FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s14.351000000d7cca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s14.351000000d7cca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1364.402719326642926No Hit
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA391.2625445127873098Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC371.1977986403366785RNA PCR Primer, Index 12 (96% over 26bp)
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA290.9388151505341535Illumina PCR Primer Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC280.9064422143088379Illumina PCR Primer Index 10 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC260.8416963418582066RNA PCR Primer, Index 41 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT220.712204596956944No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.712204596956944No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.6150857882809971No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT140.45322110715441893TruSeq Adapter, Index 11 (95% over 23bp)
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA130.4208481709291033TruSeq Adapter, Index 12 (96% over 29bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA110.356102298478472RNA PCR Primer, Index 17 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG80.2589834898025251No Hit
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA70.22661055357720947TruSeq Adapter, Index 3 (96% over 28bp)
AGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG60.19423761735189382Illumina PCR Primer Index 5 (95% over 24bp)
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA60.19423761735189382TruSeq Adapter, Index 12 (96% over 29bp)
GAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA60.19423761735189382Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCAACGAGACGCGTAGTAATCTCGTATGCCGTCTT60.19423761735189382Illumina PCR Primer Index 12 (95% over 22bp)
TCTCCGAGCCCACGATACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG60.19423761735189382TruSeq Adapter, Index 10 (96% over 29bp)
CTTATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT50.16186468112657817No Hit
ATACACATCTCAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT50.16186468112657817TruSeq Adapter, Index 12 (95% over 22bp)
AAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC50.16186468112657817No Hit
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACGCGTAGT50.16186468112657817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA50.16186468112657817No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT50.16186468112657817RNA PCR Primer, Index 11 (96% over 25bp)
TCTCCGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.12949174490126256Illumina PCR Primer Index 3 (96% over 28bp)
AAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT40.12949174490126256TruSeq Adapter, Index 12 (95% over 24bp)
AAAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT40.12949174490126256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA40.12949174490126256No Hit
TCTCCGAGCCAACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG40.12949174490126256TruSeq Adapter, Index 10 (96% over 29bp)
ATACACATCTCCGAGCACACGAGACGCGTAGTAATCTCGTATGCCGTCTT40.12949174490126256Illumina PCR Primer Index 12 (95% over 22bp)
TCTCAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG40.12949174490126256TruSeq Adapter, Index 3 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG40.12949174490126256No Hit
TCTCCGAGCCCACTAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG40.12949174490126256TruSeq Adapter, Index 12 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC40.12949174490126256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCCC155.9977954E-495.01
TTCCGAT254.4768698E-576.02
TCCGATC254.4768698E-576.03
CTTCCGA254.4768698E-576.01
CCGATCT352.369318E-454.2857174
CGATCTT352.369318E-454.2857175
GATCTTT404.5823466E-447.5000046
ATCTTTT458.1913546E-442.2222257
TCTTTTT458.1913546E-442.2222258
CTTTTTT550.00219845734.5454569
AGAGCCC600.0033695131.6666688
AAGAGCC650.0049875129.230777
GTCTTCT1050.00625750113.57142842-43
Produced by FastQC (version 0.11.2)