FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_pIXHALO_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_pIXHALO_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5368317
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC4350.018.50566544
CCCGATC25050.012.57479342
GCCTAAG49200.012.4491355
TGGTCGC6750.012.44438843
ACCGCAA57200.011.99306635
ACCCGAT26050.011.9577241
CAACCCG23200.011.76718939
CCTTGTT59650.011.73506870
AAACCGC64250.011.65764433
AACCGCA64350.011.31318634
CCTAAGT50500.011.22767256
AGGCGTA20600.011.04363953
CGCAACC44950.010.97891837
CCGATCT28750.010.95647243
CCGCAAC55150.010.91573636
AAGGCGT21700.010.80640352
AGCCTAA57000.010.68416254
AACCCGA27600.010.65212640
TCCTTGT66700.010.65212569
AGAGCGG7600.010.59205870
Produced by FastQC (version 0.11.5)