FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s13.351000000d7cbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s13.351000000d7cbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6168
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA981.5888456549935148Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGC881.4267185473411155RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT781.264591439688716No Hit
GAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAA661.0700389105058365RNA PCR Primer, Index 29 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT590.956549935149157TruSeq Adapter, Index 11 (95% over 23bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA510.8268482490272374RNA PCR Primer, Index 42 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG460.7457846952010376RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG450.7295719844357976TruSeq Adapter, Index 7 (96% over 28bp)
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA420.6809338521400778RNA PCR Primer, Index 7 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT420.6809338521400778RNA PCR Primer, Index 11 (96% over 25bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG360.5836575875486382TruSeq Adapter, Index 11 (96% over 30bp)
TCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA300.48638132295719844Illumina PCR Primer Index 10 (96% over 29bp)
TCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAA250.40531776913099876TruSeq Adapter, Index 7 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.40531776913099876No Hit
GAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAA220.35667963683527887Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG190.30804150453955903TruSeq Adapter, Index 10 (96% over 29bp)
AGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA170.27561608300907914RNA PCR Primer, Index 29 (96% over 30bp)
AGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA160.25940337224383914TruSeq Adapter, Index 7 (96% over 28bp)
TCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG160.25940337224383914Illumina PCR Primer Index 10 (96% over 29bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT150.24319066147859922TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGATACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG150.24319066147859922TruSeq Adapter, Index 10 (96% over 29bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGA140.2269779507133593RNA PCR Primer, Index 29 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC130.21076523994811933TruSeq Adapter, Index 11 (95% over 21bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACACTGAGC130.21076523994811933No Hit
TCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG130.21076523994811933TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCTAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG120.19455252918287938Illumina Paired End PCR Primer 2 (96% over 29bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA120.19455252918287938Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG110.17833981841763943Illumina PCR Primer Index 7 (96% over 28bp)
CATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTT110.17833981841763943RNA PCR Primer, Index 29 (96% over 29bp)
ACACATCTCAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT110.17833981841763943Illumina PCR Primer Index 10 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT110.17833981841763943RNA PCR Primer, Index 11 (95% over 22bp)
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC110.17833981841763943Illumina Paired End PCR Primer 2 (96% over 26bp)
AGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA110.17833981841763943Illumina Paired End PCR Primer 2 (96% over 29bp)
TCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA110.17833981841763943Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCTAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG100.1621271076523995TruSeq Adapter, Index 7 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTGCAGCT90.14591439688715954No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC90.14591439688715954Illumina PCR Primer Index 10 (96% over 25bp)
ACACATCTCCGAGCCAACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT80.12970168612191957RNA PCR Primer, Index 10 (95% over 24bp)
ACACATCTCCGAGCCAACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT80.12970168612191957Illumina PCR Primer Index 7 (95% over 23bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG80.12970168612191957No Hit
AAAAAAGATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC80.12970168612191957No Hit
TCTCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA80.12970168612191957TruSeq Adapter, Index 7 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG80.12970168612191957TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACTAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG80.12970168612191957RNA PCR Primer, Index 29 (96% over 30bp)
TCTACGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG80.12970168612191957Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCACACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG80.12970168612191957Illumina PCR Primer Index 7 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.12970168612191957No Hit
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA80.12970168612191957TruSeq Adapter, Index 5 (96% over 28bp)
ATACACATCTCAGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT70.11348897535667965TruSeq Adapter, Index 7 (95% over 21bp)
TCTCCGAGCCCACGATACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG70.11348897535667965Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCCACTAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG70.11348897535667965TruSeq Adapter, Index 7 (96% over 28bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA70.11348897535667965RNA PCR Primer, Index 11 (96% over 30bp)
TCTCCGAGCACACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG70.11348897535667965TruSeq Adapter, Index 10 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCA200.001977060271.255
TCTCCGA1750.035.2857131
CTCCGAG1800.034.3055572
TCCGAGC1950.031.6666663
CCGAGCC2001.2732926E-1128.54
GCGCTCA905.4333213E-926.38889120-21
ACTAGCG859.46784E-825.1470616-17
TAGCGCT959.667929E-925.00000218-19
AGACTAG801.623539E-623.74999814-15
GCTCATC801.623539E-623.74999822-23
CGAGCCC1851.7989441E-723.1081095
TACACAT1100.002568071521.590912
TCATCTC904.5186607E-621.11111324-25
ATACACA1150.003324448820.6521761
AGCGCTC901.1123796E-418.47222318-19
CGCTCAT800.001102608417.812520-21
GAGACTA800.001102608417.81259
CGAGACT2203.6379788E-1217.27272812-13
GCCCACG3055.6521458E-817.1311478
GACTAGC850.001655689316.76470814-15
Produced by FastQC (version 0.11.2)