FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_c24dna_daplib_dapv1-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_c24dna_daplib_dapv1-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930405
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76860.39815479135207377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2451.8189894E-1221.42797747
GCCGTAT2950.020.16893248
TCTCGGG2806.8817826E-814.99958470
GCCGGAT1550.007378158613.54801248
CGGAAGC11400.012.8943870
CCGTATA1950.002301732312.56375349
GGTCGCC7300.011.5065344
AGAGCGG6800.011.32321570
TCGCCGT4954.3910404E-911.31281746
CCGTATC3551.59251E-510.84476949
TGGTCGC6754.9112714E-1110.37008343
GGTGGTC6856.548362E-1110.21895941
GTCGCCG7201.6370905E-1110.2080545
GTAGGGG6151.236549E-89.67452970
ATCTCGG12350.09.6353669
ATCGGTG3000.00522677379.33307570
CGTATCA4905.6674544E-69.28545750
GCGTCGG5754.708363E-79.13018170
GAGGGTA5058.307425E-69.00965168
AGCGGCG3150.00780424768.88864267
Produced by FastQC (version 0.11.5)