FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s16.351000000d7d0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s16.351000000d7d0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT21.8867924528301887TruSeq Adapter, Index 11 (95% over 23bp)
ACTTTGTTACTCGTCAGAAAATCGAAATCATCTTCGGTTAAATCCAAAAC10.9433962264150944No Hit
GTTAACGTATTTAGCCACATAGAAACCAACAGCCATATAACTGGTAGCTT10.9433962264150944No Hit
CAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGGTATCAGGGTTAA10.9433962264150944No Hit
AAGTGAGACAGCCTATAAAGCCAGCGCTTGGATCTGTCTCTTATACACAT10.9433962264150944No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAA10.9433962264150944No Hit
TTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATAT10.9433962264150944No Hit
TATGAACACTTCATTTTCTATTATTCATATATATTATATGCTGTCTCTTA10.9433962264150944No Hit
AGTGATTGGACTCTGACTTTTGTCAATCCAGTTTGTGCCCAAAAGAGGTG10.9433962264150944No Hit
ACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAA10.9433962264150944No Hit
CTTCAATACAACACCCTCAATAGTCCATGACTATTGCCCATACGAACTAG10.9433962264150944No Hit
CCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCT10.9433962264150944No Hit
TCTCTAGATATTTATATATATATTCGCGTATATAAATGTTGTACAATCAG10.9433962264150944No Hit
CCCTTGGGGTTGTAGGGCTTCATAAGGTTCTCGGAGAAAGAACGGATGCT10.9433962264150944No Hit
CATTTAAATTTAAACGTGAAACGCAGCAATTCTCGATGGTTATTGTAGTT10.9433962264150944No Hit
ATGCAGCGGCAGCAGCAGCCGGAATGACCACAGAAACCGGAGCTGGACTA10.9433962264150944No Hit
GGTAACCCACTTCGAAGAGACGAACAGAACACCCCAGGACTATGACTTGA10.9433962264150944No Hit
GTGCTCTTCCGATCTTTAGTGGGAGAGAGTTGCTCGATATATGAACGTTG10.9433962264150944No Hit
CATTTAATCACATATAAGAACTCCACCGGTAATACGCTTACATACATAAA10.9433962264150944No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9433962264150944No Hit
CAAACATTGCGGTAAAAGAACGCCGACAAACGACGAACCCGTCGTCGCTT10.9433962264150944No Hit
GCTAGTGTTGTTGTTGTTGCTGTTGCTGCTGCTGCTGCTGCTGCTGTTGT10.9433962264150944No Hit
ACAAACAAGCTGGCAGGCGGAAAAGCAAGCGATCGCAAAGAGCCACATAA10.9433962264150944No Hit
GCTTATGTTGATGCGGCTGAAGTTCAAAAAACTGAAAATGAAGCTGCCAA10.9433962264150944No Hit
CCACTGGGTTCCGTTCGGCCACCGTGCCGGGAAATGCCGCCCAGCGCCCC10.9433962264150944No Hit
GTCCATGACCAGCTCCCCGCCGGCCACACCCACTGGCGTCCAGACGGACT10.9433962264150944No Hit
GGCTTGTTGTTGTTTTCGTTTAAGGGGTATCACTTGCAAGTAACCGAAAA10.9433962264150944No Hit
CTTGATCCTATTGCCACTATCCCTGTACTGCTCACAAACCCCGCGCCCCT10.9433962264150944No Hit
TGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT10.9433962264150944No Hit
ACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCG10.9433962264150944No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT10.9433962264150944RNA PCR Primer, Index 11 (96% over 25bp)
GCCTAAAAGTTGTGATTTTGCTCAAGAATGTAAATAAAAAAAAGGATAAC10.9433962264150944No Hit
CAGATACTCGCTCTCTCACAGATACACACATAGAGTAAGCCTTATGTAGT10.9433962264150944No Hit
GGACACCTTGATGCCGGCGTACAGACAGGCGCGATAGTGGGCATCCACAA10.9433962264150944No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGACGCTG10.9433962264150944No Hit
GCTATGAACGGCTAATGAGTATGCAGCGTTGCTATGATTGCTGTTGCTGA10.9433962264150944No Hit
CCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAG10.9433962264150944No Hit
GCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAA10.9433962264150944No Hit
CCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTACC10.9433962264150944No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT10.9433962264150944No Hit
TCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAG10.9433962264150944No Hit
AGAATGAGCTGAGGATCGTCTCAGAGCAGCTCCTCCTATCCCGCTACACA10.9433962264150944No Hit
GCATTTTTTCACATTCTTGTGAAATAGCTTTTTTTTTTCACATTCTTGTG10.9433962264150944No Hit
ATGCCATCTTGGGCGTCACGGAAGCCTTCAAAAAGGACACCAACCCCAAG10.9433962264150944No Hit
ACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGCGCATAAATT10.9433962264150944No Hit
TATTAACATCGTATTAACGACAACCGTTAACGCAAACAGTTGGTCAAAAG10.9433962264150944No Hit
TCCACTACCATTGTCTGGGTATCTCTGTGTTTTTGTTACCATCACCATCG10.9433962264150944No Hit
ATATAGATCTGCCCAAATGGTGCCACTGTAGCAGCTCGAACTGATCGAAG10.9433962264150944No Hit
AGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTT10.9433962264150944No Hit
CCCAGGACCACCGGGACCAGAGGGACAGAAGGGTGACGCCGGTTTGCCCG10.9433962264150944No Hit
CATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAG10.9433962264150944No Hit
CCAAATCTCTTTCTTCTTGAAAAGAAGAAAAATTGAAGCGCGATTGATCA10.9433962264150944No Hit
ACATAATGGAGCCCGTCATCGACACCCTCCGCATCATCGATATCACCGAT10.9433962264150944No Hit
ATATAGCCGAAAATGAGTGAAAATCATTGTTTTCAGTGTAGTTGGTCCAG10.9433962264150944No Hit
GGCCGGTGAGCGCCTCCGCCACCTCGTGCAGGTGCTGGGCCATCGTCCGG10.9433962264150944No Hit
GCCTCATCTTATTCCGATCTCGCAAAACATTGTTGATGTTCGAGGAGTTC10.9433962264150944No Hit
TAATAACCACCATGATGGCGACCATCCAAAGGATAAACATCATAGGCAGT10.9433962264150944No Hit
CCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATATTGCG10.9433962264150944No Hit
AGCCACTTCTCCTCATCCAACGCGTCAGTTTTTGACAGAATCGTTAGTTG10.9433962264150944No Hit
CCCGTTCTGGCCACGCTTTTTATAGATGGCATAGATGGCCCAGCCGGGTA10.9433962264150944No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA10.9433962264150944RNA PCR Primer, Index 11 (96% over 30bp)
CTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAG10.9433962264150944No Hit
CCAAATGACGACTACTACCACATCTATTGACATTATGGGTCTGCAAGCTG10.9433962264150944No Hit
TCCTAATAAAGTTTTAAAATTCTTTATGAAGAGTTAATAAAACTGTCTCT10.9433962264150944No Hit
TAGACATGGCGCCGCCAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCA10.9433962264150944No Hit
GCATTTAGTAGCGGTAAAGTTAGACCAAACCATGAAACCAACATAAACAT10.9433962264150944No Hit
GGGACGATAATTATTGGCAGAACCAGCCCAGCTATGAAGACATGAAGCAA10.9433962264150944No Hit
TTTTGTGTGTGTGTGTGTGTGTGTGCAGCATCCCCTTCCCGCTATTTTCA10.9433962264150944No Hit
GTGCTGGTCTATCGGCCGGACCGGCGTCTGCAGGTGTGGCGCTTCTTTAG10.9433962264150944No Hit
ATGCCGCGGATTGTTTTCGCTGAATCAGGTTATTAAAGAGATTATTTGTC10.9433962264150944No Hit
GTACATATGTATTTGTTAGCGAGAAAAACAGTTTTCCGCCGCGCCGTAAT10.9433962264150944No Hit
CCTTGTCGGTGCCGGACCAGGAGAAGTACTGGGTGACCAGCTTCTTGGTC10.9433962264150944No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC10.9433962264150944Illumina PCR Primer Index 11 (100% over 26bp)
CTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGG10.9433962264150944No Hit
CATCTTCACTAAGTTAGAAAACAAGACAGTTGGCTAAAAGAAAACAGCAG10.9433962264150944No Hit
GTACAGTACAGTGGAGGACCAACCTGACCCAGTGGCGGTAGAGCTGCACC10.9433962264150944No Hit
CTTGAGCACCATTTGCTGGTTTCACATCTGCCGTTAATTACATGCCACTC10.9433962264150944No Hit
GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAG10.9433962264150944No Hit
ATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCAT10.9433962264150944No Hit
AGAATAGGCACTGTGTCTAGGGATCCTTCTTGGGCCATTAGGTCAGAGCA10.9433962264150944No Hit
GCATTGCAGGGCTACGCGGCGCAGATTATTGGCACTCTCCCATTGTCTCA10.9433962264150944No Hit
CTCTTTCGGGTGGGCTAACCTGCAAAATTCGCGGTTTGTTTTTGGTTCGA10.9433962264150944No Hit
CATGATGGTGGTTGTTATACCGTCAAGGACTGTGTGACTATTGACGTCCT10.9433962264150944No Hit
GCCAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAA10.9433962264150944No Hit
CAGAGTGGTGCAGAGTGCAGTAAGCGGCGGTTTGATGAGTGCCACTGCTC10.9433962264150944No Hit
GGCGGCAAGTCCATCTACGGCAACAAGTTCCCCGATGAGAACTTCGAGCT10.9433962264150944No Hit
CCCCACCAACGAGTACGTGATCTCCACTCGCGTGCGTTGCGGTCGCTCCA10.9433962264150944No Hit
GGCAGATCATCTGCGCCAGCATGTACGAAAAAAGCATCCGGAGATAGCCA10.9433962264150944No Hit
AAGTACTACATTGGCAGGCTCATCTTCTGCTTCAGCTTAGTGCTTTTCCT10.9433962264150944No Hit
TGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCAT10.9433962264150944No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10.9433962264150944No Hit
AGGTTGGATACGCCAATCATTTTTATCGAAGCGCGCATAAATTTGAGCAG10.9433962264150944No Hit
TCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAA10.9433962264150944No Hit
GCGCACGGCCATCAGGCTCTTGGACTTCTTCTCCAGGAACTCCAACTTCT10.9433962264150944No Hit
ATCCTGGCCAGCCACTCTCATGTGGTAGGCACCTTTGTGCCGCAGAACGT10.9433962264150944No Hit
GTCGTTGTCCGAGTGCACCCAACTGCTCCAAAGCAACTTCTTCTGGCTGC10.9433962264150944No Hit
GTACATATGTGCGATAAGAAAAGTTAAAAACAAAAACAGCTGTAAAACTC10.9433962264150944No Hit
CTTCCGATCTGGGTGGGGCCAAAAAGGATTACCATTTCCGGCTGGACGCA10.9433962264150944No Hit
TCTCAGGATTTTGTGGTCAGGCAGTGTAACATAGTGGATAAGAGCTCAGG10.9433962264150944No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG10.9433962264150944No Hit
GTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTC10.9433962264150944No Hit
GTCAGGAGCCAGGAAGAGGCAAGGAAGGGGTCAGAGGGCTGTCTCTTATA10.9433962264150944No Hit
GTGAAGGCGGTGATCTTGGCGATTTTCACCGAATTCGCCTGGCGCTTGTA10.9433962264150944No Hit
GGTATTATGTAGTCTTTTACCAAATGAACTGAGAACTTTGAAGCATTGGA10.9433962264150944No Hit
GGTTTAATAAACACAATCAAATGCATTTAACTAATCACTGATCCTCCTAG10.9433962264150944No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers