FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s11.351000000d7c7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s11.351000000d7c7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9869290
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT4587734.648490418257038No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT3475913.5219453476389893TruSeq Adapter, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG2858922.8967838618583506TruSeq Adapter, Index 11 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT2032272.059185615175965RNA PCR Primer, Index 11 (96% over 25bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT920950.9331471666148224TruSeq Adapter, Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC750320.7602573234751436TruSeq Adapter, Index 11 (95% over 21bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA735620.7453626350021126RNA PCR Primer, Index 11 (96% over 30bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG512690.5194801247100855TruSeq Adapter, Index 11 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT447640.4535685951066389RNA PCR Primer, Index 11 (95% over 22bp)
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC231040.23409992005503943Illumina PCR Primer Index 11 (100% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201420.20408762940393887No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.12180207492129627No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111710.1131894999538974No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT106600.10801182253231995TruSeq Adapter, Index 11 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99080.10039222679645649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT135400.031.2922255
GATCTTT148500.026.4526586
TTCCGAT353800.021.4003432
CCGATCT350500.021.3443414
TCCGATC361550.020.8890653
ATCTTTT187100.020.816957
CTTCCGA374350.020.4842951
GTATAAG43550.018.1049358
CTTATAC2748250.017.7551731
TTATACA2804250.017.4415172
TATACAC2812250.017.4341243
CGATCTG103800.016.6111435
TAGACGT14650.016.2114774
TACACAT3117600.015.6960875
ATACACA3203750.015.282914
TCTTCCG307250.014.9803575
ACACATC3336200.014.617796
CACATCT3327000.014.6106547
GAGATGT57350.014.5896111
GTGTATA56500.014.5441356
Produced by FastQC (version 0.11.2)