FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_coldna_daplib_dapv1-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_coldna_daplib_dapv1-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566190
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85340.3325552667573328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG7750.023.03214870
CGGTGGG7350.020.47609370
GTAGGGG17250.014.40572970
GCGTCGG16950.013.42176670
TCGGTGG21250.013.17640969
CGCCGTA2952.0211028E-613.05078647
CTCGGTG20050.012.74308270
CTCGGGG5255.456968E-1212.66660670
AGCGCGG2801.69526E-512.4999470
TCGGGGG6950.012.08627370
GCCGTAT2902.4297737E-512.06890948
GGTCGCC8400.011.66661270
TGGTCGC9950.011.60798570
AGAGCGG24150.011.59414970
GAGTGTT27650.011.51893370
AAAGAGG24550.011.26267670
GTCGGTG5703.0377123E-1011.0525870
GGGTCTC2250.006704650410.8888385
ATCGGGG2953.8038805E-410.67791669
CTAGGTG4351.2384444E-610.45972170
Produced by FastQC (version 0.11.5)