FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s10.351000000d7c51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s10.351000000d7c51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966793
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT808782.0388762408323298No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT596511.5037588298658386TruSeq Adapter, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG505171.2734972558437003TruSeq Adapter, Index 11 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT384270.968717046742797RNA PCR Primer, Index 11 (96% over 25bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT174460.43980111893915314TruSeq Adapter, Index 11 (96% over 28bp)
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA132790.33475404438799805RNA PCR Primer, Index 11 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC128640.3242921927108372TruSeq Adapter, Index 11 (95% over 21bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT114900.2896546404110323No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG97530.24586611905385533TruSeq Adapter, Index 11 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT76240.1921955594859626RNA PCR Primer, Index 11 (95% over 22bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG67550.17028869416679923No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT67490.1701374384799005No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT66360.1672887897099748No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA62960.1587176341190478No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA60350.15213801173895386No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC58880.14843224740993544No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT54910.1384241627934707No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC51880.13078575060508577No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA50400.12705477699491755No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG50350.12692873058916865No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA49690.1252649180332828No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG46470.11714752950305198No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA46220.11651729747430732No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC45100.11369385798553139Illumina PCR Primer Index 11 (100% over 26bp)
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA42910.10817302541372843No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA42810.10792093260223057No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA42740.10774446763418208No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA42450.10701339848083831No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA41280.10406391258631342No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC41140.10371098265021643No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC40370.10176986800168297No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT39900.10058503178764307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA61700.046.929841
CCGATCT64750.044.1628844
TCCGATC67350.042.7401163
TTCCGAT72100.040.0561332
TCTTCCG32250.030.341585
GCTCTTC40000.024.7004013
CTCTTCC41000.024.445524
TATGCTT34550.024.3346965
TAGGACT59450.022.5319064
CTTGTCT36950.021.9816599
GACTACC59650.021.897837
CTAGGAC62450.021.3734473
ATCTAGG62050.021.301981
AGGACTA62550.021.1873955
ATATAAG24450.020.5933822
GCTTGTC41100.020.4555078
TCTAGGA68900.020.406722
ATGCTTG39600.020.3917476
GGACTAC65600.020.2023126
TATATGC43000.019.994513
Produced by FastQC (version 0.11.2)