FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_abdna_ablib_dapv1-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_abdna_ablib_dapv1-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522322
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42390.16805943095290768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG6100.021.80326770
CGGTGGG8700.020.51723170
TCTCGGG10850.018.38708770
GTCGCCC3650.018.21916870
GCCGTAT7600.017.4999948
CCGTATC5700.016.57893849
CGCCGTA7400.016.08107247
CGTATCA6700.015.67163350
GTCGCCG18150.014.84847669
GGCCGTA2151.977305E-514.651154570
CGCCGTT3604.674803E-1014.58332570
GCGTCGG5300.014.52829570
TGGCCGG3555.989932E-913.80280970
AGGTGGG4506.91216E-1113.22221470
GGTCGCC22050.013.01586469
GGTGGTG20050.012.91769970
GGGCGCC2203.9320454E-412.72726568
TGGTCGC26250.012.39999270
GGCGCCG2601.1774502E-412.11537769
GTGGTCG36000.012.05554870
Produced by FastQC (version 0.11.5)