FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_coldna_daplib_dapv2-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_coldna_daplib_dapv2-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4174332
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172380.4129522999128963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCA5200.022.21192
CGGGCTG4900.022.1426913
AGCGCAG4800.021.1459273
GCGCAGG4950.020.5053884
GTCCGGG6050.017.3552410
GCAGGTC6200.016.371046
CCGGGCT7650.015.55543912
TCCGGGC6650.015.26304311
GGTCCGG6500.015.0768099
CGCAGGT7450.015.0336245
TGTGCCG38050.014.99349670
GCGTCGG44450.011.49611470
TGCCTCG24850.011.40850270
TGTGGCG28600.011.38116970
GGGCGCT3802.8016602E-611.05268169
GGCTGAT9500.011.05254815
CGATGTG18600.010.72585570
GGGCTGA10200.010.63717514
AGAGTGG46850.010.53366570
CGCTATG18950.010.52775170
Produced by FastQC (version 0.11.5)