FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WRKY40_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WRKY40_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216646
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG10450.013.39725470
AGCGCGG2408.1760687E-411.66677570
CGGAAGC22450.010.91324170
AGAGCGG16750.010.02994470
CTCGGGG2900.0039518429.65526370
GAGCGTG5401.9764248E-69.07415970
GTAGGGG7452.751931E-77.98665270
AGTGTAG22150.07.742735470
CCCTGCC5200.0010091697.403800565
TCGTGTT30750.07.28462170
CCTGCCC5450.00166078327.064176666
GTAGGGT11201.2332748E-86.87506470
TGGAGGG24800.06.633126370
GTCGTGG47500.06.55795670
GTAGGGA41400.06.425180467
GTGTAGA20350.06.363695669
GAGTGTA26850.06.257041569
GTGTAGG65650.06.23768270
TCGGTGG6200.00641445356.20973568
ACTCGCG7409.394426E-46.148705527
Produced by FastQC (version 0.11.5)