FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WRKY40_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WRKY40_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216646
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG10450.013.39725470
AGCGCGG2459.7183324E-411.428678570
CGGAAGC22300.010.82969870
AGAGCGG16950.010.11808970
CTCGGGG3000.00522676959.33342170
GAGCGTG5502.5342852E-68.90917470
GTAGGGG7553.841342E-88.34444870
AGTGTAG22050.07.777850670
TCGTGTT30900.07.36252970
CCCTGCC5400.00150644417.12958565
GGGGAGG4950.00511120547.07077370
CCTGCCC5600.0022120586.874957666
GTAGGGT11351.6076228E-86.78420470
GTCGTGG47250.06.66672970
GTAGGGA41450.06.5018767
GTGTAGA20450.06.332577769
GAGTGTA26650.06.30399969
GGAGGGA18950.06.279741369
TCGGTGG6150.00589691946.26022168
GTGTAGG65800.06.223462670