FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_pIXHALO_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_pIXHALO_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5368317
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC4400.017.49992244
CCCGATC25050.013.69255342
GCCTAAG48750.012.85122555
CAACCCG23200.012.67235839
ACCCGAT26250.012.66661141
ACCGCAA57050.012.20864835
AAACCGC63900.011.99536233
CCGATCT28650.011.97202243
CCTTGTT59700.011.95974670
TGGTCGC6850.011.75177143
CCTAAGT50050.011.67826956
AACCGCA64500.011.55819434
CGCAACC44700.011.51012137
AACCCGA27750.011.351340
CCGCAAC55100.011.17972736
AGCCTAA56850.010.95861354
AGGCGTA20250.010.8888453
TCCTTGT66750.010.8014569
TAAACCG73700.010.73275232
AAGCCTA57350.010.5579353
Produced by FastQC (version 0.11.5)