FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_coldna_daplib_dapv1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_coldna_daplib_dapv1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACCCACAAACTATAAGAATCTCTCGTCATTTCCAGGGTGTGTCTTTC13.8461538461538463No Hit
TCATTTCATCCGAGAACACCCATCTTATTTTCAACAATGTATTTGTCATT13.8461538461538463No Hit
AAGCTTTCATGGTGAAGCTAAAGTCCATATGAGTCTGTGGCTTTGAGTCT13.8461538461538463No Hit
AAGCTTCAAATCGGGACTTATCTTATTCTCACCATCTAAACGAACAAGCT13.8461538461538463No Hit
GTCCTTACTTACCAGACCGGGCTACTTGAATAGGCAGAAAGAAAAAATGT13.8461538461538463No Hit
ATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGTGGG13.8461538461538463No Hit
GAATCAACATTTGACGGATTTTCTCTTCCATCCTTGTTATCCAAAGTCGT13.8461538461538463No Hit
TATATGGCGATGAATTATCTACTAGCTAGATTTTTTTACGAGTAACACTT13.8461538461538463No Hit
GGTCGATGAAGTCTCGGACCTCGTCGACTATTTCAACCCCTGGTCGACAA13.8461538461538463No Hit
CCCTGTCTACTATCCAGCGAACCCACAGCCAAGGGAACGGAGATCGGAAG13.8461538461538463No Hit
ATGATTGAGTGTAAGAACTTAACCCGCAACCCGATCTTAAAAGCCTAAGT13.8461538461538463No Hit
CTTAGTCTTACAAGATCGGGTTGAGGTTTAAGTTGTTATACTCAATGAAA13.8461538461538463No Hit
AAGCTTTCATGGTGTAGCCAAAGTCCATATGAGTCTTTGGCTTTGTGTCT13.8461538461538463No Hit
CACCATGGTAGGCCACTCTCCTACCCTCGAAAGTTGATAGGGCAGAAAGT13.8461538461538463No Hit
GATAACGACCGGGTCAAAATTTGGATTTCAAATAAAGATGCTAATTAGTG13.8461538461538463No Hit
CATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGGTGAGTGTTTTGGAGTCG13.8461538461538463No Hit
CTTTTATGTTAATTGGTTTTGACATAAAAGAGATCGGAAGAGCGTCGTGT13.8461538461538463No Hit
CAAGTAAAGACTACTTATGCTTTTAAGATCGGGTTGCGGTGTAAGATGAT13.8461538461538463No Hit
AAGGTATGTTCGTTATACCTTTCATGAGTCGTTTAGGAATCTCCAATTAT13.8461538461538463No Hit
GTTGCGGTTTAAGTTGTTATACTCAATCATACACATGACATCAATTCATA13.8461538461538463No Hit
TGCAGTTTTAGTTATTATACTCAATAATACACATGACATCAAGTCATATT13.8461538461538463No Hit
AAAGGCGTAAGAATTGTATCCTTGTTAGCAGAGATAAATCCAAAGCCTCA13.8461538461538463No Hit
ACTTCACCGCATCATTCAATCTGTCGGCTCGCCTGGCGGAGTGTAAAAAG13.8461538461538463No Hit
TCTACCAAGCTTAATCGGAATAAGACCTCACAACCACTAGATCGGAAGCG13.8461538461538463No Hit
ATAACAACTTAAACCGCAACACGATCTTGTAAGCCTAAAGATAGGAAGAG13.8461538461538463No Hit
GTGAGGGGGTAGGGGTAGCTATATTTCTGGGAGCGAACTCCATGCTAATA13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)