FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30896798
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1167000.3777090428593928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG122450.09.5748870
CGGAAGG55300.08.48060870
AGAGCGG193050.07.55985770
GTAGGGG99550.06.67974270
CGCCGTA29550.06.5137558
GAGCGTG72050.06.31475870
CGGAAGC237150.06.095007470
CTCGGTG70050.05.995431470
AGCGCGG51800.05.87809870
GCCGTAT39900.05.2625579
Produced by FastQC (version 0.11.5)