FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_coldna_daplib_itru-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_coldna_daplib_itru-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295527
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32170.1401421111579171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG6900.017.24637670
TTCGCCG8750.013.670
GTAGGGG3901.549779E-913.46153870
AGCCTGT4701.9463187E-911.91489470
CGCCTGT7700.010.90909169
GCGTCGG4551.897879E-710.7692370
GGAAGAC6855.456968E-1210.72992670
TAGCCTG5251.10158E-810.66666869
GCCTGTG10900.09.95412870
TCGCCTG10250.09.90243968
GCCAGTG3551.897476E-49.85915668
CCTGTGT10400.09.75961670
TCGCCAG3602.1844686E-49.72222170
CGGAAGC13800.09.13043470
CTTCGCC16900.09.11242669
GGGAAAA14950.08.89632170
CAGTGTA5552.863244E-68.82882970
GTCTTCG22400.08.2812570
TCGTGTT26950.07.792207770
TCGCCGG6855.7760644E-67.66423370
Produced by FastQC (version 0.11.5)