FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_abdna_ablib_dapv1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_abdna_ablib_dapv1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATATTACTAGATGTCATGTGTATGAGTGACTATGACAACTAGAACAAT17.142857142857142No Hit
GAACACAATCAAATCACCGTAATTTCAGACAGATCATGGGAGATCGGAAG17.142857142857142No Hit
AGCATCCCCTTTGAGCTTAAAAATTACTTTAGATCGGAAGAGCGTAGAGT17.142857142857142No Hit
GTGATGGATTTGGTGAGAGTGCTCTGATGCAGTAAAGCTTGAGGATGTAT17.142857142857142No Hit
ATATCTATATTAGGTACATAGATATAGATCGGAAGAGCGTCGTTTAGGGA17.142857142857142Illumina Single End PCR Primer 1 (96% over 25bp)
ACCGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAGCCACAAAGC17.142857142857142No Hit
ATAAATGAACCACAGAGGCAGTGGCATTGTCCAGCTTGTCAGAACGGACC17.142857142857142No Hit
AAAAGCTGAACTATGCCTGAGCTTGGAAAAGCCAGAGGAAACTCTGGTGG17.142857142857142No Hit
CACTAATTGAGAAGTCAAGTGATATTGAGATCGGAAGAGAGTCGAGTAAG17.142857142857142No Hit
AGAGATAACAGGAGAAATTGATTGCTTAAGGACTAGAGCAACTTGTTGCA17.142857142857142No Hit
CCCATTAACTATGCCACAGAGCTATCTTCCTCTCTACTTTTTCGCCACCA17.142857142857142No Hit
AAGGTATCAGTGTTAAGGACGCGAGCTTGCTTAACGCCGAAATCATTGCA17.142857142857142No Hit
ATCTTCTTCTTCTCCGTCTCCATCGCCACCCTCCGACAAGAAGAAGACCA17.142857142857142No Hit
ATCTTGATTAAGATTGACCAAAATGATCTCTTCTTAAATCATCACGTGAT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)