10 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3545051698670606No Hit
ATACACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTT120.3545051698670606TruSeq Adapter, Index 10 (95% over 21bp)
CATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT110.3249630723781388Illumina PCR Primer Index 10 (96% over 26bp)
CTTATACACATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT110.3249630723781388No Hit
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA110.3249630723781388Illumina PCR Primer Index 10 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.29542097488921715No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA100.29542097488921715TruSeq Adapter, Index 11 (96% over 30bp)
CATCTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT100.29542097488921715Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.29542097488921715RNA PCR Primer, Index 29 (96% over 28bp)
CTCCGAGCCCACTAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA100.29542097488921715Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT100.29542097488921715No Hit
TCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG100.29542097488921715TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA90.2658788774002954RNA PCR Primer, Index 10 (96% over 28bp)
CTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.2363367799113737Illumina PCR Primer Index 10 (96% over 28bp)
CTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.2363367799113737Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA80.2363367799113737RNA PCR Primer, Index 29 (96% over 28bp)
TATCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG80.2363367799113737Illumina PCR Primer Index 10 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCTA80.2363367799113737No Hit
CTTATACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT80.2363367799113737No Hit
TCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAA80.2363367799113737Illumina PCR Primer Index 11 (96% over 29bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG70.206794682422452TruSeq Adapter, Index 11 (96% over 30bp)
CATCTCCTAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.206794682422452Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.206794682422452RNA PCR Primer, Index 29 (96% over 28bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTCGC70.206794682422452No Hit
CTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA70.206794682422452TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.206794682422452Illumina PCR Primer Index 10 (96% over 26bp)
GAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAAAAA70.206794682422452RNA PCR Primer, Index 29 (96% over 27bp)
CATCTCCGAGCCCACGATACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.206794682422452TruSeq Adapter, Index 10 (96% over 26bp)
CATCTCCGAGACCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT70.206794682422452TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA70.206794682422452Illumina PCR Primer Index 10 (96% over 28bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA70.206794682422452Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGT60.1772525849335303No Hit
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC60.1772525849335303RNA PCR Primer, Index 11 (96% over 27bp)
CACATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG60.1772525849335303TruSeq Adapter, Index 10 (95% over 24bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA60.1772525849335303Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA60.1772525849335303Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT60.1772525849335303RNA PCR Primer, Index 12 (95% over 24bp)
CATCTCCGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT60.1772525849335303TruSeq Adapter, Index 10 (96% over 26bp)
ACACATCTACGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.14771048744460857Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCAGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.14771048744460857Illumina PCR Primer Index 10 (96% over 27bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14771048744460857No Hit
CATCTCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT50.14771048744460857TruSeq Adapter, Index 11 (96% over 29bp)
TCTCCGAGCCAACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTG50.14771048744460857RNA PCR Primer, Index 29 (96% over 27bp)
CTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG50.14771048744460857TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.14771048744460857RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACTAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG50.14771048744460857Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA50.14771048744460857RNA PCR Primer, Index 11 (96% over 30bp)
ATACACATCTCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT50.14771048744460857Illumina PCR Primer Index 10 (95% over 23bp)
CATCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.14771048744460857Illumina PCR Primer Index 10 (96% over 27bp)
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGGAGCT50.14771048744460857No Hit
CATCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT50.14771048744460857RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT50.14771048744460857No Hit
TCTCCGAGCCCACGGGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.11816838995568685TruSeq Adapter, Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGC40.11816838995568685Illumina PCR Primer Index 11 (96% over 26bp)
TCTCCGAGCCCACGATACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.11816838995568685Illumina PCR Primer Index 11 (96% over 30bp)
TCCGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTGAA40.11816838995568685RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTACGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT40.11816838995568685Illumina PCR Primer Index 11 (100% over 24bp)
CTCCGAGCCAACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGA40.11816838995568685RNA PCR Primer, Index 29 (96% over 27bp)
TCTCCGAGCCCACTAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG40.11816838995568685TruSeq Adapter, Index 11 (96% over 30bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA40.11816838995568685RNA PCR Primer, Index 11 (96% over 30bp)
GGCTCGGAGATGTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGA40.11816838995568685No Hit
TCTCGGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.11816838995568685Illumina PCR Primer Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT40.11816838995568685No Hit
CATCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC40.11816838995568685RNA PCR Primer, Index 29 (96% over 25bp)
GAGCCCACGAGACACTAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA40.11816838995568685RNA PCR Primer, Index 29 (96% over 29bp)
TCCGAGCCCACGAGACACTCGCTAAGCTCGTATGCCGTCTTCTGCTTGAA40.11816838995568685RNA PCR Primer, Index 29 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT40.11816838995568685Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT40.11816838995568685TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCAACGAGACGGAGCTACATCTCGTATGCCGT40.11816838995568685No Hit
TCTCAGAGCCCACGAGACACTCGCTAAGCTCGTATGCCGTCTTCTGCTTG40.11816838995568685RNA PCR Primer, Index 29 (96% over 27bp)
AAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC40.11816838995568685No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGATC201.5014148E-595.000018
AGCAGAT156.0519954E-495.07
CAGATCG352.7312399E-667.857149
TATACAC352.4050323E-454.285713
TTATACA352.4050323E-454.285712
CTTATAC352.4050323E-454.285711
ATACACA500.001396573338.04
AGATCGG409.267254E-635.62510-11
TACACAT550.002231030334.5454565
TCTCCGA803.6174012E-429.6875021
TCCGAGC1152.5699374E-628.9130443
CGGAAGA504.2675267E-528.514-15
ATCGGAA504.2675267E-528.512-13
CACATCT700.00727142827.1428557
ACACATC700.00727142827.1428556
CCGAGCC1254.925574E-626.6000024
CTCCGAG907.214085E-426.3888872
GAAGAGC558.172484E-525.90909216-17
AAGAGCC500.001488869323.7516-17
GATCGGA500.001488869323.7510-11
Produced by FastQC (version 0.11.2)