HG5M3BCX2_l01n01_r3zt2s12.351000000d7c94.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l01n01_r3zt2s12.351000000d7c94.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1757
Sequences flagged as poor quality	0
Sequence length	101
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAA	87	4.951622083096187	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	40	2.2766078542970973	Illumina PCR Primer Index 7 (96% over 27bp)
TCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	35	1.9920318725099602	RNA PCR Primer, Index 41 (96% over 31bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	28	1.593625498007968	Illumina PCR Primer Index 7 (96% over 27bp)
AGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA	22	1.2521343198634036	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	15	0.8537279453614115	RNA PCR Primer, Index 41 (96% over 31bp)
CCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA	12	0.6829823562891292	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	12	0.6829823562891292	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	10	0.5691519635742743	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	9	0.512236767216847	Illumina PCR Primer Index 7 (96% over 26bp)
ATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTT	9	0.512236767216847	RNA PCR Primer, Index 41 (95% over 24bp)
CTTATACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGT	8	0.4553215708594195	RNA PCR Primer, Index 41 (95% over 21bp)
CTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	8	0.4553215708594195	RNA PCR Primer, Index 41 (96% over 31bp)
ATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	8	0.4553215708594195	RNA PCR Primer, Index 41 (95% over 24bp)
CTTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	7	0.398406374501992	RNA PCR Primer, Index 41 (95% over 21bp)
TCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.398406374501992	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	7	0.398406374501992	Illumina PCR Primer Index 3 (96% over 26bp)
CTTATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	7	0.398406374501992	RNA PCR Primer, Index 41 (95% over 21bp)
TCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	7	0.398406374501992	Illumina PCR Primer Index 7 (96% over 27bp)
TCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	6	0.3414911781445646	RNA PCR Primer, Index 41 (96% over 31bp)
ACACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	6	0.3414911781445646	RNA PCR Primer, Index 41 (96% over 26bp)
ATACACATCTACGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	6	0.3414911781445646	RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGAGACTCGACGTCAACTCGTATGCCGTCTTCTGCTTG	6	0.3414911781445646	TruSeq Adapter, Index 7 (96% over 26bp)
TCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	6	0.3414911781445646	Illumina PCR Primer Index 3 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTC	5	0.28457598178713717	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	5	0.28457598178713717	RNA PCR Primer, Index 41 (96% over 26bp)
CATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	5	0.28457598178713717	RNA PCR Primer, Index 41 (96% over 29bp)
GGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTCGACGT	4	0.22766078542970974	No Hit
TCCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.22766078542970974	RNA PCR Primer, Index 41 (96% over 31bp)
CTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.22766078542970974	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.22766078542970974	RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.22766078542970974	RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCCACGATACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.22766078542970974	RNA PCR Primer, Index 41 (96% over 29bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	4	0.22766078542970974	No Hit
TCTCCGAGCCCACGAAACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	4	0.22766078542970974	RNA PCR Primer, Index 41 (96% over 31bp)
CATCTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	4	0.22766078542970974	Illumina PCR Primer Index 7 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGACTCGACGTCATCTCGTATGCCGTCT	4	0.22766078542970974	No Hit
GATCGGAAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC	4	0.22766078542970974	Illumina PCR Primer Index 7 (95% over 24bp)
CTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	4	0.22766078542970974	RNA PCR Primer, Index 41 (96% over 31bp)
TATCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	4	0.22766078542970974	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTG	4	0.22766078542970974	TruSeq Adapter, Index 7 (96% over 26bp)
TCTCCGAGCCCACGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	3	0.1707455890722823	RNA PCR Primer, Index 41 (96% over 31bp)
ACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTG	3	0.1707455890722823	RNA PCR Primer, Index 41 (96% over 27bp)
CTCCGAGCCCACTAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.1707455890722823	Illumina PCR Primer Index 7 (96% over 27bp)
CTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.1707455890722823	RNA PCR Primer, Index 41 (96% over 31bp)
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	3	0.1707455890722823	RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTC	3	0.1707455890722823	Illumina PCR Primer Index 3 (95% over 21bp)
CGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAA	3	0.1707455890722823	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCAAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.1707455890722823	RNA PCR Primer, Index 41 (96% over 31bp)
GTACTATAATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAGATCGTTGC	3	0.1707455890722823	No Hit
CTTATACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTC	3	0.1707455890722823	RNA PCR Primer, Index 41 (95% over 22bp)
CTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTGA	3	0.1707455890722823	RNA PCR Primer, Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTC	3	0.1707455890722823	No Hit
ACACATCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTCTG	3	0.1707455890722823	RNA PCR Primer, Index 41 (96% over 27bp)
GTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAG	3	0.1707455890722823	No Hit
CTCCGAGCCCACGAGACTCGACGTCATATCGTATGCCGTCTTCTGCTTGA	3	0.1707455890722823	RNA PCR Primer, Index 7 (96% over 26bp)
GTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGTCCTCCC	3	0.1707455890722823	No Hit
ATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGGCATATCACGTGT	3	0.1707455890722823	No Hit
CTCCGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	3	0.1707455890722823	RNA PCR Primer, Index 41 (96% over 31bp)
TCTCCGAGCCCACAAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	3	0.1707455890722823	Illumina PCR Primer Index 7 (96% over 27bp)
TCTCCGAGCCCACGAACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	3	0.1707455890722823	Illumina PCR Primer Index 3 (96% over 27bp)
CACATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG	2	0.11383039271485487	Illumina PCR Primer Index 3 (95% over 23bp)
TCTCCTAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCTGCTTG	2	0.11383039271485487	TruSeq Adapter, Index 7 (96% over 26bp)
ACACATCTCCGAGGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.11383039271485487	Illumina PCR Primer Index 7 (95% over 22bp)
GTATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGGCATATCTCGT	2	0.11383039271485487	No Hit
TCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 27bp)
CCCGTAACGAGCGCAACCCTTATTGTTAGTTGCCAGCATTGAGTTGGGCA	2	0.11383039271485487	No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGC	2	0.11383039271485487	No Hit
AAGTAACTGTGCGCGTCTTGACGGTACCTAATCAGAAAGCCACGGCTAAC	2	0.11383039271485487	No Hit
ACACATCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.11383039271485487	RNA PCR Primer, Index 41 (96% over 26bp)
GTACAACGAGTGGCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	2	0.11383039271485487	No Hit
TCTCCGAGCCTACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.11383039271485487	RNA PCR Primer, Index 41 (96% over 31bp)
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAG	2	0.11383039271485487	No Hit
CTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA	2	0.11383039271485487	RNA PCR Primer, Index 41 (96% over 31bp)
CTTATACACATCTCCGAGACCACGAGACTCGACGTCATCTCGTATGCCGT	2	0.11383039271485487	RNA PCR Primer, Index 41 (95% over 21bp)
GTCTTGCTAGAGTGCCCAACTCAATGCTGGCAACTAACAATAAGGGTTGC	2	0.11383039271485487	No Hit
CGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATG	2	0.11383039271485487	No Hit
CATATCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 25bp)
TCTCCGAGCCCCCGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 27bp)
GGGCATGACAACTGGTGCACCAGCGGTGCGTCCATCCCGGTCCTCTCGTA	2	0.11383039271485487	No Hit
CTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA	2	0.11383039271485487	No Hit
CAAGTAGTCAGAGCCCGTTTATGGGTGATGGCGTACCTTTTGTAGAATGG	2	0.11383039271485487	No Hit
TCTCCGAGCCCGCGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 27bp)
GGGCATGATGATTTGACGTCATCCCCGCCTTCCTCCGGTTTATCACCGGC	2	0.11383039271485487	No Hit
TCTCCAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 27bp)
CATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.11383039271485487	Illumina PCR Primer Index 3 (96% over 26bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG	2	0.11383039271485487	TruSeq Adapter, Index 11 (96% over 30bp)
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT	2	0.11383039271485487	No Hit
CATCTCCGAGCCCACGGGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.11383039271485487	RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCCACGAGGCTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 25bp)
ATACACATATCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.11383039271485487	No Hit
CACATCTCCAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC	2	0.11383039271485487	Illumina PCR Primer Index 7 (95% over 24bp)
GGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGC	2	0.11383039271485487	No Hit
GGGCTCGGAGATGTGTATAAGAGACAGCTCCGAGCCCACGAGACTCGACG	2	0.11383039271485487	No Hit
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG	2	0.11383039271485487	No Hit
CTAGGGAGTATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGACAT	2	0.11383039271485487	No Hit
CTTATACACATCTCCGAGCCCACGAGCTCGACGTCATCTCGTATGCCGTC	2	0.11383039271485487	RNA PCR Primer, Index 41 (95% over 22bp)
GTCCCTATCCGTCGCGGGCGTTGGAGATTTGAAGAGAGCTATCCTTAGTA	2	0.11383039271485487	No Hit
CTGCTAAACCGTAAGGTGATGTATAGGGGCTGACGCCTGCCCGGTGCTGG	2	0.11383039271485487	No Hit
TTATACACATCTCAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	2	0.11383039271485487	No Hit
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT	2	0.11383039271485487	No Hit
GTATTCACCGTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCAT	2	0.11383039271485487	No Hit
TCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTG	2	0.11383039271485487	TruSeq Adapter, Index 7 (96% over 26bp)
GTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATGATTTG	2	0.11383039271485487	No Hit
GCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	2	0.11383039271485487	RNA PCR Primer, Index 41 (96% over 31bp)
CCCTTAAGCTTAGTTGCCATCATTCAGTTGGGCACTCTAAGTTGACTGCC	2	0.11383039271485487	No Hit
ATCTCCGAGCCAACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.11383039271485487	Illumina PCR Primer Index 3 (96% over 26bp)
TCTCCGAGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.11383039271485487	Illumina PCR Primer Index 3 (96% over 27bp)
ATACACATCTCCGAGCACACGAGACTCGACGTCATCTCGTATGCCGTCTT	2	0.11383039271485487	RNA PCR Primer, Index 41 (95% over 24bp)
GGTAGGAGCCAAGGAACTCTGGACGCTAGTCTAGAGGGAGGCATTGGTGG	2	0.11383039271485487	No Hit
ACACATCTACGAGCCCACGAGACTCGACGTCACCTCGTATGCCGTCTTCT	2	0.11383039271485487	RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCATGAGACTCGACGTCATCTCGTATGCCGT	2	0.11383039271485487	RNA PCR Primer, Index 41 (95% over 21bp)
ATACACATCTCCGAGCCCACGACTCGACGTCATCTCGTATGCCGTCTTCT	2	0.11383039271485487	Illumina PCR Primer Index 7 (95% over 22bp)
CATCTCCTAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 25bp)
TCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.11383039271485487	Illumina PCR Primer Index 3 (96% over 27bp)
GGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAG	2	0.11383039271485487	No Hit
GGTGATAGCCCTGTAACCGACCGAGCGGTCGGCCCATGAGGATCCTGAGT	2	0.11383039271485487	No Hit
TCTCCGAGCCCACGAAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT	2	0.11383039271485487	Illumina PCR Primer Index 3 (96% over 26bp)
ATACACATCTCCGAGCCCAGAGACTCGACGTCATCTCGTATGCCGTCTTC	2	0.11383039271485487	RNA PCR Primer, Index 41 (96% over 25bp)
CCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2	0.11383039271485487	RNA PCR Primer, Index 41 (96% over 31bp)
CTCCGAGGCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA	2	0.11383039271485487	Illumina PCR Primer Index 7 (96% over 27bp)
GTCTCAACGAGAGACTCGGTGAAATCATAGTACCTGTGAAGATGCAGGTT	2	0.11383039271485487	No Hit
ACAATGGATGGTACAACGAGTGGCGAGCCCACGAGACTCGACGTCATCTC	2	0.11383039271485487	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGA	65	8.618967E-5	36.53846	1
CCGAGCC	70	1.334479E-4	33.928574	4
CGAGCCC	85	8.875893E-6	33.52941	5
TACACAT	60	0.0029755356	31.666668	2
ATACACA	60	0.0029755356	31.666668	1
TCCGAGC	80	2.9292735E-4	29.687498	3
CTCCGAG	80	2.9292735E-4	29.687498	2
GCCCACG	110	5.1997384E-5	25.909092	8
GAGCCCA	110	5.1997384E-5	25.909092	6
CCCACGA	110	5.1997384E-5	25.909092	9
AGCCCAC	115	7.04542E-5	24.782608	7
CCACGAG	100	0.0040163053	19.0	5
CACGAGA	100	7.891133E-6	19.0	6
CGAGACT	100	7.891133E-6	19.0	8
ACGAGAC	105	0.0055584363	18.095238	7
AGACTCG	105	1.2040859E-5	18.095238	14-15
GAGACTC	105	0.0055584363	18.095238	9
ACTCGAC	120	3.810868E-5	15.833334	16-17
TGCTTGA	125	5.4124575E-5	15.2	44-45
CTTGAAA	125	5.4124575E-5	15.2	46-47

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content