FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_coldna_daplib_dapv1-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_coldna_daplib_dapv1-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566190
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85340.3325552667573328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG7800.023.78193770
CGGTGGG7350.020.47609370
GTAGGGG17450.014.84233770
GCGTCGG16850.013.50142170
CTCGGTG20200.012.994987570
TCGGTGG21300.012.98115969
CTCGGGG5153.6379788E-1212.9125670
AGCGCGG2801.69526E-512.4999470
TCGGGGG6800.012.35288270
GGTCGCC8400.011.66661270
CGCCGTA3003.437275E-511.66661247
TGGTCGC9900.011.66661170
AGAGCGG24100.011.61820270
GAGTGTT27800.011.4567870
AAAGAGG24750.011.17166470
GCCGTAT2852.764769E-411.0525848
GGGTCTC2250.006704650410.8888385
ATCGGGG2903.2477916E-410.86201869
CTAGGTG4207.8847916E-710.83328270
GTCGGTG5653.075911E-910.53092570
Produced by FastQC (version 0.11.5)