FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r3zt2s1.351000000d7b38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r3zt2s1.351000000d7b38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15630248
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT11947527.643845446342246No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT7822645.004808624917532Illumina PCR Primer Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG6788884.343424365371554Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT4960293.1735197035901157Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT3047611.9498155115644997Illumina PCR Primer Index 10 (96% over 26bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA1897141.2137619313525927Illumina PCR Primer Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC1775941.1362199755243807No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG1365940.8739080787457755Illumina PCR Primer Index 10 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT1142050.7306665895512343No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC573920.3671854726809197Illumina PCR Primer Index 10 (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440010.2815118480525709No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396800.25386673327256226No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363640.2326514588892No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT302470.19351580346006025Illumina PCR Primer Index 10 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210300.13454680949400163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT830300.042.8831566
ATCTTTT873050.040.4772267
CGATCTT958000.039.8393825
TCTTTTT1086600.032.605278
CTTTTTT1205250.029.3324139
CCGATCT2200900.027.5494354
TTCCGAT2233000.027.480992
TCCGATC2260850.027.0164093
CTTCCGA2311450.026.755751
CTTATAC4971200.025.3497371
TTATACA5170550.024.3950522
TATACAC5237100.024.0569363
GTATAAG71150.022.3638618
GATCTGG180700.021.2921286
TACACAT6003800.020.9563265
GTGTATA76150.020.8338136
GATGTGT76600.020.773433
ATACACA6089700.020.6505814
GAGATGT78600.020.5038241
GTGCTCT1686900.020.1048811
Produced by FastQC (version 0.11.2)