FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_abdna_ablib_itru-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_abdna_ablib_itru-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771713
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54680.19727872258058465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2400.026.25019656
CGCCGTA2152.6357156E-919.5350359
GCCGTAT3250.019.38476260
TGGTCGC2801.0913936E-1118.7501455
AGCGCGG1503.1614065E-416.33316270
GTCGCCG3552.1827873E-1115.77476757
AGTACGG9600.014.58317970
GCGTCGG7100.014.29562470
GGTGGCC1600.00909985713.12509956
CGCCCTA1600.00909985713.12509959
GTGGTCG6150.013.08952854
CCGTATC4103.270543E-912.80474361
CGGTGGT5255.456968E-1212.666532552
AGAGCGG11950.012.59400970
CGTATCA6250.012.31986962
ATCTCGG10750.012.04638470
GTCGCCC2356.8513217E-411.91498357
CTCGGTG4802.7102942E-911.66654470
CAGTACC7500.010.73321970
TCGGTGG5251.1021257E-810.666553551
Produced by FastQC (version 0.11.5)