FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_c24dna_daplib_dapv2-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_c24dna_daplib_dapv2-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435629
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157190.6453774363829631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2852.6739144E-1017.19271760
GCGTCGG13300.016.31587270
ATAGGGG6450.015.19387470
CGCCGTA2604.867761E-714.80746259
CCGTATC3101.5037585E-814.67719161
TCGGTGG5450.014.1285151
CCCGCCC1550.00737829313.54817715
CTCGGTG4103.268724E-912.80494250
AGAGCGG19050.012.49350170
GAGCGTG6000.012.2500670
CGTATCA4409.340511E-911.93163362
GGTAAGG4351.0199801E-711.26442470
GTAGGGG14350.011.21956870
GGATAGG24550.011.12021870
AAAGAGG16450.010.85111870
GGAAGAC7850.010.70069170
GATAGGG25700.010.07787169
TAGGGTG17950.09.55436570
CGGAAGC31650.09.51031770
TCGCCGT4602.7186197E-59.13029358
Produced by FastQC (version 0.11.5)