FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_c24dna_daplib_dapv1-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_c24dna_daplib_dapv1-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930405
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76860.39815479135207377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2402.3646862E-1120.416147
GCCGTAT2900.019.3098148
TCTCGGG2858.5485226E-814.73643470
GCCGGAT1600.00910066513.12463748
CGGAAGC11550.012.72691970
AGAGCGG6850.011.24056370
GGTCGCC7300.011.02709144
CCGTATC3551.59251E-510.84476949
TCGCCGT5006.14873E-810.49970946
TGGTCGC6704.7475623E-109.92509743
GTCGCCG7151.4915713E-109.78993845
GGTGGTC6806.220944E-109.77939341
GTAGGGG6151.236549E-89.67452970
ATCTCGG12500.09.51973669
ATCGGTG3000.00522677379.33307570
CGTATCA4905.6674544E-69.28545750
GAGGGTA4956.447084E-69.19166468
GGGAAAA11500.08.82584270
AGCGGCG3200.0088787548.74975867
GATCTCG11650.08.71220568
Produced by FastQC (version 0.11.5)