FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_abdna_ablib_dapv1-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_abdna_ablib_dapv1-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522322
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42390.16805943095290768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG6050.021.40494570
CGGTGGG8650.020.63582670
TCTCGGG10600.019.15093270
GTCGCCC3750.018.66665670
GCCGTAT7700.017.27271848
CGCCGTA7400.016.08107247
CCGTATC5800.015.68964749
CGTATCA6800.014.92646250
GTCGCCG18100.014.69612469
GCGTCGG5300.014.52829570
CGCCGTT3504.9058144E-913.99999170
TGGCCGG3555.989932E-913.80280970
GGCCGTA2303.7336613E-513.69564470
GGTCGCC22150.013.27312969
GGGCGCC2203.9320454E-412.72726568
GGTGGTG19900.012.66330970
TGGTCGC26350.012.35293470
GGCGCCG2601.1774502E-412.11537769
GTGGTCG36000.011.95832570
AGGTGGG4451.1041266E-811.79774670
Produced by FastQC (version 0.11.5)