FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_pIXHALO_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_pIXHALO_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACATAAAACCCACAACATAAATATGTGGTAAGATGACATCATACAAAC11.4285714285714286No Hit
CATTTTCTACTCACTGGACCTCTGTACGGAACTCACCATATCACAAAGTT11.4285714285714286No Hit
CTTGCCGGTGCCGGTGTCTTGGGTGGCGGAGGCTGATTGGTCACATTTAC11.4285714285714286No Hit
CAAAAGGGGAATGATGACTTGACGTAATCCTCACCTTGATCCATCGTATC11.4285714285714286No Hit
CTTCACAAACCTCTTAACCCAGAAACAATCTACGTTGACGTTTACATGAA11.4285714285714286No Hit
AAGTTCCCTCATCATTGGAGTTTCAGATGATTTTACCAAATTTATCTTCG11.4285714285714286No Hit
CGAACTGAGGACGGGTTTTTGGAGTTAGCTCACCCTCGCGGGATCGCGAC11.4285714285714286No Hit
GTGTAGTTTTATTTAATGTCTGAATTTGATGGATCCTTCTGAAACCTTTG11.4285714285714286No Hit
GATGAGTTCTTCTTTACTTTTATCACTTAGGAGCCGTGCGAGATGAAAGT11.4285714285714286No Hit
CGTCAAGGACTCTCAGCCGTCCAATGTTGTTATGGGCTTATAACAGTCGC11.4285714285714286No Hit
CAGGGTGGAAGAAGTGTTATAGGGATATATCTCGACTTACAGCGGAACGA11.4285714285714286No Hit
CCCTATCTTCCGCTTTATTAACTCTTCATTTCGCACTCACATTCCTAAGA11.4285714285714286No Hit
GGAAGGTGAGGATGACGTCAAGTCATCATGCGGCTTATGCACTGGGAGAC11.4285714285714286No Hit
TCAGGTCAGCGTGATGTTCACGGGCTGTTTCTTCATCAACAAAATTACAT11.4285714285714286No Hit
TTGATGTCATGTGTATGATTGAGTATACCAACTTAAACCTCAACACGATC11.4285714285714286No Hit
ATTCACTTCCTCAATAACTGGACCTGGCATCTTCTTCTTATTGAACCTCT11.4285714285714286No Hit
CGAAGTGGAAGCAGGTATGCAGCCGTCAGATTGAGACAAAAGTAATCGGT11.4285714285714286No Hit
ATCAATCACCCACCATGACCCTATACACGTATGTTATAGATATGACGTAT11.4285714285714286No Hit
GGACGAATAGAGCAATCTAGGCATGGGACAGTTATATTACAAATTAATAG11.4285714285714286No Hit
ACAAACACATGGTGACGTCTCACATTGTGATCCATGCATGCATGAAAAAT11.4285714285714286No Hit
ATTAAATATTATTAGCTTTTAAGTTTCTATTTCAAGTGAAATCAGTGAGC11.4285714285714286No Hit
GTTACCTTGAGAATATGTTCACGCTGCGGCTAAACGCACCTATTTTTGGC11.4285714285714286No Hit
AAAATAAACATTTATCAGTCGCTTTCATAAATGTCAAGCAATAAAAAAAG11.4285714285714286No Hit
TGACATGGGAAACCACGTTAGCAAAACTTAATCTTCATTCCCACAGTCGT11.4285714285714286No Hit
GTTGTGATGATGAATTGTGAATGGTGATTGTTTGCCTATATATGTATGTA11.4285714285714286No Hit
TATGAAATCAGCAGGGAATACACCGGATTTGTTCTGGTTCAACTCGTTGC11.4285714285714286No Hit
CATATCTTAGTTGCAGAGGACTGAAAATCCTTATGTCACCATTAGTAGAG11.4285714285714286No Hit
AGAAACTTCAGTTTTGACATCACCAAGACATTAATATCCACGCCAGATCC11.4285714285714286No Hit
TTCCATTATATCACAATTAAACTAATAATATTTCAGAAAATTAATTAAGA11.4285714285714286No Hit
CTTCCAATTATAAAAGATAGAACAAATAGTTCTAATCAGATTGATTGTTA11.4285714285714286No Hit
CCAGTATCCTTCATCGAAACAGAGGATTACTTCATCGCCGACATCAGATC11.4285714285714286No Hit
TATTTACTCTGTTTTTATTTCAAGTTTGGTATTATGATGTCACGAGATAT11.4285714285714286No Hit
GTCGAGGGATATCAGGATGCCTTGTATCCTCACATAGACCGCCAGTTTTA11.4285714285714286No Hit
AGTTGCAATGTTTGTGCTTACCTCACTTGACGTGTGAAATTAATTGTTTT11.4285714285714286No Hit
CCTATGCATCGGCACAAGGGCAAATCAAATGTCATTTGGAAGGGAAATGA11.4285714285714286No Hit
TACCCAGGAAGTTATAACCATGTGATATGTTGTCAATATTATTAATGCTG11.4285714285714286No Hit
AATTTCTATTAGTTTATTGAGAGTGGCAGCTTCCAAGAAACTGCAATCGT11.4285714285714286No Hit
GCACCTGTCCTGTAAGATCCATCTCCGGTACCTATAGAACCGATGACGTC11.4285714285714286No Hit
GTGTGGCAAGCAGTCGTGAGTGGTGTCACTGAGGTCTGGTGCTGAATTCC11.4285714285714286No Hit
ATGAGTTTAAGAAGAAGAAGAAGAAAGAGCAGTACAAGGAGCATCATGAT11.4285714285714286No Hit
ACTAACTGACGTCATCACACGAAGCCCTCCATCTTTATCTAACTTCTTAT11.4285714285714286No Hit
TATATGACTCTGAAATGGATAGCCGAGACCAACCTTGGTCTGGTGTCTCA11.4285714285714286No Hit
CAAAGTAAGGTTGTGGCCACGGCGATGTAGTTGTAAATTCTGGACACTAT11.4285714285714286No Hit
ATTACTAACTAAAATTATCGTCGCCAAAACGTATTAATTTCTTACTATTA11.4285714285714286No Hit
ACCATTGCTTCTTACTAGCTGCGTGGAATCTTGGCCTTATGACATTTCTT11.4285714285714286No Hit
CAAATGAAACTAAACTAGCTGAATGAATATTCTCTTGATGACGTTGCAAA11.4285714285714286No Hit
GAAACTTTACCTCAAAGAACAAACATGAATTATATCCTGTAAGTTCAATG11.4285714285714286No Hit
AAGAAGAGATGGGGTTGACCTTAGTAGTAAATAATCAATTATGCAAAAAT11.4285714285714286No Hit
CTGAACAATGCAATCAAAGAAAAGAGACAAGAGTTGGCTGATCATTTGGC11.4285714285714286No Hit
ATATGTTAATTTGTTAGCTTGTTGCCACTTTTTGATCATTCCGAAAGGAC11.4285714285714286No Hit
GACAACTCTGTTGGAAAATGACATCATGCAAATTTTATCATGCAACTACT11.4285714285714286No Hit
AAAAAGTATATAATGAATTATGAGTTCAATACATCCTGTTTAGCAGAAAG11.4285714285714286No Hit
ATAAGAAAACAATTACATCACTTTTACAAAAATATTATCGACTACTTTGA11.4285714285714286No Hit
AATGATTTAAAATTTAGGAAAGAAGAGAAATTATTGTAGCGTGAGAAAAC11.4285714285714286No Hit
GATTGCTCATAGCGACCATAGAATTTTGTATTTTTTGACAATCATATTAG11.4285714285714286No Hit
ACTTTACGAAAGATTTTAGATAATAGACAAGAAAGTTCTCAATCAAACTC11.4285714285714286No Hit
GCATAGTCTCGTGAAAGAGATAACAGCACTCAATTTGAGTGAGCTAGGGG11.4285714285714286No Hit
GATAAAAAGCATCTACTAATGACGTACTTCATGTGGACATGATGACCGAT11.4285714285714286No Hit
AATCAAACAGGTACACATTAATTAATTAAATGTTTATCGAAATTATTTTC11.4285714285714286No Hit
GATGTCCTGAGGGTGTGATCGAGTGGGTTCTGCGCCTTCTCTGCTGTTCG11.4285714285714286No Hit
AGTAGTATGATATGAATTTTACCTCCCAAAAGGATTACATCAGCTCCGTC11.4285714285714286No Hit
TGCGTGGGTACTTGATAATCGCAAACTGCATTAATAATCTCAAGCTCGGA11.4285714285714286No Hit
ATAGATGATGACGGAATCGATTAACTGAATTATATGCATGACGTAGGAAC11.4285714285714286No Hit
AAAAATGTGCACGACGCGGGCCTACCGGAAATCATAAGGAAGATAGAGGG11.4285714285714286No Hit
CTGAGGACGGGTATTTGGAGTTAGCTCACCCTCGCGGGATCGCTAACCTT11.4285714285714286No Hit
TAACAACATTCCACATGACATTTTTTATTTGGGGCGACCCTAGTGAAAAT11.4285714285714286No Hit
ACTTCTCAATTTGCATACGTATACAACAAACTGTTACATCAGCCTCCTCG11.4285714285714286No Hit
AGAGAAGCGAGAGAGAGAGAAGCGTGAAACACGGAAGAGATGAAAATAGT11.4285714285714286No Hit
TTACACTCGTAGTCTCTGAAGGATGAGAACCACCTATGTGGCCTCTACAT11.4285714285714286No Hit
TTTAAGATTTACGTATATTATATATAAAAAAACGTTCATTCAAATGTATT11.4285714285714286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAT50.070.032
TGTGAAT50.070.052
GTTTGGT50.070.024
TCAAGTT50.070.020
GTCACGA50.070.039
ATCTTGT50.070.062
TTGTATT50.070.065
TTGTATC50.070.058
TGGTATT50.070.027
GATATTT50.070.046
TTACTCT50.070.04
TTCAAGT50.070.019
ATGATGT50.070.034
TTATTTC50.070.015
TGTTTTT50.070.010
GTATCTT50.070.060
GTATTTT50.070.067
GATGTCA50.070.036
TTTTTAT50.070.012
TTTTTAA50.070.070
Produced by FastQC (version 0.11.5)