FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r2zt2wts86.351000000d502d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r2zt2wts86.351000000d502d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT128.16326530612245RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT106.802721088435375No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG42.7210884353741496RNA PCR Primer, Index 29 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT32.0408163265306123RNA PCR Primer, Index 29 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT32.0408163265306123RNA PCR Primer, Index 29 (96% over 25bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTG21.3605442176870748Illumina PCR Primer Index 10 (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6802721088435374No Hit
GTGACGAAGCGTGAGTGACGGTAATGGGTAAAGAAGCACCGGCTAACTAC10.6802721088435374No Hit
TGCTTGTTGTGACCATATATTGTAGTACACAGGACACTAGTTTCAAATTT10.6802721088435374No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCGCTAACCTCGTATGCCGT10.6802721088435374No Hit
GTGAGGCTTCCCAAGGGCAGGTTCAGGGCCGAGGTGGAAAGCGCCTGCAC10.6802721088435374No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTGATCTCGTATGCCGT10.6802721088435374No Hit
CCTTACAGGAGACCCCAAGTGTACAATCTGCCATGATTCAGTGCTAAACC10.6802721088435374No Hit
CATCTCCGAGCCCAAGAGACTACGCTGCATATCGTATGCCGTCTTCTGCT10.6802721088435374Illumina PCR Primer Index 3 (95% over 24bp)
CGTGGGTGCAGGAAGAGGCGGAGTGGCGAACGGGTGAGTAACACGTGAGG10.6802721088435374No Hit
GCCCAGATATATGCCTCACAAGTTGTTTGGCTCTGCTCACCGTTCCTTCG10.6802721088435374No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCCTGTCTCTTATACACA10.6802721088435374No Hit
CAATAGGTATTTGCATGGTTTTATTAAGGTCATTTTGATTTTGTTCATCA10.6802721088435374No Hit
ATACACATCTCCGAGCCCACGAGACTGCCCCTCATCTCGTATGCCGTCTT10.6802721088435374TruSeq Adapter, Index 18 (95% over 21bp)
TCACTTGGTTTCGGGTCTAATCCAACTAACTTAACGCCCTATTCAGACTC10.6802721088435374No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAAGCTCGTATGCCGTCT10.6802721088435374No Hit
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT10.6802721088435374No Hit
GGACAATCCACAGCTTCGGTAATATGTTTAGCCCCGGTACATTTTCGGCG10.6802721088435374No Hit
GATCTGTCCCGGGTAACCTGCATCTTCACAGGTACTATGATTTCACCTGT10.6802721088435374No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAGCAGACCCCCCTGGT10.6802721088435374No Hit
TCGAAGCCCCAGTAAACGGCGGCCGTAACAATAACGGTCCTAAGGTAGCG10.6802721088435374No Hit
TCTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGTCTTCTGCTTG10.6802721088435374Illumina PCR Primer Index 12 (96% over 26bp)
ACCTCGATGTTGGAACAGGACATCCCGATGGTGCAGCCGCTATTAAAGGT10.6802721088435374No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAAGCTCGTATGCCGTC10.6802721088435374No Hit
ATCTGGCGTGGGTGGCATTCGGAGTTTATCAGGATTCGGTACCCAATAAG10.6802721088435374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.6802721088435374No Hit
TCCGATCTCTTCTGGGGTTCTTTTCACCTTTCCCTCACGGTACTGGTTCA10.6802721088435374No Hit
GAACAATTGGAGGGCAAGTCTTGTGCCAGCAGCCGCGGAAATTCCAGCTC10.6802721088435374No Hit
ATACACATCTCCGAGCCCACGAGACTCCAGGTCATCTCGTATGCCGTCTT10.6802721088435374Illumina PCR Primer Index 7 (95% over 24bp)
CGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTG10.6802721088435374No Hit
GAGCACACGCCTCCCTTTTCAGATCGGAAGAGCATACGTCTCCTTTTCAG10.6802721088435374Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTTTATAACAGCGCTGCCAAGATGGCCTACGCCCGTCTGGGAGGCGTGA10.6802721088435374No Hit
GTTAAGAGGAGTGGTTAGCTTCTGCGAAGCCACGAATCGAAGCCCCAGTA10.6802721088435374No Hit
TTATAGTTACGGCCGCCGTTTACTGGGGGTTTCATTTCAGTGCTTCTGTC10.6802721088435374No Hit
CTTCCGATCTCGTGCGGGGTGTGCTCTTCCTGTCTCTTATACACATCTCC10.6802721088435374No Hit
GCACACGTCTCCCGGCTGAGATCGGAAGAGCACACGTCTCCCGGCTGAGA10.6802721088435374Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTGTGGATTGTCCTTTGGACAATGGTAGGAGAGCGTTCTAAGGGCGTCG10.6802721088435374No Hit
GAGGGCTTGTACGACGGCAGCTTCCAGGACGTGATCTATTTCGAGAAAGC10.6802721088435374No Hit
GATCCAGAGATTTCCGAATGGGGGAACCCAACACGAGTTATGTCGTGTTA10.6802721088435374No Hit
ACTTAAGCAGCAACACCAACCCTAGCGTGACCACCTCAATCGGCCCGCTG10.6802721088435374No Hit
GCCCTTTGTATTGTCCATTGTAGCACGTGTGTAGCCCAAATCATAAAGGG10.6802721088435374No Hit
GAGTAAAGAGATTATAGATGAGTTAAAAAGTATGAATGTTGAAGTGTCAA10.6802721088435374No Hit
CGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGCTGTCTCTTATACACA10.6802721088435374No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6802721088435374No Hit
GCATGTCAGATGGTAGACCCGGAGAACTGAAACATCTTAGTCTGTCTCTT10.6802721088435374No Hit
GAAGAGCACCCCGCGGGAGATCGGAAGAGCACCTGTCTCTTATACACATC10.6802721088435374No Hit
GAATAAGGCCAATGTTGGAGGACTCAGGTTATTCAGAACGACAAACAGAC10.6802721088435374No Hit
CGATAACACGACATAACTCGTGTTGGGTTCCCCCATTCGGAAATCTCTGG10.6802721088435374No Hit
GCCTAAATACTCCTTGGTGACCGATAGTGGATGAGTACCGTGAGGGAATG10.6802721088435374No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAAACTCGTATGCCGGCTTCT10.6802721088435374No Hit
ATGGGGACCACGGCGCTTTTGGCCACCTGGGCACAGAAGGGCACATCGGC10.6802721088435374No Hit
CATCAAGCTGAGCTGTGATGGGGAGAGGAAATTGTTTCCTCGAGTCGTTG10.6802721088435374No Hit
TTCGTAGAGGTCGCTGATCTGGCTGGCGCATTCTCTGCAGCGGTCTGCGA10.6802721088435374No Hit
GTTTAGCAGAGACCTGTGTTTTTGATAAACAGTCGCTTGGGCCTATTCAC10.6802721088435374No Hit
TCCTTACGGTCATGCTTCAACGCCCTTAGAACCTGTCTCTTATACACATC10.6802721088435374No Hit
CATCGACCTCATGATGGTGTCTTCTACGTTGCGTTCGTCCTCCTCGGCCG10.6802721088435374No Hit
CGGTTTAGCAGAGACCTGTGTTTTTGATAAACAGTCGCTTGGGCCTATTC10.6802721088435374No Hit
CCGCCAGCGGCAAGTGGAACGTGGAGGTGGGGCGGATTCGGAAGAGCCTG10.6802721088435374No Hit
GGCTATTCTGACTAATGATGATACTCTACATTCAACACCCCACTCTCTTA10.6802721088435374No Hit
CTTCCGATCTGGCACGGGGGCGTTGGAAAAATCAACAAACCCAATACAAA10.6802721088435374No Hit
GATCTAAACCGGGTGCTCTTCCGATCTATATAGGGACTGTCTCTTATACA10.6802721088435374No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCGAAACTCGTATGCCGTCTT10.6802721088435374No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATTCCGT10.6802721088435374No Hit
GTGCGCGTCTTGACGGTACCTAATCAGAAAGCCACGGCTAACACGTGCCA10.6802721088435374No Hit
CAGTAAACGGCGGCCGTAACAATAACGGTCCTAAGGTAGCGAAATTCCTT10.6802721088435374No Hit
AACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAA10.6802721088435374No Hit
CTTCCGATCTTCCCCGGGTGTTACAAACTCTCGTGGTGTGACGGGCGGTG10.6802721088435374No Hit
GGTACTATGATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAAATCGTTA10.6802721088435374No Hit
GCACTAAGGGGCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCG10.6802721088435374No Hit
GCTACAAACAGGGTGAGAGACCCTGTCGCCGAAAGTCCAAGGGTTCCTGC10.6802721088435374No Hit
CCGCGTAGAGCCCGTTGATAACTTCGTTCGGGTTGCCGTTCTTCGGCGCG10.6802721088435374No Hit
GTCCACGAACGATACCATGGCTGAAACGGGATCCACACCTTGAATGTTTG10.6802721088435374No Hit
GGTAAGTTCCGACCCGCACGAAAGGCGTAACGATTTGGGCACTGTCTCAA10.6802721088435374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTGGGTGGGGGGGGGGCGGGG10.6802721088435374No Hit
GAATGAAGCATCGCCAGCACAAGGCCATGCGATTCGAGAAGTTATTATGC10.6802721088435374No Hit
CTTGAGGCTGGGAGTTCGAGACCAGCCTAACCAACATGGAGAAACATTGT10.6802721088435374No Hit
GGGTAATCTTGTGAAACTTCGTCGTGCTGGGGATAGAGCATTGCAATTAT10.6802721088435374No Hit
TCCTAAAAGATAACGGAGGCGCTCAAAGGTTCCCTCAGAATGGTTGGAAA10.6802721088435374No Hit
TATCTGTTCCTCCCAACTCTTGAGAAACCTCTATCCCTACTGCTGCTGTA10.6802721088435374No Hit
CCGTAAGGTGATGTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAA10.6802721088435374No Hit
GCCAATGCTTGAAGACACAACTTGAATGAATGCGCCGCGACGGGCGCGAA10.6802721088435374No Hit
GTCCTGGGACAATCCACAGCTTCGGTAATATGTTTAGCCCCGGTACATTT10.6802721088435374No Hit
CAGCTATGTATTCACTGTGCGATACCCCTAAAAGGGTGGGTTTCCCCATT10.6802721088435374No Hit
GTTTCGGGTCTATACCCAGCGACAAAACGCCCTATTAAGACTCGCTTTCG10.6802721088435374No Hit
GGTTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTT10.6802721088435374No Hit
GGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAG10.6802721088435374No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAAATTCTTATGCCGCCTTCT10.6802721088435374No Hit
ATCTCGTTCTCTGGCTGCTTCCTCCAATTCTACTTTTTTTTCTCCTTGGG10.6802721088435374No Hit
GGACGGAAAGACCCCATGGAGCTTTACTGTAGCTTGATATTGAGTCTGTC10.6802721088435374No Hit
ATATTGAACCGCATGGTTCAAATAGTGAAAGACCTGTCTCTTATACACAT10.6802721088435374No Hit
TCACTGCACACCCCTCAACAGAGCTGACTGTGCCTGACACGTGACTAGCT10.6802721088435374No Hit
AAAAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACCTTTCTTTTATACA10.6802721088435374No Hit
GAGCACACGTCCCCAGTCGAGATCGGAAGAGCACCTGTCTCTTATACACA10.6802721088435374No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGCTAACATCGTATGCCGT10.6802721088435374No Hit
CTTATACACATCTCCAAGCCCACGAGACTGCAGCTAACCGCGTATGCCGT10.6802721088435374No Hit
ATATAGGGCTCTTTTGGGTCTTATAATTGGAATGAGTACAATTTAAATCC10.6802721088435374No Hit
CCTTAGGACCGTTATTGATACGGCCGCCGTTTACTGGGGCTTCGATTCGT10.6802721088435374No Hit
GGATAGAGATTCAGAAAAAGCAGGAACCGATCAGAAGGCCGGAATACACT10.6802721088435374No Hit
GGGAGGGACTATCGGCTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTC10.6802721088435374No Hit
GAGAACAACTTTATTGGATTTGCTTGACCTCGCGGTTTCGCTGCCCCTGT10.6802721088435374No Hit
GCGCTGCTGCTGGCCGCCCGACAGGCGCGTGGGCAAGCGGTCGGCCATGC10.6802721088435374No Hit
CTTCCGATCTGCAATGGGGCGGGCAGTTTGACTGGGGCGGTCGCCTCCTA10.6802721088435374No Hit
CAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGTAGATCAGCATGC10.6802721088435374No Hit
GAAGGCCTTCCGCCGCGATCGCCGCAGCGTCTTTGCATGCTCTAGTTCTT10.6802721088435374No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT10.6802721088435374Illumina PCR Primer Index 10 (95% over 21bp)
GGGCCGTGTATGTGCTGCTAGAGGCTTTTCTTGGCAGCATAGGACCTGTC10.6802721088435374No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTATTCTCGTATGCCGTCTTCT10.6802721088435374Illumina PCR Primer Index 12 (95% over 21bp)
CTCCCGAAGTTACGGGGTCATTTTTGCCGAGTTCCTTAACGAGAGTTCGC10.6802721088435374No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCCT10.6802721088435374No Hit
TCCTAGCAGAACTGTTTTTACCAGTCAATGTCTGGTTGTTTCCATTCCGG10.6802721088435374No Hit
GGTAATTTCTAAGACTTTTTTGTTACTTTCAAGACCTTTCTGTGAAAATA10.6802721088435374No Hit
CAGATACACATCTCCGAGCCCACGAGACTGCAGCTAACCTCGTATGCCGT10.6802721088435374No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC10.6802721088435374No Hit
GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA10.6802721088435374No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers