FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n01_r2zt2wts14.351000000d4e4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n01_r2zt2wts14.351000000d4e4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGATTTGGGCTAC116.666666666666664No Hit
GTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTC116.666666666666664No Hit
CTTATACACATCTCAGAGCCCACCGAGACTGCAGATAACCTCGTATGCCG116.666666666666664No Hit
ATACACATCTCCGAGCCCACGAGACAGTGCTCGATCTCGTATGCCGTCTT116.666666666666664Illumina PCR Primer Index 1 (95% over 21bp)
TATACACATCTCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT116.666666666666664RNA PCR Primer, Index 29 (95% over 23bp)
TTTGTGAACGGCGTCCGGACTGACGAGGGAACATACGGGACCATCGTGCA116.666666666666664No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)