FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_c24dna_daplib_itru-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_c24dna_daplib_itru-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772154
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66260.37389527095275016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2155.5973942E-817.90689759
GCCGTAT2602.7672286E-816.15377460
AGAGCGG6700.013.5820370
GCGTCGG4803.3736796E-810.93745170
CCGTATC3309.0667076E-510.60601361
TCGCCGT4351.2376222E-610.45972458
TCGGTGG4055.9992417E-610.37032451
CGGTGGT4401.6069398E-59.54541252
GGTGGTC4653.086013E-59.03221753
CGTATCA3600.00235578378.74996162
GTCGCCG7251.9250365E-88.68961757
GTGGTCG5704.102485E-68.59645354
TGGTCGC6603.7493373E-78.48481155
ATCTCGG4450.00180293497.86513448
GGTCGCC7552.8591912E-67.41718656
GTGTTAA21500.07.32554970
CGGAAGC11951.10958354E-107.322143670
GCGCGTC6056.536556E-46.942117769
CGTGGAG14151.4551915E-116.925764670
AGTGTTA24150.06.66663769
Produced by FastQC (version 0.11.5)