FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_pIXHALO_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_pIXHALO_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTAATATCAGATGGCACAAACTGCATCAATGAATCAAAAGTTACTTGT11.9230769230769231No Hit
ACGTATTTGTTGTCGAGAGTCTTTGATTTGTGGAACTGGGATGACGTCAT11.9230769230769231No Hit
ACTCATACAAACTTGGGCTACACCATGGAAGCTTAGAGAAGCTAGAAGAG11.9230769230769231No Hit
ATTTAGGACCACATGTGGTGATCAAAGACTCAATATATATAGATTGATAT11.9230769230769231No Hit
GCTGCCACTACTGATGGATGTCATCGTCTTCATTGCTGGCCGCCTTGTTT11.9230769230769231No Hit
AACTCTCATTAGGCTTTTAAGATCGGGTTGCGGTTTAAGTTCTAATACTC11.9230769230769231No Hit
GGCGTCATGCTTTAGTGTATGATATTAACTCTGCGAATGAAACCTGGGAA11.9230769230769231No Hit
ATGTTTGAGATGCGTATGTATAAGAAACAAAAAAAAGCCAACATGAGTTA11.9230769230769231No Hit
AAGCAATGGGCTAATGTCGGCTGGATCGAAAAAGACCTCTCAGCTATATA11.9230769230769231No Hit
CTTAGTCTTAATTCCAAACAATAACCAAAAGTCATATTCATTTTCATTTT11.9230769230769231No Hit
ATTATAATCTCCAAGGAGATTTGTATCAGTACTACTTTTGATGTCTCATT11.9230769230769231No Hit
TGTTATTCAATCAAAATAAGGTTCGAATTAATGTTTGTCTGATTTGGGGA11.9230769230769231No Hit
TAACGCAGGTGTCCTAAGATGAGCTCAACGAGCACAGAAATCTCGTGTGG11.9230769230769231No Hit
AACTAATATACCTAAATGTGTCGTCAGTGCACGTTTATGGACATTTATTG11.9230769230769231No Hit
TCATTCCCTTTTCCTGCTTCCCCGGCTGAAACAATAGTTTTTATCATTAG11.9230769230769231No Hit
AGGGGAACTTGAACCGGGTGATATAGAGAATATAGATGAGGAGAATGGGG11.9230769230769231No Hit
CGAACACACAAGCCATCGAATTTGAGATTTACTCTGAGGAAACAACTTGA11.9230769230769231No Hit
ACATCATTGCATAAGATATTGTATTCTCTTACCTAGACCTTGCCGTTTTT11.9230769230769231No Hit
ATCTGTGAACCGTGAAATGTTACGTAACGCAGTGGCCTCAAAACAATATT11.9230769230769231No Hit
ATCGTCAAGAAAAGAGTTAAACCCTGATCAGCTGATGTCTTGCACTAAGC11.9230769230769231No Hit
CGTATAACAACATCAAGATCACAATTCTCTGTGGTTTCGTCACAATTCTC11.9230769230769231No Hit
AAGATCCTATCAGAAATAATTAAGTAACTATCTGCTACCAAACTGATTCT11.9230769230769231No Hit
AAAGCCGTCCATGATATCGCCGATATGACTGCTGACATCATAAAAACGTT11.9230769230769231No Hit
AACAGAAAGCTGTTAGAGGCAGGTTACGTATCGAACACGTCATCGGTATG11.9230769230769231No Hit
TTCAATTTCATTCACTACGCATCAGACAACTAGCAACGATGAACCAAAAT11.9230769230769231No Hit
GAAGTCTATTGTCCTTTTTCTGTCCCAAAACAGCTCAAACTGCATAATCT11.9230769230769231No Hit
AAGACATTGAAAAGAGAGGTACACGAGACGATAATGAGGATGAGGAGAAT11.9230769230769231No Hit
AAAGCACTTTTATGCCTACTTAATTCTGGCGTTGAGGGTGCACTTGAAGA11.9230769230769231No Hit
ATTGAAAATTGATTTTTCACGTTCTATGAAACTATAGAAAATTATATTTC11.9230769230769231No Hit
GATTGTAGTTTTGAGTTATATCGAAATCCTTAGAACTTATTAAATTACTA11.9230769230769231No Hit
TTATAAGGCGGAGGAATTTTTCAGAGAGTGGTGTAAGAGATGACGTCATC11.9230769230769231No Hit
AACATCAAATTCGATTTCAGGTGTTTTGGTGATTTCCGACATGCTGATGT11.9230769230769231No Hit
CAATGATCCCACTTAAATCCATTTTTATTTCATTTTTGATTTTTGATATT11.9230769230769231No Hit
ACTTACATGTGGAAACTTTGGGATACCAGAAGCTCATGGATATGAGAAGG11.9230769230769231No Hit
AAACTACTAATTAAAATGATAATCGGAAAATTGTTAAACATAAGTTTTGA11.9230769230769231No Hit
ATCACACAGCAAGCAATAAGACACAGTACTAAACATAAGAATGTGACGTA11.9230769230769231No Hit
AAAATCTTCCACGAAGGTGAGAGATTGGATATAAGTTTTCGGTGACGGTG11.9230769230769231No Hit
TGTAGGATATACAAGAAGAGAGGAGCAAGTAAGCTTCTGAATGAGCAAGC11.9230769230769231No Hit
CATTTTTTGGATAATCTACATTGGATTAAAGAAATTTCGAAGTTTTTGTC11.9230769230769231No Hit
CGACTCAATGATTGACTATAGATTTGTGTGCATTGTGACAGTTGGCGCAG11.9230769230769231No Hit
TAAGGGGTGACCCGAATGGGCTAAAACCTGAACCGGTCGATATCATGTCT11.9230769230769231No Hit
AAAAATGACGCGGTATCGTCATGATAGTGTCATGATTACATGGTTTAAAA11.9230769230769231No Hit
TTCGAGAGAAGGAACTACACCTACGGCCAGGACGGTCTACACTTAGGATT11.9230769230769231No Hit
CTTTGGAAGATTTGGATAAAAATCGCAAAGATAATATATATACAAACAGA11.9230769230769231No Hit
ATATGCTTACATGTACATATATATGCGTGACGTTTGCATGCCTAATTATA11.9230769230769231No Hit
CTGCAGCTAGGTAATTAACAATATCAGCATACCATGGGTAATCCTTCTCG11.9230769230769231No Hit
GTACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGGTT11.9230769230769231No Hit
AATCCAAATGGGGGTGATGCAAGCAGATGAGAGGTGATCAGAAGGCACTA11.9230769230769231No Hit
TTATTACAATTCAATAATCAATACAATATAATAAGAATTCAAATGAAAGT11.9230769230769231No Hit
TTTACATTTATTTAGCGTTTGATCATTGAGAGGATGCCACAATCTTTAGC11.9230769230769231No Hit
ATAATAATTTGTAAGTCAGTTTAGTTTGTTCCTTTATTATCTTTTTGTTA11.9230769230769231No Hit
GTTAAAAAGTATTCTTAACTTTTTGAGTTATAAAATTCGCAATTGAATAT11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAGT50.070.022
CAAGATT50.070.070
AAGTATT50.070.07
TTTTGAG50.070.021
ATCTCTT50.070.049
AAACATC50.070.062
AAATTCG50.070.033
TTCGCAA50.070.036
TTCGTAA50.070.054
AATTCGC50.070.034
TAAAATT50.070.031
CACAAGA50.070.068
TAAAAAG50.070.03
TAAAAAA50.070.058
TATAAAA50.070.029
CTCTTCG50.070.051
TTTTTGA50.070.020
GTATTCT50.070.09
CAATTGA50.070.040
TTGAGTT50.070.023
Produced by FastQC (version 0.11.5)